Silica–gentamicin nanohybrids: Combating antibiotic resistance, bacterial biofilms, and in vivo toxicity
Tutkimustuotos: Lehtiartikkeli › › vertaisarvioitu
- University of Helsinki
- Animal Health Research Institute
- University of Science and Technology Beijing
- Tsinghua University
Materials and methods: In this work, we have investigated the effects of SiO2-G nanohybrids against planktonic MRSA as well as MRSA and Escherichia coli biofilms and then evaluated their toxicity in zebrafish embryos, which are an excellent model for assessing the toxicity of nanotherapeutics.
Results: SiO -G nanohybrids inhibited the growth and killed planktonic MRSA at a minimum concentration of 500 g/mL. SiO2-G nanohybrids entirely eradicated E. coli cells in biofilms at a minimum concentration of 250 g/mL and utterly deformed their ultrastructure through the deterioration of bacterial shapes and wrinkling of their cell walls. Zebrafish embryos exposed to SiO2-G nanohybrids (500 and 1,000 g/mL) showed a nonsignificant increase in mortality rates, 13.4 9.4 and 15% 7.1%, respectively, mainly detected 24 hours post fertilization (hpf). Frequencies of malformations were significantly different from the control group only 24 hpf at the higher exposure concentration.
Conclusion: Collectively, this work provides the first comprehensive in vivo assessment of SiO2-G nanohybrids as a biocompatible drug delivery system and describes the efficacy of SiO2-G nanohybrids in combating planktonic MRSA cells and eradicating E. coli biofilms.
|Julkaisu||International journal of nanomedicine|
|Tila||Julkaistu - 2018|
|OKM-julkaisutyyppi||A1 Julkaistu artikkeli, soviteltu|