Three-Dimensional Protein Cage Array Capable of Active Enzyme Capture and Artificial Chaperone Activity

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Three-Dimensional Protein Cage Array Capable of Active Enzyme Capture and Artificial Chaperone Activity. / Chakraborti, Soumyananda; Korpi, Antti; Kumar, Mantu; Stȩpień, Piotr; Kostiainen, Mauri A.; Heddle, Jonathan G.

julkaisussa: Nano Letters, Vuosikerta 19, Nro 6, 06.2019, s. 3918-3924.

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

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Chakraborti, Soumyananda ; Korpi, Antti ; Kumar, Mantu ; Stȩpień, Piotr ; Kostiainen, Mauri A. ; Heddle, Jonathan G. / Three-Dimensional Protein Cage Array Capable of Active Enzyme Capture and Artificial Chaperone Activity. Julkaisussa: Nano Letters. 2019 ; Vuosikerta 19, Nro 6. Sivut 3918-3924.

Bibtex - Lataa

@article{08043b0da32141f5ba42f64d82e12946,
title = "Three-Dimensional Protein Cage Array Capable of Active Enzyme Capture and Artificial Chaperone Activity",
abstract = "Development of protein cages for encapsulation of active enzyme cargoes and their subsequent arrangement into a controllable three-dimensional array is highly desirable. However, cargo capture is typically challenging because of difficulties in achieving reversible assembly/disassembly of protein cages in mild conditions. Herein we show that by using an unusual ferritin cage protein that undergoes triggerable assembly under mild conditions, we can achieve reversible filling with protein cargoes including an active enzyme. We demonstrate that these filled cages can be arrayed in three-dimensional crystal lattices and have an additional chaperone-like effect, increasing both thermostability and enzymatic activity of the encapsulated enzyme.",
keywords = "3D enzyme array, chaperone activity, enzyme encapsulation, Ferritin, ferritin superlattice, NANOREACTOR, COCRYSTALS, ARCHAEOGLOBUS-FULGIDUS FERRITIN, ENCAPSULATION, APOFERRITIN, RESIDUES",
author = "Soumyananda Chakraborti and Antti Korpi and Mantu Kumar and Piotr Stȩpień and Kostiainen, {Mauri A.} and Heddle, {Jonathan G.}",
year = "2019",
month = "6",
doi = "10.1021/acs.nanolett.9b01148",
language = "English",
volume = "19",
pages = "3918--3924",
journal = "Nano Letters",
issn = "1530-6984",
publisher = "AMERICAN CHEMICAL SOCIETY",
number = "6",

}

RIS - Lataa

TY - JOUR

T1 - Three-Dimensional Protein Cage Array Capable of Active Enzyme Capture and Artificial Chaperone Activity

AU - Chakraborti, Soumyananda

AU - Korpi, Antti

AU - Kumar, Mantu

AU - Stȩpień, Piotr

AU - Kostiainen, Mauri A.

AU - Heddle, Jonathan G.

PY - 2019/6

Y1 - 2019/6

N2 - Development of protein cages for encapsulation of active enzyme cargoes and their subsequent arrangement into a controllable three-dimensional array is highly desirable. However, cargo capture is typically challenging because of difficulties in achieving reversible assembly/disassembly of protein cages in mild conditions. Herein we show that by using an unusual ferritin cage protein that undergoes triggerable assembly under mild conditions, we can achieve reversible filling with protein cargoes including an active enzyme. We demonstrate that these filled cages can be arrayed in three-dimensional crystal lattices and have an additional chaperone-like effect, increasing both thermostability and enzymatic activity of the encapsulated enzyme.

AB - Development of protein cages for encapsulation of active enzyme cargoes and their subsequent arrangement into a controllable three-dimensional array is highly desirable. However, cargo capture is typically challenging because of difficulties in achieving reversible assembly/disassembly of protein cages in mild conditions. Herein we show that by using an unusual ferritin cage protein that undergoes triggerable assembly under mild conditions, we can achieve reversible filling with protein cargoes including an active enzyme. We demonstrate that these filled cages can be arrayed in three-dimensional crystal lattices and have an additional chaperone-like effect, increasing both thermostability and enzymatic activity of the encapsulated enzyme.

KW - 3D enzyme array

KW - chaperone activity

KW - enzyme encapsulation

KW - Ferritin

KW - ferritin superlattice

KW - NANOREACTOR

KW - COCRYSTALS

KW - ARCHAEOGLOBUS-FULGIDUS FERRITIN

KW - ENCAPSULATION

KW - APOFERRITIN

KW - RESIDUES

UR - http://www.scopus.com/inward/record.url?scp=85067051846&partnerID=8YFLogxK

U2 - 10.1021/acs.nanolett.9b01148

DO - 10.1021/acs.nanolett.9b01148

M3 - Article

AN - SCOPUS:85067051846

VL - 19

SP - 3918

EP - 3924

JO - Nano Letters

JF - Nano Letters

SN - 1530-6984

IS - 6

ER -

ID: 34835760