Screening for novel laccase-producing microbes

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

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Screening for novel laccase-producing microbes. / Kiiskinen, L. L.; Rättö, M.; Kruus, K.

julkaisussa: Journal of Applied Microbiology, Vuosikerta 97, Nro 3, 22.09.2004, s. 640-646.

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

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Kiiskinen, L. L. ; Rättö, M. ; Kruus, K. / Screening for novel laccase-producing microbes. Julkaisussa: Journal of Applied Microbiology. 2004 ; Vuosikerta 97, Nro 3. Sivut 640-646.

Bibtex - Lataa

@article{65bdb30a45684c909558a65423f7ee1c,
title = "Screening for novel laccase-producing microbes",
abstract = "Aims: To discover novel laccases potential for industrial applications. Methods and Results: Fungi were cultivated on solid media containing indicator compounds that enabled the detection of laccases as specific colour reactions. The indicators used were Remazol Brilliant Blue R (RBBR), Poly R-478, guaiacol and tannic acid. The screening work resulted in isolation of 26 positive fungal strains. Liquid cultivations of positive strains confirmed that four efficient laccase producers were found in the screening. Biochemical characteristics of the four novel laccases were typical for fungal laccases in terms of molecular weight, pH optima and pI. The laccases showed good thermal stability at 60°C. Conclusions: Plate-test screening based on polymeric dye compounds, guaiacol and tannic acid is an efficient way to discover novel laccase producers. The results indicated that screening for laccase activity can be performed with guaiacol and RBBR or Poly R-478. Significance and Impact of the Study: Laccases have many potential industrial applications including textile dye decolourization, delignification of pulp and effluent detoxification. It is essential to find novel, efficient enzymes to further develop these applications. This study showed that relatively simple plate test screening method can be used for discovery of novel laccases.",
keywords = "Guaiacol, Laccase, Poly R-478, RBBR, Screening, Tannic acid",
author = "Kiiskinen, {L. L.} and M. R{\"a}tt{\"o} and K. Kruus",
year = "2004",
month = "9",
day = "22",
doi = "10.1111/j.1365-2672.2004.02348.x",
language = "English",
volume = "97",
pages = "640--646",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley",
number = "3",

}

RIS - Lataa

TY - JOUR

T1 - Screening for novel laccase-producing microbes

AU - Kiiskinen, L. L.

AU - Rättö, M.

AU - Kruus, K.

PY - 2004/9/22

Y1 - 2004/9/22

N2 - Aims: To discover novel laccases potential for industrial applications. Methods and Results: Fungi were cultivated on solid media containing indicator compounds that enabled the detection of laccases as specific colour reactions. The indicators used were Remazol Brilliant Blue R (RBBR), Poly R-478, guaiacol and tannic acid. The screening work resulted in isolation of 26 positive fungal strains. Liquid cultivations of positive strains confirmed that four efficient laccase producers were found in the screening. Biochemical characteristics of the four novel laccases were typical for fungal laccases in terms of molecular weight, pH optima and pI. The laccases showed good thermal stability at 60°C. Conclusions: Plate-test screening based on polymeric dye compounds, guaiacol and tannic acid is an efficient way to discover novel laccase producers. The results indicated that screening for laccase activity can be performed with guaiacol and RBBR or Poly R-478. Significance and Impact of the Study: Laccases have many potential industrial applications including textile dye decolourization, delignification of pulp and effluent detoxification. It is essential to find novel, efficient enzymes to further develop these applications. This study showed that relatively simple plate test screening method can be used for discovery of novel laccases.

AB - Aims: To discover novel laccases potential for industrial applications. Methods and Results: Fungi were cultivated on solid media containing indicator compounds that enabled the detection of laccases as specific colour reactions. The indicators used were Remazol Brilliant Blue R (RBBR), Poly R-478, guaiacol and tannic acid. The screening work resulted in isolation of 26 positive fungal strains. Liquid cultivations of positive strains confirmed that four efficient laccase producers were found in the screening. Biochemical characteristics of the four novel laccases were typical for fungal laccases in terms of molecular weight, pH optima and pI. The laccases showed good thermal stability at 60°C. Conclusions: Plate-test screening based on polymeric dye compounds, guaiacol and tannic acid is an efficient way to discover novel laccase producers. The results indicated that screening for laccase activity can be performed with guaiacol and RBBR or Poly R-478. Significance and Impact of the Study: Laccases have many potential industrial applications including textile dye decolourization, delignification of pulp and effluent detoxification. It is essential to find novel, efficient enzymes to further develop these applications. This study showed that relatively simple plate test screening method can be used for discovery of novel laccases.

KW - Guaiacol

KW - Laccase

KW - Poly R-478

KW - RBBR

KW - Screening

KW - Tannic acid

UR - http://www.scopus.com/inward/record.url?scp=4444306249&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2672.2004.02348.x

DO - 10.1111/j.1365-2672.2004.02348.x

M3 - Article

VL - 97

SP - 640

EP - 646

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 3

ER -

ID: 32971200