Reconfigurable DNA Origami Nanocapsule for pH-Controlled Encapsulation and Display of Cargo

Heini Ijäs, Iiris Hakaste, Boxuan Shen, Mauri A. Kostiainen, Veikko Linko*

*Tämän työn vastaava kirjoittaja

Tutkimustuotos: LehtiartikkeliArticleScientificvertaisarvioitu

53 Sitaatiot (Scopus)
247 Lataukset (Pure)

Abstrakti

DNA nanotechnology provides a toolbox for creating custom and precise nanostructures with nanometer-level accuracy. These nano-objects are often static by nature and serve as versatile templates for assembling various molecular components in a user-defined way. In addition to the static structures, the intrinsic programmability of DNA nanostructures allows the design of dynamic devices that can perform predefined tasks when triggered with external stimuli, such as drug delivery vehicles whose cargo display or release can be triggered with a specified physical or chemical cue in the biological environment. Here, we present a DNA origami nanocapsule that can be loaded with cargo and reversibly opened and closed by changing the pH of the surrounding solution. Moreover, the threshold pH value for opening/closing can be rationally designed. We characterize the reversible switching and a rapid opening of "pH-latch"-equipped nanocapsules using Förster resonance energy transfer. Furthermore, we demonstrate the full cycle of capsule loading, encapsulation, and displaying the payload using metal nanoparticles and functional enzymes as cargo mimics at physiologically relevant ion concentrations.

AlkuperäiskieliEnglanti
Sivut5959-5967
Sivumäärä9
JulkaisuACS Nano
Vuosikerta13
Numero5
DOI - pysyväislinkit
TilaJulkaistu - 28 toukokuuta 2019
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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