The 'true' L-xylulose reductase of filamentous fungi identified in Aspergillus niger

Dominik Mojzita; Kiira Vuoristo; Outi M. Koivistoinen; Merja Penttila; Peter Richard

doi:10.1016/j.febslet.2010.06.037

The 'true' L-xylulose reductase of filamentous fungi identified in Aspergillus niger

Dominik Mojzita
, Kiira Vuoristo
, Outi M. Koivistoinen
, Merja Penttila
, Peter Richard^*

^*Tämän työn vastaava kirjoittaja

Ei julkaistu Aalto-yliopiston affiliaatiolla

VTT Technical Research Centre of Finland Ltd.

Tutkimustuotos: Lehtiartikkeli › Article › Scientific › vertaisarvioitu

31 Viittaukset (Web of Science)

Abstrakti

L-Xylulose reductase is part of the eukaryotic pathway for L-arabinose catabolism. A previously identified L-xylulose reductase in Hypocrea jecorina turned out to be not the 'true' one since it was not upregulated during growth on L-arabinose and the deletion strain showed no reduced L-xylulose reductase activity but instead lost the D-mannitol dehydrogenase activity [17]. In this communication we identified the 'true' L-xylulose reductase in Aspergillus niger. The gene, lxrA (JGI177736), is upregulated on L-arabinose and the deletion results in a strain lacking the NADPH-specific L-xylulose reductase activity and having reduced growth on L-arabinose. The purified enzyme had a K(m) for L-xylulose of 25 mM and a nu(max) of 650 U/mg. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

Alkuperäiskieli	Englanti
Sivut	3540-3544
Sivumäärä	5
Julkaisu	FEBS Letters
Vuosikerta	584
Numero	16
DOI - pysyväislinkit	https://doi.org/10.1016/j.febslet.2010.06.037
Tila	Julkaistu - 20 elok. 2010
OKM-julkaisutyyppi	A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Rahoitus

This work was financially supported by the Academy of Finland through the research programme "White Biotechnology - Green Chemistry", The Finnish Centre of Excellence Programme, 2008-2013, Project No. 118573 and an Academy Research Fellowship for P. R. We thank Anne Usvalampi for the L-xylulose producing E. coli strain.

Pääsy asiakirjaan

10.1016/j.febslet.2010.06.037

Siteeraa tätä

@article{7018e348f1ff42bfa8904154cfad3a99,

title = "The 'true' L-xylulose reductase of filamentous fungi identified in Aspergillus niger",

abstract = "L-Xylulose reductase is part of the eukaryotic pathway for L-arabinose catabolism. A previously identified L-xylulose reductase in Hypocrea jecorina turned out to be not the 'true' one since it was not upregulated during growth on L-arabinose and the deletion strain showed no reduced L-xylulose reductase activity but instead lost the D-mannitol dehydrogenase activity [17]. In this communication we identified the 'true' L-xylulose reductase in Aspergillus niger. The gene, lxrA (JGI177736), is upregulated on L-arabinose and the deletion results in a strain lacking the NADPH-specific L-xylulose reductase activity and having reduced growth on L-arabinose. The purified enzyme had a K(m) for L-xylulose of 25 mM and a nu(max) of 650 U/mg. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.",

keywords = "EC 1.1.1.10, L-Arabinose metabolism, L-Xylulose, Pentose, Aspergillus niger, L-ARABINOSE METABOLISM, HYPOCREA-JECORINA, CATABOLIC PATHWAY, GENE, DEHYDROGENASE, EXPRESSION, CLONING, PENTOSE, YEAST",

author = "Dominik Mojzita and Kiira Vuoristo and Koivistoinen, \{Outi M.\} and Merja Penttila and Peter Richard",

year = "2010",

month = aug,

day = "20",

doi = "10.1016/j.febslet.2010.06.037",

language = "English",

volume = "584",

pages = "3540--3544",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley",

number = "16",

}

TY - JOUR

T1 - The 'true' L-xylulose reductase of filamentous fungi identified in Aspergillus niger

AU - Mojzita, Dominik

AU - Vuoristo, Kiira

AU - Koivistoinen, Outi M.

AU - Penttila, Merja

AU - Richard, Peter

PY - 2010/8/20

Y1 - 2010/8/20

N2 - L-Xylulose reductase is part of the eukaryotic pathway for L-arabinose catabolism. A previously identified L-xylulose reductase in Hypocrea jecorina turned out to be not the 'true' one since it was not upregulated during growth on L-arabinose and the deletion strain showed no reduced L-xylulose reductase activity but instead lost the D-mannitol dehydrogenase activity [17]. In this communication we identified the 'true' L-xylulose reductase in Aspergillus niger. The gene, lxrA (JGI177736), is upregulated on L-arabinose and the deletion results in a strain lacking the NADPH-specific L-xylulose reductase activity and having reduced growth on L-arabinose. The purified enzyme had a K(m) for L-xylulose of 25 mM and a nu(max) of 650 U/mg. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

AB - L-Xylulose reductase is part of the eukaryotic pathway for L-arabinose catabolism. A previously identified L-xylulose reductase in Hypocrea jecorina turned out to be not the 'true' one since it was not upregulated during growth on L-arabinose and the deletion strain showed no reduced L-xylulose reductase activity but instead lost the D-mannitol dehydrogenase activity [17]. In this communication we identified the 'true' L-xylulose reductase in Aspergillus niger. The gene, lxrA (JGI177736), is upregulated on L-arabinose and the deletion results in a strain lacking the NADPH-specific L-xylulose reductase activity and having reduced growth on L-arabinose. The purified enzyme had a K(m) for L-xylulose of 25 mM and a nu(max) of 650 U/mg. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

KW - EC 1.1.1.10

KW - L-Arabinose metabolism

KW - L-Xylulose

KW - Pentose

KW - Aspergillus niger

KW - L-ARABINOSE METABOLISM

KW - HYPOCREA-JECORINA

KW - CATABOLIC PATHWAY

KW - GENE

KW - DEHYDROGENASE

KW - EXPRESSION

KW - CLONING

KW - PENTOSE

KW - YEAST

U2 - 10.1016/j.febslet.2010.06.037

DO - 10.1016/j.febslet.2010.06.037

M3 - Article

SN - 0014-5793

VL - 584

SP - 3540

EP - 3544

JO - FEBS Letters

JF - FEBS Letters

IS - 16

ER -

The 'true' L-xylulose reductase of filamentous fungi identified in Aspergillus niger

Abstrakti

Rahoitus

Pääsy asiakirjaan

Sormenjälki

Siteeraa tätä