TY - JOUR
T1 - Protein Adsorption and Coordination-Based End-Tethering of Functional Polymers on Metal-Phenolic Network Films
AU - Tardy, Blaise L.
AU - Richardson, Joseph J.
AU - Nithipipat, Vichida
AU - Kempe, Kristian
AU - Guo, Junling
AU - Cho, Kwun Lun
AU - Rahim, Md Arifur
AU - Ejima, Hirotaka
AU - Caruso, Frank
PY - 2019/3/11
Y1 - 2019/3/11
N2 -
Metal-phenolic network (MPN) coatings have generated increasing interest owing to their biologically inspired nature, facile fabrication, and near-universal adherence, especially for biomedical applications. However, a key issue in biomedicine is protein fouling, and the adsorption of proteins on tannic acid-based MPNs remains to be comprehensively studied. Herein, we investigate the interaction of specific biomedically relevant proteins in solution (e.g., bovine serum albumin (BSA), immunoglobulin G (IgG), fibrinogen) and complex biological media (serum) using layer-by-layer-assembled tannic acid/Fe
III
MPN films. When Fe
III
was the outermost layer, galloyl-modified poly(2-ethyl-2-oxazoline) (P(EtOx)-Gal) could be grafted to the films through coordination bonds. Protein fouling and bacterial adhesion were greatly suppressed after functionalization with P(EtOx)-Gal and the mass of adsorbed protein was reduced by 79%. Interestingly, larger proteins adsorbed more on both the MPNs and P(EtOx)-functionalized MPNs. This study provides fundamental information on the interactions of MPNs with single proteins, mixtures of proteins as encountered in serum, and the noncovalent, coordination-based, functionalization of MPN films.
AB -
Metal-phenolic network (MPN) coatings have generated increasing interest owing to their biologically inspired nature, facile fabrication, and near-universal adherence, especially for biomedical applications. However, a key issue in biomedicine is protein fouling, and the adsorption of proteins on tannic acid-based MPNs remains to be comprehensively studied. Herein, we investigate the interaction of specific biomedically relevant proteins in solution (e.g., bovine serum albumin (BSA), immunoglobulin G (IgG), fibrinogen) and complex biological media (serum) using layer-by-layer-assembled tannic acid/Fe
III
MPN films. When Fe
III
was the outermost layer, galloyl-modified poly(2-ethyl-2-oxazoline) (P(EtOx)-Gal) could be grafted to the films through coordination bonds. Protein fouling and bacterial adhesion were greatly suppressed after functionalization with P(EtOx)-Gal and the mass of adsorbed protein was reduced by 79%. Interestingly, larger proteins adsorbed more on both the MPNs and P(EtOx)-functionalized MPNs. This study provides fundamental information on the interactions of MPNs with single proteins, mixtures of proteins as encountered in serum, and the noncovalent, coordination-based, functionalization of MPN films.
UR - http://www.scopus.com/inward/record.url?scp=85062690872&partnerID=8YFLogxK
U2 - 10.1021/acs.biomac.9b00006
DO - 10.1021/acs.biomac.9b00006
M3 - Article
C2 - 30794387
AN - SCOPUS:85062690872
VL - 20
SP - 1421
EP - 1428
JO - Biomacromolecules
JF - Biomacromolecules
SN - 1525-7797
IS - 3
ER -