Packaging DNA origami into viral protein cages

Veikko Linko, Joona Mikkilä, Mauri A. Kostiainen*

*Tämän työn vastaava kirjoittaja

Tutkimustuotos: Artikkeli kirjassa/konferenssijulkaisussaChapterScientificvertaisarvioitu

3 Sitaatiot (Scopus)
308 Lataukset (Pure)

Abstrakti

The DNA origami technique is a widely used method to create customized, complex, spatially well-defined two-dimensional (2D) and three-dimensional (3D) DNA nanostructures. These structures have huge potential to serve as smart drug-delivery vehicles and molecular devices in various nanomedical and biotechnological applications. However, so far only little is known about the behavior of these novel structures in living organisms or in cell culture/tissue models. Moreover, enhancing pharmacokinetic bioavailability and transfection properties of such structures still remains a challenge. One intriguing approach to overcome these issues is to coat DNA origami nanostructures with proteins or lipid membranes. Here, we show how cowpea chlorotic mottle virus (CCMV) capsid proteins (CPs) can be used for coating DNA origami nanostructures. We present a method for disassembling native CCMV particles and isolating the pure CP dimers, which can further bind and encapsulate a rectangular DNA origami shape. Owing to the highly programmable nature of DNA origami, packaging of DNA nanostructures into viral protein cages could find imminent uses in enhanced targeting and cellular delivery of various active nano-objects, such as enzymes and drug molecules.

AlkuperäiskieliEnglanti
OtsikkoVirus-Derived Nanoparticles for Advanced Technologies
ToimittajatChristina Wege, George P. Lomonossoff
KustantajaSpringer
Sivut267-277
Sivumäärä11
Vuosikerta1776
ISBN (elektroninen)978-1-4939-7808-3
ISBN (painettu)978-1-4939-7806-9
DOI - pysyväislinkit
TilaJulkaistu - 1 tammik. 2018
OKM-julkaisutyyppiA3 Kirjan tai muun kokoomateoksen osa

Julkaisusarja

NimiMethods in Molecular Biology
Vuosikerta1776
ISSN (painettu)1064-3745

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