Silver-Stabilized Guanine Duplex: Structural and Optical Properties

Tutkimustuotos: Lehtiartikkeli

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Bibtex - Lataa

@article{44e63fe2b16d42fe9bb08dee9691d778,
title = "Silver-Stabilized Guanine Duplex: Structural and Optical Properties",
abstract = "Recent experimental duplexes of DNA stabilized by Ag cations, pairing homostrands of guanine-guanine, cytosine-cytosine, adenine-thymine, and thymine-thymine, display much higher stability than the Watson-Crick paired DNA duplexes; these broaden the range of applications for DNA nanotechnology. Here we focus on silver-stabilized guanine duplexes in water. Using hybrid quantum mechanics/molecular mechanics simulations, we propose an atomic structure for the Ag+-mediated guanine duplex with two nucleobases per strand, G2-Ag2+-G2. We then compare experimental and time-dependent density functional theory-simulated electronic circular dichroism (ECD) spectra to validate our results. Both experimental and simulated ECD share two negative peaks around 220 and 280 nm, with no positive signal in the measured wavelength range. We found that the left- or right-handed disposition of bases in the structure has a decisive effect on the signs of the ECD. We conclude that G2-Ag2+-G2 is left-hand-oriented, and extrapolation of this orientation to longer strands gives rise to a left-hand-oriented parallel helix stabilized by interplanar H bonds.",
author = "Xi Chen and Esko Makkonen and Dorothea Golze and Olga Lopez-Acevedo",
year = "2018",
month = "8",
day = "16",
doi = "10.1021/acs.jpclett.8b01908",
language = "English",
volume = "9",
pages = "4789--4794",
journal = "Journal of Physical Chemistry Letters",
issn = "1948-7185",
publisher = "AMERICAN CHEMICAL SOCIETY",
number = "16",

}

RIS - Lataa

TY - JOUR

T1 - Silver-Stabilized Guanine Duplex

T2 - Structural and Optical Properties

AU - Chen, Xi

AU - Makkonen, Esko

AU - Golze, Dorothea

AU - Lopez-Acevedo, Olga

PY - 2018/8/16

Y1 - 2018/8/16

N2 - Recent experimental duplexes of DNA stabilized by Ag cations, pairing homostrands of guanine-guanine, cytosine-cytosine, adenine-thymine, and thymine-thymine, display much higher stability than the Watson-Crick paired DNA duplexes; these broaden the range of applications for DNA nanotechnology. Here we focus on silver-stabilized guanine duplexes in water. Using hybrid quantum mechanics/molecular mechanics simulations, we propose an atomic structure for the Ag+-mediated guanine duplex with two nucleobases per strand, G2-Ag2+-G2. We then compare experimental and time-dependent density functional theory-simulated electronic circular dichroism (ECD) spectra to validate our results. Both experimental and simulated ECD share two negative peaks around 220 and 280 nm, with no positive signal in the measured wavelength range. We found that the left- or right-handed disposition of bases in the structure has a decisive effect on the signs of the ECD. We conclude that G2-Ag2+-G2 is left-hand-oriented, and extrapolation of this orientation to longer strands gives rise to a left-hand-oriented parallel helix stabilized by interplanar H bonds.

AB - Recent experimental duplexes of DNA stabilized by Ag cations, pairing homostrands of guanine-guanine, cytosine-cytosine, adenine-thymine, and thymine-thymine, display much higher stability than the Watson-Crick paired DNA duplexes; these broaden the range of applications for DNA nanotechnology. Here we focus on silver-stabilized guanine duplexes in water. Using hybrid quantum mechanics/molecular mechanics simulations, we propose an atomic structure for the Ag+-mediated guanine duplex with two nucleobases per strand, G2-Ag2+-G2. We then compare experimental and time-dependent density functional theory-simulated electronic circular dichroism (ECD) spectra to validate our results. Both experimental and simulated ECD share two negative peaks around 220 and 280 nm, with no positive signal in the measured wavelength range. We found that the left- or right-handed disposition of bases in the structure has a decisive effect on the signs of the ECD. We conclude that G2-Ag2+-G2 is left-hand-oriented, and extrapolation of this orientation to longer strands gives rise to a left-hand-oriented parallel helix stabilized by interplanar H bonds.

UR - http://www.scopus.com/inward/record.url?scp=85051831735&partnerID=8YFLogxK

U2 - 10.1021/acs.jpclett.8b01908

DO - 10.1021/acs.jpclett.8b01908

M3 - Article

VL - 9

SP - 4789

EP - 4794

JO - Journal of Physical Chemistry Letters

JF - Journal of Physical Chemistry Letters

SN - 1948-7185

IS - 16

ER -

ID: 27537767