Single molecule characterization of DNA binding and strand displacement reactions on lithographic DNA origami microarrays

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Tutkijat

  • Max B. Scheible
  • Günther Pardatscher
  • Anton Kuzyk

  • Friedrich C. Simmel

Organisaatiot

  • Nanosystems Initiative Munich
  • Technische Universität München
  • Max Planck Institute for Intelligent Systems

Kuvaus

The combination of molecular self-assembly based on the DNA origami technique with lithographic patterning enables the creation of hierarchically ordered nanosystems, in which single molecules are positioned at precise locations on multiple length scales. Based on a hybrid assembly protocol utilizing DNA self-assembly and electron-beam lithography on transparent glass substrates, we here demonstrate a DNA origami microarray, which is compatible with the requirements of single molecule fluorescence and super-resolution microscopy. The spatial arrangement allows for a simple and reliable identification of single molecule events and facilitates automated read-out and data analysis. As a specific application, we utilize the microarray to characterize the performance of DNA strand displacement reactions localized on the DNA origami structures. We find considerable variability within the array, which results both from structural variations and stochastic reaction dynamics prevalent at the single molecule level.

Yksityiskohdat

AlkuperäiskieliEnglanti
Sivut1627-1633
Sivumäärä7
JulkaisuNano Letters
Vuosikerta14
Numero3
TilaJulkaistu - 12 maaliskuuta 2014
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

ID: 9617699