Projekteja vuodessa
Abstrakti
Despite impressive progress in the field, there are still several major bottlenecks in producing fibers from recombinantly produced spider-silk-like proteins to replicate the extraordinary mechanical properties of spider major ampullate silk. The conventional artificial fiber spinning processes rely primarily on organic solvents to coagulate proteins into fibers and require complex post-treatments to obtain fibers with valuable properties. This is due to challenges in obtaining soluble silk proteins, but also because the native silk spinning process leading to the hierarchical organization of the silk proteins is not fully understood and is hard to replicate in a manner applicable to industrial settings. Here, recombinant spider-silk fusion proteins are efficiently produced and processed into as-spun fibers with a toughness modulus of 120 MJ m−3 and extensibility of 255% using solely aqueous solutions. The spider-silk fusion proteins assemble in a manner similar to that reported for native spider silk: they phase separate induced by salting out, followed by alignment and a secondary structure transition triggered by shear forces and dehydration. Finally, the design of the fusion silk proteins enables straightforward functionalization of the fibers under mild all-aqueous conditions via a simple biomolecular click reaction both pre- and post-spinning.
Alkuperäiskieli | Englanti |
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Artikkeli | 2410415 |
Julkaisu | Advanced Functional Materials |
DOI - pysyväislinkit | |
Tila | Sähköinen julkaisu (e-pub) ennen painettua julkistusta - 26 heinäk. 2024 |
OKM-julkaisutyyppi | A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä |
Sormenjälki
Sukella tutkimusaiheisiin 'Sustainable Spinning of Artificial Spider Silk Fibers with Excellent Toughness and Inherent Potential for Functionalization'. Ne muodostavat yhdessä ainutlaatuisen sormenjäljen.-
Pro2Fun: From post-translationally modified proteins to functional biomaterials
Aranko, S. (Vastuullinen tutkija), Knuuttila, K. (Projektin jäsen), Möttönen, N. (Projektin jäsen), Fan, R. (Projektin jäsen), Tikka, M. (Projektin jäsen), Jokio, J. (Projektin jäsen), Shen, M. (Projektin jäsen) & Rahimipour, A. (Projektin jäsen)
01/10/2023 → 30/09/2028
Projekti: Other external funding: Other foreign funding
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LIBER Linder: Life-like hybrid materials
Linder, M. (Vastuullinen tutkija), Elfving, K. (Projektin jäsen), Lemetti, L. (Projektin jäsen), Tunn, I. (Projektin jäsen), Aspelin, H. (Projektin jäsen), Roas Escalona, N. (Projektin jäsen), Aranko, S. (Projektin jäsen), Malkamäki, M. (Projektin jäsen), Osmekhina, E. (Projektin jäsen) & Fedorov, D. (Projektin jäsen)
01/01/2022 → 31/12/2024
Projekti: Academy of Finland: Other research funding
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CrossSilk: Durable and functional biomaterials from crosslinked non-canonical silk and cellulose composite
Aranko, S. (Vastuullinen tutkija), Elfving, K. (Projektin jäsen), Shen, M. (Projektin jäsen), Fan, R. (Projektin jäsen) & Astapov, D. (Projektin jäsen)
01/09/2020 → 31/08/2024
Projekti: Academy of Finland: Other research funding