Structural diversity and bioactivities of peptaibol compounds from the longibrachiatum clade of the filamentous fungal genus trichoderma

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Standard

Structural diversity and bioactivities of peptaibol compounds from the longibrachiatum clade of the filamentous fungal genus trichoderma. / Marik, Tamás; Tyagi, Chetna; Balázs, Dóra; Urbán, Péter; Szepesi, Ágnes; Bakacsy, László; Endre, Gábor; Rakk, Dávid; Szekeres, András; Andersson, Maria A.; Salonen, Heidi; Druzhinina, Irina S.; Vágvölgyi, Csaba; Kredics, László.

julkaisussa: Frontiers in Microbiology, Vuosikerta 10, Nro JUN, 1434, 01.01.2019.

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Harvard

Marik, T, Tyagi, C, Balázs, D, Urbán, P, Szepesi, Á, Bakacsy, L, Endre, G, Rakk, D, Szekeres, A, Andersson, MA, Salonen, H, Druzhinina, IS, Vágvölgyi, C & Kredics, L 2019, 'Structural diversity and bioactivities of peptaibol compounds from the longibrachiatum clade of the filamentous fungal genus trichoderma' Frontiers in Microbiology, Vuosikerta. 10, Nro JUN, 1434. https://doi.org/10.3389/fmicb.2019.01434

APA

Vancouver

Author

Marik, Tamás ; Tyagi, Chetna ; Balázs, Dóra ; Urbán, Péter ; Szepesi, Ágnes ; Bakacsy, László ; Endre, Gábor ; Rakk, Dávid ; Szekeres, András ; Andersson, Maria A. ; Salonen, Heidi ; Druzhinina, Irina S. ; Vágvölgyi, Csaba ; Kredics, László. / Structural diversity and bioactivities of peptaibol compounds from the longibrachiatum clade of the filamentous fungal genus trichoderma. Julkaisussa: Frontiers in Microbiology. 2019 ; Vuosikerta 10, Nro JUN.

Bibtex - Lataa

@article{6d1e608d71c9429d8d0642c6ed3974e6,
title = "Structural diversity and bioactivities of peptaibol compounds from the longibrachiatum clade of the filamentous fungal genus trichoderma",
abstract = "This study examined the structural diversity and bioactivity of peptaibol compounds produced by species from the phylogenetically separated Longibrachiatum Clade of the filamentous fungal genus Trichoderma, which contains several biotechnologically, agriculturally and clinically important species. HPLC-ESI-MS investigations of crude extracts from 17 species of the Longibrachiatum Clade (T. aethiopicum, T. andinense, T. capillare, T. citrinoviride, T. effusum, T. flagellatum, T. ghanense, T. konilangbra, T. longibrachiatum, T. novae-zelandiae, T. pinnatum, T. parareesei, T. pseudokoningii, T. reesei, T. saturnisporum, T. sinensis, and T. orientale) revealed several new and recurrent 20-residue peptaibols related to trichobrachins, paracelsins, suzukacillins, saturnisporins, trichoaureocins, trichocellins, longibrachins, hyporientalins, trichokonins, trilongins, metanicins, trichosporins, gliodeliquescins, alamethicins and hypophellins, as well as eight 19-residue sequences from a new subfamily of peptaibols named brevicelsins. Non-ribosomal peptide synthetase genes were mined from the available genome sequences of the Longibrachiatum Clade. Their annotation and product prediction were performed in silico and revealed full agreement in 11 out of 20 positions regarding the amino acids predicted based on the signature sequences and the detected amino acids incorporated. Molecular dynamics simulations were performed for structural characterization of four selected peptaibol sequences: paracelsins B, H and their 19-residue counterparts brevicelsins I and IV. Loss of position R6 in brevicelsins resulted in smaller helical structures with higher atomic fluctuation for every residue than the structures formed by paracelsins. We observed the formation of highly bent, almost hairpin-like, helical structures throughout the trajectory, along with linear conformation. Bioactivity tests were performed on the purified peptaibol extract of T. reesei on clinically and phytopathologically important filamentous fungi, mammalian cells, and Arabidopsis thaliana seedlings. Porcine kidney cells and boar spermatozoa proved to be sensitive to the purified peptaibol extract. Peptaibol concentrations =0.3 mg ml-1 deterred the growth of A. thaliana. However, negative effects to plants were not detected at concentrations below 0.1 mg ml-1, which could still inhibit plant pathogenic filamentous fungi, suggesting that those peptaibols reported here may have applications for plant protection.",
keywords = "Antifungal activity, Arabidopsis, Brevicelsin, Longibrachiatum, Mammalian cells, Mass spectrometry, Peptaibol, Trichoderma",
author = "Tam{\'a}s Marik and Chetna Tyagi and D{\'o}ra Bal{\'a}zs and P{\'e}ter Urb{\'a}n and {\'A}gnes Szepesi and L{\'a}szl{\'o} Bakacsy and G{\'a}bor Endre and D{\'a}vid Rakk and Andr{\'a}s Szekeres and Andersson, {Maria A.} and Heidi Salonen and Druzhinina, {Irina S.} and Csaba V{\'a}gv{\"o}lgyi and L{\'a}szl{\'o} Kredics",
year = "2019",
month = "1",
day = "1",
doi = "10.3389/fmicb.2019.01434",
language = "English",
volume = "10",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Research Foundation",
number = "JUN",

}

RIS - Lataa

TY - JOUR

T1 - Structural diversity and bioactivities of peptaibol compounds from the longibrachiatum clade of the filamentous fungal genus trichoderma

AU - Marik, Tamás

AU - Tyagi, Chetna

AU - Balázs, Dóra

AU - Urbán, Péter

AU - Szepesi, Ágnes

AU - Bakacsy, László

AU - Endre, Gábor

AU - Rakk, Dávid

AU - Szekeres, András

AU - Andersson, Maria A.

AU - Salonen, Heidi

AU - Druzhinina, Irina S.

AU - Vágvölgyi, Csaba

AU - Kredics, László

PY - 2019/1/1

Y1 - 2019/1/1

N2 - This study examined the structural diversity and bioactivity of peptaibol compounds produced by species from the phylogenetically separated Longibrachiatum Clade of the filamentous fungal genus Trichoderma, which contains several biotechnologically, agriculturally and clinically important species. HPLC-ESI-MS investigations of crude extracts from 17 species of the Longibrachiatum Clade (T. aethiopicum, T. andinense, T. capillare, T. citrinoviride, T. effusum, T. flagellatum, T. ghanense, T. konilangbra, T. longibrachiatum, T. novae-zelandiae, T. pinnatum, T. parareesei, T. pseudokoningii, T. reesei, T. saturnisporum, T. sinensis, and T. orientale) revealed several new and recurrent 20-residue peptaibols related to trichobrachins, paracelsins, suzukacillins, saturnisporins, trichoaureocins, trichocellins, longibrachins, hyporientalins, trichokonins, trilongins, metanicins, trichosporins, gliodeliquescins, alamethicins and hypophellins, as well as eight 19-residue sequences from a new subfamily of peptaibols named brevicelsins. Non-ribosomal peptide synthetase genes were mined from the available genome sequences of the Longibrachiatum Clade. Their annotation and product prediction were performed in silico and revealed full agreement in 11 out of 20 positions regarding the amino acids predicted based on the signature sequences and the detected amino acids incorporated. Molecular dynamics simulations were performed for structural characterization of four selected peptaibol sequences: paracelsins B, H and their 19-residue counterparts brevicelsins I and IV. Loss of position R6 in brevicelsins resulted in smaller helical structures with higher atomic fluctuation for every residue than the structures formed by paracelsins. We observed the formation of highly bent, almost hairpin-like, helical structures throughout the trajectory, along with linear conformation. Bioactivity tests were performed on the purified peptaibol extract of T. reesei on clinically and phytopathologically important filamentous fungi, mammalian cells, and Arabidopsis thaliana seedlings. Porcine kidney cells and boar spermatozoa proved to be sensitive to the purified peptaibol extract. Peptaibol concentrations =0.3 mg ml-1 deterred the growth of A. thaliana. However, negative effects to plants were not detected at concentrations below 0.1 mg ml-1, which could still inhibit plant pathogenic filamentous fungi, suggesting that those peptaibols reported here may have applications for plant protection.

AB - This study examined the structural diversity and bioactivity of peptaibol compounds produced by species from the phylogenetically separated Longibrachiatum Clade of the filamentous fungal genus Trichoderma, which contains several biotechnologically, agriculturally and clinically important species. HPLC-ESI-MS investigations of crude extracts from 17 species of the Longibrachiatum Clade (T. aethiopicum, T. andinense, T. capillare, T. citrinoviride, T. effusum, T. flagellatum, T. ghanense, T. konilangbra, T. longibrachiatum, T. novae-zelandiae, T. pinnatum, T. parareesei, T. pseudokoningii, T. reesei, T. saturnisporum, T. sinensis, and T. orientale) revealed several new and recurrent 20-residue peptaibols related to trichobrachins, paracelsins, suzukacillins, saturnisporins, trichoaureocins, trichocellins, longibrachins, hyporientalins, trichokonins, trilongins, metanicins, trichosporins, gliodeliquescins, alamethicins and hypophellins, as well as eight 19-residue sequences from a new subfamily of peptaibols named brevicelsins. Non-ribosomal peptide synthetase genes were mined from the available genome sequences of the Longibrachiatum Clade. Their annotation and product prediction were performed in silico and revealed full agreement in 11 out of 20 positions regarding the amino acids predicted based on the signature sequences and the detected amino acids incorporated. Molecular dynamics simulations were performed for structural characterization of four selected peptaibol sequences: paracelsins B, H and their 19-residue counterparts brevicelsins I and IV. Loss of position R6 in brevicelsins resulted in smaller helical structures with higher atomic fluctuation for every residue than the structures formed by paracelsins. We observed the formation of highly bent, almost hairpin-like, helical structures throughout the trajectory, along with linear conformation. Bioactivity tests were performed on the purified peptaibol extract of T. reesei on clinically and phytopathologically important filamentous fungi, mammalian cells, and Arabidopsis thaliana seedlings. Porcine kidney cells and boar spermatozoa proved to be sensitive to the purified peptaibol extract. Peptaibol concentrations =0.3 mg ml-1 deterred the growth of A. thaliana. However, negative effects to plants were not detected at concentrations below 0.1 mg ml-1, which could still inhibit plant pathogenic filamentous fungi, suggesting that those peptaibols reported here may have applications for plant protection.

KW - Antifungal activity

KW - Arabidopsis

KW - Brevicelsin

KW - Longibrachiatum

KW - Mammalian cells

KW - Mass spectrometry

KW - Peptaibol

KW - Trichoderma

UR - http://www.scopus.com/inward/record.url?scp=85069043718&partnerID=8YFLogxK

U2 - 10.3389/fmicb.2019.01434

DO - 10.3389/fmicb.2019.01434

M3 - Article

VL - 10

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

IS - JUN

M1 - 1434

ER -

ID: 35766210