TY - JOUR
T1 - Performance and bacterial community dynamics of a CANON bioreactor acclimated from high to low operational temperatures
AU - Gonzalez-Martinez, Alejandro
AU - Rodriguez-Sanchez, Alejandro
AU - Jesus Garcia-Ruiz, Maria
AU - Munoz-Palazon, Babara
AU - Cortes-Lorenzo, Carmen
AU - Osorio, Franscisco
AU - Vahala, Riku
PY - 2016/3/1
Y1 - 2016/3/1
N2 - In this study, a lab-scale CANON bioreactor was operated for 260 days, decreasing operational temperature from 35 degrees C to 15 degrees C and from 466 to 100 mg-N L-1 ammonium. This was done in order to check the feasibility of the acclimation of CANON biomass treating anaerobic digestion supernatant to B-stage influent wastewater operational conditions. Results showed that decrease in temperature posed an impact over the performance of the CANON bioreactor as well as over its bacterial assemblage. Nitrogen removal efficiency showed a moderate decrease when the system was acclimated from 35 degrees C to 25 degrees C, but it decreased dramatically in the acclimation from 25 degrees C to 15 degrees C. The decrease in temperature and influent ammonium concentration posed an impact over the bacterial community structure of the system. Ammonium oxidizing bacteria changed from Nitrosomonas, Nitrosospira or Nitrosovibrio genera at high temperature and influent ammonium to Prosthecobacter at low temperature and low ammonium. As well, dominant anammox bacteria genus changed from Candidatus Brocadia to Candidatus Anammoxoglobus during acclimation. Proliferation of nitrite oxidizing bacteria only occurred under low temperature and influent ammonium conditions with growth of Rhodanobacter genus. (C) 2015 Elsevier B.V. All rights reserved.
AB - In this study, a lab-scale CANON bioreactor was operated for 260 days, decreasing operational temperature from 35 degrees C to 15 degrees C and from 466 to 100 mg-N L-1 ammonium. This was done in order to check the feasibility of the acclimation of CANON biomass treating anaerobic digestion supernatant to B-stage influent wastewater operational conditions. Results showed that decrease in temperature posed an impact over the performance of the CANON bioreactor as well as over its bacterial assemblage. Nitrogen removal efficiency showed a moderate decrease when the system was acclimated from 35 degrees C to 25 degrees C, but it decreased dramatically in the acclimation from 25 degrees C to 15 degrees C. The decrease in temperature and influent ammonium concentration posed an impact over the bacterial community structure of the system. Ammonium oxidizing bacteria changed from Nitrosomonas, Nitrosospira or Nitrosovibrio genera at high temperature and influent ammonium to Prosthecobacter at low temperature and low ammonium. As well, dominant anammox bacteria genus changed from Candidatus Brocadia to Candidatus Anammoxoglobus during acclimation. Proliferation of nitrite oxidizing bacteria only occurred under low temperature and influent ammonium conditions with growth of Rhodanobacter genus. (C) 2015 Elsevier B.V. All rights reserved.
KW - Anammox
KW - CANON
KW - Low temperature
KW - Nitrogen removal
KW - Pyrosequencing
KW - Wastewater treatment
KW - AUTOTROPHIC NITROGEN-REMOVAL
KW - WASTE-WATER
KW - SP-NOV.
KW - PARTIAL NITRITATION/ANAMMOX
KW - MEMBRANE BIOREACTOR
KW - MICROBIAL COMMUNITY
KW - OXIDIZING BACTERIA
KW - LAB-SCALE
KW - SYSTEM
KW - NITRIFICATION
U2 - 10.1016/j.cej.2015.11.081
DO - 10.1016/j.cej.2015.11.081
M3 - Article
SN - 1385-8947
VL - 287
SP - 557
EP - 567
JO - Chemical Engineering Journal
JF - Chemical Engineering Journal
ER -