Trapping and immobilization of DNA molecules between nanoelectrodes

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Trapping and immobilization of DNA molecules between nanoelectrodes. / Kuzyk, Anton; Toppari, J. Jussi; Törmä, Päivi.

julkaisussa: Methods in molecular biology (Clifton, N.J.), Vuosikerta 749, 01.12.2011, s. 223-234.

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

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Bibtex - Lataa

@article{da318401c7114278a6fd7ce2ce3ae7cd,
title = "Trapping and immobilization of DNA molecules between nanoelectrodes",
abstract = "DNA is one of the most promising molecules for nanoscale bottom-up fabrication. For both scientific studies and fabrication of devices, it is desirable to be able to manipulate DNA molecules, or self-assembled DNA constructions, at the single unit level. Efficient methods are needed for precisely attaching the single unit to the external measurement setup or the device structure. So far, this has often been too cumbersome to achieve, and consequently most of the scientific studies are based on a statistical analysis or measurements done for a sample containing numerous molecules in liquid or in a dry state. Here, we explain a method for trapping and attaching nanoscale double-stranded DNA (dsDNA) molecules between nanoelectrodes. The method is based on dielectrophoresis and gives a high yield of trapping only single or a few molecules, which enables, for example, transport measurements at the single -molecule level. The method has been used to trap different dsDNA fragments, sizes varying from 27 to 8,416 bp, and also DNA origami constructions. We also explain how confocal microscopy can be used to determine and optimize the trapping parameters.",
author = "Anton Kuzyk and Toppari, {J. Jussi} and P{\"a}ivi T{\"o}rm{\"a}",
year = "2011",
month = "12",
day = "1",
language = "English",
volume = "749",
pages = "223--234",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS - Lataa

TY - JOUR

T1 - Trapping and immobilization of DNA molecules between nanoelectrodes

AU - Kuzyk, Anton

AU - Toppari, J. Jussi

AU - Törmä, Päivi

PY - 2011/12/1

Y1 - 2011/12/1

N2 - DNA is one of the most promising molecules for nanoscale bottom-up fabrication. For both scientific studies and fabrication of devices, it is desirable to be able to manipulate DNA molecules, or self-assembled DNA constructions, at the single unit level. Efficient methods are needed for precisely attaching the single unit to the external measurement setup or the device structure. So far, this has often been too cumbersome to achieve, and consequently most of the scientific studies are based on a statistical analysis or measurements done for a sample containing numerous molecules in liquid or in a dry state. Here, we explain a method for trapping and attaching nanoscale double-stranded DNA (dsDNA) molecules between nanoelectrodes. The method is based on dielectrophoresis and gives a high yield of trapping only single or a few molecules, which enables, for example, transport measurements at the single -molecule level. The method has been used to trap different dsDNA fragments, sizes varying from 27 to 8,416 bp, and also DNA origami constructions. We also explain how confocal microscopy can be used to determine and optimize the trapping parameters.

AB - DNA is one of the most promising molecules for nanoscale bottom-up fabrication. For both scientific studies and fabrication of devices, it is desirable to be able to manipulate DNA molecules, or self-assembled DNA constructions, at the single unit level. Efficient methods are needed for precisely attaching the single unit to the external measurement setup or the device structure. So far, this has often been too cumbersome to achieve, and consequently most of the scientific studies are based on a statistical analysis or measurements done for a sample containing numerous molecules in liquid or in a dry state. Here, we explain a method for trapping and attaching nanoscale double-stranded DNA (dsDNA) molecules between nanoelectrodes. The method is based on dielectrophoresis and gives a high yield of trapping only single or a few molecules, which enables, for example, transport measurements at the single -molecule level. The method has been used to trap different dsDNA fragments, sizes varying from 27 to 8,416 bp, and also DNA origami constructions. We also explain how confocal microscopy can be used to determine and optimize the trapping parameters.

UR - http://www.scopus.com/inward/record.url?scp=84855182797&partnerID=8YFLogxK

UR - https://www.ncbi.nlm.nih.gov/pubmed/21674376

M3 - Article

VL - 749

SP - 223

EP - 234

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

ID: 30234453