Pathway and morphological transformation of liposome nanocarriers after release from a novel sustained inner-ear delivery system

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Tutkijat

  • Jing Zou
  • Rohit Sood
  • Ya Zhang
  • Paavo K J Kinnunen
  • Ilmari Pyykkö

Organisaatiot

  • Second Military Medical University
  • Tampere University

Kuvaus

Aim: To validate a novel sustained delivery system of liposome nanocarriers for inner-ear therapy and to investigate the transport pathway for their delivery. Materials & methods: Liposome nanocarriers containing gadolinium-tetra-azacyclo-dodecane-tetra-acetic acid (LPS+Gd-DOTA) were developed for MRI tracking the in vitro release profile and for in vivo uptake studies. Results: Encapsulating Gd-DOTA did not modify the liposomes. The LPS+Gd-DOTA nanocarriers were slowly released from a miniature osmotic pump. The LPS+Gd-DOTA moved along the ossicular chain toward the oval window after an epitympanic injection, whereas they traveled directly to the round window after a mesotympanic injection. However, the round window membrane was the major pathway for the LPS+Gd-DOTA to enter the inner ear. LPS+Gd-DOTA were visualized on both sides of the cochlea within 6 days of in vivo delivery via the osmotic pump. Discussion: The novel sustained inner-ear delivery system induced liposome nanocarriers into the inner ear efficiently without causing obvious adverse effect. There is the potential of using the system to administrate therapeutics in treating inner-ear diseases in the clinic.

Yksityiskohdat

AlkuperäiskieliEnglanti
Sivut2143-2155
Sivumäärä13
JulkaisuNanomedicine
Vuosikerta9
Numero14
TilaJulkaistu - 1 lokakuuta 2014
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

ID: 9431657