SACCHAROMYCES-CEREVISIAE CELLS SECRETING AN ASPERGILLUS-NIGER BETA-GALACTOSIDASE GROW ON WHEY PERMEATE

Pardeep KUMAR, S RAMAKRISHNAN, Tuula T. Teeri, JKC KNOWLES, BS HARTLEY

Tutkimustuotos: LehtiartikkeliArticleScientificvertaisarvioitu

Abstrakti

We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermo-stable beta-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total beta-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.

AlkuperäiskieliEnglanti
Sivut82-85
Sivumäärä4
JulkaisuBio-Technology
Vuosikerta10
Numero1
TilaJulkaistu - tammikuuta 1992
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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