Serum Albumin-Peptide Conjugates for Simultaneous Heparin Binding and Detection

Qing Liu, Salla Välimäki, Ahmed Shaukat, Boxuan Shen, Veikko Linko, Mauri A. Kostiainen*

*Tämän työn vastaava kirjoittaja

Tutkimustuotos: LehtiartikkeliArticleScientificvertaisarvioitu

1 Sitaatiot (Scopus)
23 Lataukset (Pure)

Abstrakti

Heparin is a polysaccharide-based anticoagulant agent, which is widely used in surgery and blood transfusion. However, overdosage of heparin may cause severe side effects such as bleeding and low blood platelet count. Currently, there is only one clinically licensed antidote for heparin: Protamine sulfate, which is known to provoke adverse effects. In this work, we present a stable and biocompatible alternative for protamine sulfate that is based on serum albumin, which is conjugated with a variable number of heparin-binding peptides. The heparin-binding efficiency of the conjugates was evaluated with methylene blue displacement assay, dynamic light scattering, and anti-Xa assay. We found that multivalency of the peptides played a key role in the observed heparin-binding affinity and complex formation. The conjugates had low cytotoxicity and low hemolytic activity, indicating excellent biocompatibility. Furthermore, a sensitive DNA competition assay for heparin detection was developed. The detection limit of heparin was 0.1 IU/mL, which is well below its therapeutic range (0.2-0.4 IU/mL). Such biomolecule-based systems are urgently needed for next-generation biocompatible materials capable of simultaneous heparin binding and sensing.

AlkuperäiskieliEnglanti
Sivut21891-21899
JulkaisuACS Omega
Vuosikerta4
Numero26
DOI - pysyväislinkit
TilaJulkaistu - 2019
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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  • Projektit

    SYNVIRO: Polyelektrolyyttien Enkapsulointi Synteettisen Virologian Keinoin (SYNVIRO)

    Julin, S., Kostiainen, M., Liu, Q., Välimäki, S., Korpi, A., Ahmed, A. & Anaya Plaza, E.

    01/09/201731/08/2021

    Projekti: Academy of Finland: Other research funding

    Proteiinihäkeillä suunnattu itsejärjestyminen nanomateriaaleissa

    Ora, A., Kostiainen, M., Korpi, A., Damania, A. & Liljeström, V.

    01/09/201621/09/2018

    Projekti: Academy of Finland: Other research funding

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