TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells

Tutkimustuotos: Lehtiartikkeli

Tutkijat

  • Ageliki Tsagaratou
  • Edahí González-Avalos
  • Sini Rautio

  • James P. Scott-Browne
  • Susan Togher
  • William A. Pastor
  • Ellen V. Rothenberg
  • Lukas Chavez
  • Harri Lähdesmäki

  • Anjana Rao

Organisaatiot

  • La Jolla Institute for Allergy and Immunology
  • California Institute of Technology
  • German Cancer Research Center
  • University of California at San Diego

Kuvaus

TET proteins oxidize 5-methylcytosine in DNA to 5-hydroxymethylcytosine and other oxidation products. We found that simultaneous deletion of Tet2 and Tet3 in mouse CD4+CD8+ double-positive thymocytes resulted in dysregulated development and proliferation of invariant natural killer T cells (iNKT cells). Tet2-Tet3 double-knockout (DKO) iNKT cells displayed pronounced skewing toward the NKT17 lineage, with increased DNA methylation and impaired expression of genes encoding the key lineage-specifying factors T-bet and ThPOK. Transfer of purified Tet2-Tet3 DKO iNKT cells into immunocompetent recipient mice resulted in an uncontrolled expansion that was dependent on the nonclassical major histocompatibility complex (MHC) protein CD1d, which presents lipid antigens to iNKT cells. Our data indicate that TET proteins regulate iNKT cell fate by ensuring their proper development and maturation and by suppressing aberrant proliferation mediated by the T cell antigen receptor (TCR).

Yksityiskohdat

AlkuperäiskieliEnglanti
Sivut45-53
JulkaisuNATURE IMMUNOLOGY
Vuosikerta18
Numero1
TilaJulkaistu - 2017
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

ID: 9730061