Shape and Phase Transitions in a PEGylated Phospholipid System

Lauri Viitala, Saija Pajari, Luigi Gentile, Jukka Määttä, Marta Gubitosi, Jan Deska, Maria Sammalkorpi, Ulf Olsson, Lasse Murtomäki*

*Tämän työn vastaava kirjoittaja

Tutkimustuotos: LehtiartikkeliArticleScientificvertaisarvioitu

1 Sitaatiot (Scopus)
146 Lataukset (Pure)

Abstrakti

Poly(ethylene glycol) (PEG) polymers and PEG-conjugated lipids are widely used in bioengineering and drug transport applications. A PEG layer in a drug carrier increases hydrophilic repulsion, inhibits membrane fusion and serum opsonin interactions, and prolongs the storage and circulation time. It can also change the carrier shape and have an influence on many properties related to the content release of the carrier. In this paper, we focus on the physicochemical effects of PEGylation in the lipid bilayer. We introduce laurdanC as a fluorophore for shape recognition and phase transition detection. Together with laurdanC, cryogenic transmission electron microscopy, differential scanning calorimetry, molecular dynamics simulations, and small-angle X-ray scattering/wide-angle X-ray scattering, we acquire information of the particle/bilayer morphology and phase behavior in systems containing 1,2-dipalmitoyl-sn-glycero-3-phosphocholine:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-PEG(2000) with different fractions. We find that PEGylation leads to two important and potentially usable features of the system. (1) Spherical vesicles present a window of elevated chain-melting temperatures and (2) lipid packing shape-controlled liposome-to-bicelle transition. The first finding is significant for targets requiring multiple release sequences and the second enables tuning the release by composition and the PEG polymer length. Besides drug delivery systems, the findings can be used in other smart soft materials with trigger-polymers as well.

AlkuperäiskieliEnglanti
Sivut3999-4010
Sivumäärä12
JulkaisuLangmuir
Vuosikerta35
Numero11
DOI - pysyväislinkit
TilaJulkaistu - 19 maaliskuuta 2019
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

Sormenjälki Sukella tutkimusaiheisiin 'Shape and Phase Transitions in a PEGylated Phospholipid System'. Ne muodostavat yhdessä ainutlaatuisen sormenjäljen.

  • Projektit

    • 1 Päättynyt

    LADDS: Light Activated Drug Delivery System

    Murtomäki, L. & Viitala, L.

    01/09/201630/09/2018

    Projekti: Business Finland: Other research funding

    Laitteet

    OtaNano

    Anna Rissanen (Manager)

    Aalto-yliopisto

    Laitteistot/tilat: Facility

  • Siteeraa tätä

    Viitala, L., Pajari, S., Gentile, L., Määttä, J., Gubitosi, M., Deska, J., ... Murtomäki, L. (2019). Shape and Phase Transitions in a PEGylated Phospholipid System. Langmuir, 35(11), 3999-4010. https://doi.org/10.1021/acs.langmuir.8b03829