Single-molecule kinetics and super-resolution microscopy by fluorescence imaging of transient binding on DNA origami

Tutkimustuotos: Lehtiartikkeli

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Single-molecule kinetics and super-resolution microscopy by fluorescence imaging of transient binding on DNA origami. / Jungmann, Ralf; Steinhauer, Christian; Scheible, Max; Kuzyk, Anton; Tinnefeld, Philip; Simmel, Friedrich C.

julkaisussa: Nano Letters, Vuosikerta 10, Nro 11, 10.11.2010, s. 4756-4761.

Tutkimustuotos: Lehtiartikkeli

Harvard

Jungmann, R, Steinhauer, C, Scheible, M, Kuzyk, A, Tinnefeld, P & Simmel, FC 2010, 'Single-molecule kinetics and super-resolution microscopy by fluorescence imaging of transient binding on DNA origami', Nano Letters, Vuosikerta. 10, Nro 11, Sivut 4756-4761. https://doi.org/10.1021/nl103427w

APA

Vancouver

Author

Jungmann, Ralf ; Steinhauer, Christian ; Scheible, Max ; Kuzyk, Anton ; Tinnefeld, Philip ; Simmel, Friedrich C. / Single-molecule kinetics and super-resolution microscopy by fluorescence imaging of transient binding on DNA origami. Julkaisussa: Nano Letters. 2010 ; Vuosikerta 10, Nro 11. Sivut 4756-4761.

Bibtex - Lataa

@article{c87397daa327413da525063efd1d9efc,
title = "Single-molecule kinetics and super-resolution microscopy by fluorescence imaging of transient binding on DNA origami",
abstract = "DNA origami is a powerful method for the programmable assembly of nanoscale molecular structures. For applications of these structures as functional biomaterials, the study of reaction kinetics and dynamic processes in real time and with high spatial resolution becomes increasingly important. We present a single-molecule assay for the study of binding and unbinding kinetics on DNA origami. We find that the kinetics of hybridization to single-stranded extensions on DNA origami is similar to isolated substrate-immobilized DNA with a slight position dependence on the origami. On the basis of the knowledge of the kinetics, we exploit reversible specific binding of labeled oligonucleotides to DNA nanostructures for PAINT (points accumulation for imaging in nanoscale topography) imaging with <30 nm resolution. The method is demonstrated for flat monomeric DNA structures as well as multimeric, ribbon-like DNA structures.",
keywords = "biophysics, DNA origami, fluorescence microscopy, Nanobiotechnology, single-molecule kinetics, super-resolution",
author = "Ralf Jungmann and Christian Steinhauer and Max Scheible and Anton Kuzyk and Philip Tinnefeld and Simmel, {Friedrich C.}",
year = "2010",
month = "11",
day = "10",
doi = "10.1021/nl103427w",
language = "English",
volume = "10",
pages = "4756--4761",
journal = "Nano Letters",
issn = "1530-6984",
publisher = "AMERICAN CHEMICAL SOCIETY",
number = "11",

}

RIS - Lataa

TY - JOUR

T1 - Single-molecule kinetics and super-resolution microscopy by fluorescence imaging of transient binding on DNA origami

AU - Jungmann, Ralf

AU - Steinhauer, Christian

AU - Scheible, Max

AU - Kuzyk, Anton

AU - Tinnefeld, Philip

AU - Simmel, Friedrich C.

PY - 2010/11/10

Y1 - 2010/11/10

N2 - DNA origami is a powerful method for the programmable assembly of nanoscale molecular structures. For applications of these structures as functional biomaterials, the study of reaction kinetics and dynamic processes in real time and with high spatial resolution becomes increasingly important. We present a single-molecule assay for the study of binding and unbinding kinetics on DNA origami. We find that the kinetics of hybridization to single-stranded extensions on DNA origami is similar to isolated substrate-immobilized DNA with a slight position dependence on the origami. On the basis of the knowledge of the kinetics, we exploit reversible specific binding of labeled oligonucleotides to DNA nanostructures for PAINT (points accumulation for imaging in nanoscale topography) imaging with <30 nm resolution. The method is demonstrated for flat monomeric DNA structures as well as multimeric, ribbon-like DNA structures.

AB - DNA origami is a powerful method for the programmable assembly of nanoscale molecular structures. For applications of these structures as functional biomaterials, the study of reaction kinetics and dynamic processes in real time and with high spatial resolution becomes increasingly important. We present a single-molecule assay for the study of binding and unbinding kinetics on DNA origami. We find that the kinetics of hybridization to single-stranded extensions on DNA origami is similar to isolated substrate-immobilized DNA with a slight position dependence on the origami. On the basis of the knowledge of the kinetics, we exploit reversible specific binding of labeled oligonucleotides to DNA nanostructures for PAINT (points accumulation for imaging in nanoscale topography) imaging with <30 nm resolution. The method is demonstrated for flat monomeric DNA structures as well as multimeric, ribbon-like DNA structures.

KW - biophysics

KW - DNA origami

KW - fluorescence microscopy

KW - Nanobiotechnology

KW - single-molecule kinetics

KW - super-resolution

UR - http://www.scopus.com/inward/record.url?scp=78449290663&partnerID=8YFLogxK

U2 - 10.1021/nl103427w

DO - 10.1021/nl103427w

M3 - Article

VL - 10

SP - 4756

EP - 4761

JO - Nano Letters

JF - Nano Letters

SN - 1530-6984

IS - 11

ER -

ID: 30234673