XYLITOL PRODUCTION BY RECOMBINANT SACCHAROMYCES-CEREVISIAE

J HALLBORN; M WALFRIDSSON; U AIRAKSINEN; H OJAMO; B HAHNHAGERDAL; M PENTTILA; S KERANEN

XYLITOL PRODUCTION BY RECOMBINANT SACCHAROMYCES-CEREVISIAE

J HALLBORN^*
, M WALFRIDSSON
, U AIRAKSINEN
, H OJAMO
, B HAHNHAGERDAL
, M PENTTILA
, S KERANEN

^*Tämän työn vastaava kirjoittaja

Ei julkaistu Aalto-yliopiston affiliaatiolla

Lund University

Tutkimustuotos: Lehtiartikkeli › Article › Scientific › vertaisarvioitu

155 Viittaukset (Web of Science)

Abstrakti

We obtained efficient conversion of xylose to xylitol by transforming Saccharomyces cerevisiae with the gene encoding the xylose reductase (XR) of Pichia stipitis CBS 6054. Comparison of the chromosomal and cDNA copies of the XYL1 gene showed that the genomic XYL1 contains no introns, and an XR monomer of 318 amino acids (35,985 D) is encoded by an open reading frame of 954 bp. The amino acid sequence of the P. stipitis XR is similar to several aldose reductases, suggesting that P. stipitis XR is part of the aldoketo reductase superfamily. S. cerevisiae transformed with the XYL1 gene gave over 95% conversion of xylose into xylitol, a yield not obtainable with natural xylose utilizing yeasts.

Alkuperäiskieli	Englanti
Sivut	1090-1095
Sivumäärä	6
Julkaisu	Bio-Technology
Vuosikerta	9
Numero	11
Tila	Julkaistu - marrask. 1991
OKM-julkaisutyyppi	A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Siteeraa tätä

@article{dfad0984930c47c790fbbd01b3905329,

title = "XYLITOL PRODUCTION BY RECOMBINANT SACCHAROMYCES-CEREVISIAE",

abstract = "We obtained efficient conversion of xylose to xylitol by transforming Saccharomyces cerevisiae with the gene encoding the xylose reductase (XR) of Pichia stipitis CBS 6054. Comparison of the chromosomal and cDNA copies of the XYL1 gene showed that the genomic XYL1 contains no introns, and an XR monomer of 318 amino acids (35,985 D) is encoded by an open reading frame of 954 bp. The amino acid sequence of the P. stipitis XR is similar to several aldose reductases, suggesting that P. stipitis XR is part of the aldoketo reductase superfamily. S. cerevisiae transformed with the XYL1 gene gave over 95\% conversion of xylose into xylitol, a yield not obtainable with natural xylose utilizing yeasts.",

keywords = "XYLOSE REDUCTASE, PACHYSOLEN-TANNOPHILUS, ALDOSE REDUCTASE, CANDIDA-SHEHATAE, PICHIA-STIPITIS, YEASTS, FERMENTATION, PURIFICATION, SUPERFAMILY, CRYSTALLIN",

author = "J HALLBORN and M WALFRIDSSON and U AIRAKSINEN and H OJAMO and B HAHNHAGERDAL and M PENTTILA and S KERANEN",

year = "1991",

month = nov,

language = "English",

volume = "9",

pages = "1090--1095",

journal = "Bio-Technology",

issn = "0733-222X",

publisher = "Nature Publishing Group",

number = "11",

}

TY - JOUR

T1 - XYLITOL PRODUCTION BY RECOMBINANT SACCHAROMYCES-CEREVISIAE

AU - HALLBORN, J

AU - WALFRIDSSON, M

AU - AIRAKSINEN, U

AU - OJAMO, H

AU - HAHNHAGERDAL, B

AU - PENTTILA, M

AU - KERANEN, S

PY - 1991/11

Y1 - 1991/11

N2 - We obtained efficient conversion of xylose to xylitol by transforming Saccharomyces cerevisiae with the gene encoding the xylose reductase (XR) of Pichia stipitis CBS 6054. Comparison of the chromosomal and cDNA copies of the XYL1 gene showed that the genomic XYL1 contains no introns, and an XR monomer of 318 amino acids (35,985 D) is encoded by an open reading frame of 954 bp. The amino acid sequence of the P. stipitis XR is similar to several aldose reductases, suggesting that P. stipitis XR is part of the aldoketo reductase superfamily. S. cerevisiae transformed with the XYL1 gene gave over 95% conversion of xylose into xylitol, a yield not obtainable with natural xylose utilizing yeasts.

AB - We obtained efficient conversion of xylose to xylitol by transforming Saccharomyces cerevisiae with the gene encoding the xylose reductase (XR) of Pichia stipitis CBS 6054. Comparison of the chromosomal and cDNA copies of the XYL1 gene showed that the genomic XYL1 contains no introns, and an XR monomer of 318 amino acids (35,985 D) is encoded by an open reading frame of 954 bp. The amino acid sequence of the P. stipitis XR is similar to several aldose reductases, suggesting that P. stipitis XR is part of the aldoketo reductase superfamily. S. cerevisiae transformed with the XYL1 gene gave over 95% conversion of xylose into xylitol, a yield not obtainable with natural xylose utilizing yeasts.

KW - XYLOSE REDUCTASE

KW - PACHYSOLEN-TANNOPHILUS

KW - ALDOSE REDUCTASE

KW - CANDIDA-SHEHATAE

KW - PICHIA-STIPITIS

KW - YEASTS

KW - FERMENTATION

KW - PURIFICATION

KW - SUPERFAMILY

KW - CRYSTALLIN

M3 - Article

SN - 0733-222X

VL - 9

SP - 1090

EP - 1095

JO - Bio-Technology

JF - Bio-Technology

IS - 11

ER -

XYLITOL PRODUCTION BY RECOMBINANT SACCHAROMYCES-CEREVISIAE

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