Resolving host-guest interactions between pillararenes and homoserine lactones to restrain bacterial quorum sensing

Tutkimustuotos: LehtiartikkeliArticleScientificvertaisarvioitu

1 Sitaatiot (Scopus)
24 Lataukset (Pure)

Abstrakti

Using supramolecular host molecules to hinder bacterial quorum sensing (QS) is a potential approach in circumventing antimicrobial resistance (AMR). The emergent family of pillararenes offers promising candidates for binding homoserine lactones (HSLs), signaling molecules used by gram-negative species, including WHO critical-priority antibiotic-resistant bacteria. Here, we compare seven cationic pillararenes against four HSLs, from (supra)molecular interactions to biological assays. Complexation, characterized by dye displacement assay and NMR spectroscopy, complemented by all-atom molecular dynamics (MD) simulations, was compared to effects in biological systems, studied using a bacterial HSL reporter system as well as biofilm and pyocyanin assays as models of QS-mediated virulence. HSL binding improves approximately 10-fold versus previous reports with a hydroxyl-functionalized pillararene, and a deeper-cavity host with marked preference for the longest-tailed HSL is identified. Successful HSL capture is directly reflected as impaired biofilm formation and pyocyanin production and improved healing in open wound in vivo models.

AlkuperäiskieliEnglanti
Artikkeli102089
Sivumäärä19
JulkaisuCell Reports Physical Science
Vuosikerta5
Numero7
DOI - pysyväislinkit
TilaJulkaistu - 17 heinäk. 2024
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

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