Surface initiated polymerization of a cationic monomer on inner surfaces of silica capillaries: Analyte separation by capillary electrophoresis versus polyelectrolyte behavior

Joanna Witos, Mikko Karesoja, Erno Karjalainen, Heikki Tenhu, Marja Liisa Riekkola*

*Tämän työn vastaava kirjoittaja

Tutkimustuotos: LehtiartikkeliArticleScientificvertaisarvioitu

14 Sitaatiot (Scopus)

Abstrakti

[2-(Methacryloyl)oxyethyl]trimethylammonium chloride was successfully polymerized by surface-initiated atom transfer radical polymerization method on the inner surface of fused-silica capillaries resulting in a covalently bound poly([2-(methacryloyl)oxyethyl]trimethylammonium chloride) coating. The coated capillaries provided in capillary electrophoresis an excellent run-to-run repeatability, capillary-to-capillary and day-to-day reproducibility. The capillaries worked reliably over 1 month with EOF repeatability below 0.5%. The positively charged coated capillaries were successfully applied to the capillary electrophoretic separation of three standard proteins and five β-blockers with the separation efficiencies ranging from 132 000 to 303 000 plates/m, and from 82 000 to 189 000 plates/m, respectively. In addition, challenging high- and low-density lipoprotein particles could be separated. The hydrodynamic sizes of free polymer chains in buffers used in the capillary electrophoretic experiments were measured for the characterization of the coatings.

AlkuperäiskieliEnglanti
Sivut1070-1077
Sivumäärä8
JulkaisuJournal of Separation Science
Vuosikerta36
Numero6
DOI - pysyväislinkit
TilaJulkaistu - 1 maalisk. 2013
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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