Three-Dimensional Protein Cage Array Capable of Active Enzyme Capture and Artificial Chaperone Activity

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Tutkijat

Organisaatiot

  • Jagiellonian University in Kraków

Kuvaus

Development of protein cages for encapsulation of active enzyme cargoes and their subsequent arrangement into a controllable three-dimensional array is highly desirable. However, cargo capture is typically challenging because of difficulties in achieving reversible assembly/disassembly of protein cages in mild conditions. Herein we show that by using an unusual ferritin cage protein that undergoes triggerable assembly under mild conditions, we can achieve reversible filling with protein cargoes including an active enzyme. We demonstrate that these filled cages can be arrayed in three-dimensional crystal lattices and have an additional chaperone-like effect, increasing both thermostability and enzymatic activity of the encapsulated enzyme.

Yksityiskohdat

AlkuperäiskieliEnglanti
Sivut3918-3924
Sivumäärä7
JulkaisuNano Letters
Vuosikerta19
Numero6
TilaJulkaistu - kesäkuuta 2019
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

ID: 34835760