Preparation and Characterization of Dentin Phosphophoryn-Derived Peptide-Functionalized Lignin Nanoparticles for Enhanced Cellular Uptake

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

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Preparation and Characterization of Dentin Phosphophoryn-Derived Peptide-Functionalized Lignin Nanoparticles for Enhanced Cellular Uptake. / Figueiredo, Patrícia; Sipponen, Mika H.; Lintinen, Kalle; Correia, Alexandra; Kiriazis, Alexandros; Yli-Kauhaluoma, Jari; Österberg, Monika; George, Anne; Hirvonen, Jouni; Kostiainen, Mauri A.; Santos, Hélder A.

julkaisussa: Small, Vuosikerta 15, Nro 24, 1901427, 14.06.2019.

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Harvard

Figueiredo, P, Sipponen, MH, Lintinen, K, Correia, A, Kiriazis, A, Yli-Kauhaluoma, J, Österberg, M, George, A, Hirvonen, J, Kostiainen, MA & Santos, HA 2019, 'Preparation and Characterization of Dentin Phosphophoryn-Derived Peptide-Functionalized Lignin Nanoparticles for Enhanced Cellular Uptake' Small, Vuosikerta. 15, Nro 24, 1901427. https://doi.org/10.1002/smll.201901427

APA

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Author

Figueiredo, Patrícia ; Sipponen, Mika H. ; Lintinen, Kalle ; Correia, Alexandra ; Kiriazis, Alexandros ; Yli-Kauhaluoma, Jari ; Österberg, Monika ; George, Anne ; Hirvonen, Jouni ; Kostiainen, Mauri A. ; Santos, Hélder A. / Preparation and Characterization of Dentin Phosphophoryn-Derived Peptide-Functionalized Lignin Nanoparticles for Enhanced Cellular Uptake. Julkaisussa: Small. 2019 ; Vuosikerta 15, Nro 24.

Bibtex - Lataa

@article{616b4b52d94a4ea7803ab4d29e94cc9f,
title = "Preparation and Characterization of Dentin Phosphophoryn-Derived Peptide-Functionalized Lignin Nanoparticles for Enhanced Cellular Uptake",
abstract = "The surface modification of nanoparticles (NPs) using different ligands is a common strategy to increase NP−cell interactions. Here, dentin phosphophoryn-derived peptide (DSS) lignin nanoparticles (LNPs) are prepared and characterized, the cellular internalization of the DSS-functionalized LNPs (LNPs-DSS) into three different cancer cell lines is evaluated, and their efficacy with the widely used iRGD peptide is compared. It is shown that controlled extent of carboxylation of lignin improves the stability at physiological conditions of LNPs formed upon solvent exchange. Functionalization with DSS and iRGD peptides maintains the spherical morphology and moderate polydispersity of LNPs. The LNPs exhibit good cytocompatibility when cultured with PC3-MM2, MDA-MB-231, and A549 in the conventional 2D model and in the 3D cell spheroid morphology. Importantly, the 3D cell models reveal augmented internalization of peptide-functionalized LNPs and improve antiproliferative effects when the LNPs are loaded with a cytotoxic compound. Overall, LNPs-DSS show equal or even superior cellular internalization than the LNPs-iRGD, suggesting that DSS can also be used to enhance the cellular uptake of NPs into different types of cells, and release different cargos intracellularly.",
keywords = "3D cell culture, benzazulene, biofunctionalization, dentin phosphophoryn peptide, lignin nanoparticles",
author = "Patr{\'i}cia Figueiredo and Sipponen, {Mika H.} and Kalle Lintinen and Alexandra Correia and Alexandros Kiriazis and Jari Yli-Kauhaluoma and Monika {\"O}sterberg and Anne George and Jouni Hirvonen and Kostiainen, {Mauri A.} and Santos, {H{\'e}lder A.}",
year = "2019",
month = "6",
day = "14",
doi = "10.1002/smll.201901427",
language = "English",
volume = "15",
journal = "Small",
issn = "1613-6810",
number = "24",

}

RIS - Lataa

TY - JOUR

T1 - Preparation and Characterization of Dentin Phosphophoryn-Derived Peptide-Functionalized Lignin Nanoparticles for Enhanced Cellular Uptake

AU - Figueiredo, Patrícia

AU - Sipponen, Mika H.

AU - Lintinen, Kalle

AU - Correia, Alexandra

AU - Kiriazis, Alexandros

AU - Yli-Kauhaluoma, Jari

AU - Österberg, Monika

AU - George, Anne

AU - Hirvonen, Jouni

AU - Kostiainen, Mauri A.

AU - Santos, Hélder A.

PY - 2019/6/14

Y1 - 2019/6/14

N2 - The surface modification of nanoparticles (NPs) using different ligands is a common strategy to increase NP−cell interactions. Here, dentin phosphophoryn-derived peptide (DSS) lignin nanoparticles (LNPs) are prepared and characterized, the cellular internalization of the DSS-functionalized LNPs (LNPs-DSS) into three different cancer cell lines is evaluated, and their efficacy with the widely used iRGD peptide is compared. It is shown that controlled extent of carboxylation of lignin improves the stability at physiological conditions of LNPs formed upon solvent exchange. Functionalization with DSS and iRGD peptides maintains the spherical morphology and moderate polydispersity of LNPs. The LNPs exhibit good cytocompatibility when cultured with PC3-MM2, MDA-MB-231, and A549 in the conventional 2D model and in the 3D cell spheroid morphology. Importantly, the 3D cell models reveal augmented internalization of peptide-functionalized LNPs and improve antiproliferative effects when the LNPs are loaded with a cytotoxic compound. Overall, LNPs-DSS show equal or even superior cellular internalization than the LNPs-iRGD, suggesting that DSS can also be used to enhance the cellular uptake of NPs into different types of cells, and release different cargos intracellularly.

AB - The surface modification of nanoparticles (NPs) using different ligands is a common strategy to increase NP−cell interactions. Here, dentin phosphophoryn-derived peptide (DSS) lignin nanoparticles (LNPs) are prepared and characterized, the cellular internalization of the DSS-functionalized LNPs (LNPs-DSS) into three different cancer cell lines is evaluated, and their efficacy with the widely used iRGD peptide is compared. It is shown that controlled extent of carboxylation of lignin improves the stability at physiological conditions of LNPs formed upon solvent exchange. Functionalization with DSS and iRGD peptides maintains the spherical morphology and moderate polydispersity of LNPs. The LNPs exhibit good cytocompatibility when cultured with PC3-MM2, MDA-MB-231, and A549 in the conventional 2D model and in the 3D cell spheroid morphology. Importantly, the 3D cell models reveal augmented internalization of peptide-functionalized LNPs and improve antiproliferative effects when the LNPs are loaded with a cytotoxic compound. Overall, LNPs-DSS show equal or even superior cellular internalization than the LNPs-iRGD, suggesting that DSS can also be used to enhance the cellular uptake of NPs into different types of cells, and release different cargos intracellularly.

KW - 3D cell culture

KW - benzazulene

KW - biofunctionalization

KW - dentin phosphophoryn peptide

KW - lignin nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=85065435965&partnerID=8YFLogxK

U2 - 10.1002/smll.201901427

DO - 10.1002/smll.201901427

M3 - Article

VL - 15

JO - Small

JF - Small

SN - 1613-6810

IS - 24

M1 - 1901427

ER -

ID: 33943955