TY - JOUR
T1 - Structural characterization of corn fiber hemicelluloses extracted by organic solvent and screening of degradation enzymes
AU - Guo, Yingjie
AU - Zhang, Zheng
AU - Dou, Jinze
AU - Liu, Guodong
AU - Li, Xuezhi
AU - Zhao, Jian
N1 - Funding Information:
We would like to thank Prof. Dmitry Evtuguin from University of Aveiro for help in purification of hemicellulose, and Haiyan Sui from State Key Laboratory of Microbial Technology of Shandong University for help in NMR analysis. This study is financially supported by the National Key Research and Development Program of China (2022YFC3401700) and Natural Science Foundation of Shandong Province, China (ZR2019ZD19).
Publisher Copyright:
© 2023
PY - 2023/8/1
Y1 - 2023/8/1
N2 - An integrated treatment coupling peracetic acid delignification, dimethyl sulfoxide extraction, and ethanol precipitation were performed to isolate hemicellulose from de-starched corn fiber. Based on chemical composition, molecular weight distribution, methylation, and nuclear magnetic resonance spectroscopy, it is proposed that hemicelluloses in corn fiber were composed of two polysaccharides, glucuronoarabinoxylan (about 80 %) and xyloglucan (about 20 %). Xylose (about 46 %) and arabinose (about 32 %) were the main components in glucuronoarabinoxylan. More than half of the xylose units in the glucuronoarabinoxylan backbone chain were substituted at O-2 and/or O-3 by various monomers or oligomeric side chains. Based on structure analysis, five hemicellulases were selected and added to Penicillium oxalicum MCAX enzymes for enzymatic hydrolysis of corn fiber. The results showed that the addition of hemicellulases increased the sugar yield of corn fiber. These results demonstrate the effectiveness of enzyme consortium constructed by elucidating the chemical structure of hemicellulose in corn fiber for the degradation of corn fiber and also provide a general solution for the rational construction of targeted and efficient enzyme systems for the degradation of lignocellulosic biomass.
AB - An integrated treatment coupling peracetic acid delignification, dimethyl sulfoxide extraction, and ethanol precipitation were performed to isolate hemicellulose from de-starched corn fiber. Based on chemical composition, molecular weight distribution, methylation, and nuclear magnetic resonance spectroscopy, it is proposed that hemicelluloses in corn fiber were composed of two polysaccharides, glucuronoarabinoxylan (about 80 %) and xyloglucan (about 20 %). Xylose (about 46 %) and arabinose (about 32 %) were the main components in glucuronoarabinoxylan. More than half of the xylose units in the glucuronoarabinoxylan backbone chain were substituted at O-2 and/or O-3 by various monomers or oligomeric side chains. Based on structure analysis, five hemicellulases were selected and added to Penicillium oxalicum MCAX enzymes for enzymatic hydrolysis of corn fiber. The results showed that the addition of hemicellulases increased the sugar yield of corn fiber. These results demonstrate the effectiveness of enzyme consortium constructed by elucidating the chemical structure of hemicellulose in corn fiber for the degradation of corn fiber and also provide a general solution for the rational construction of targeted and efficient enzyme systems for the degradation of lignocellulosic biomass.
KW - Corn fiber
KW - Glucuronoarabinoxylan
KW - Hemicellulose
KW - Organic solvent extraction
KW - Structural characterization
UR - http://www.scopus.com/inward/record.url?scp=85151544106&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2023.120820
DO - 10.1016/j.carbpol.2023.120820
M3 - Article
AN - SCOPUS:85151544106
SN - 0144-8617
VL - 313
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 120820
ER -
