On-line measurement of the substrate concentrations in Pichia pastoris fermentations using FT-IR/ATR

John Dahlbacka*, Jan Weegar, Niklas von Weymarn, Kaj Fagervik

*Tämän työn vastaava kirjoittaja

Tutkimustuotos: LehtiartikkeliArticleScientificvertaisarvioitu

13 Sitaatiot (Scopus)

Abstrakti

The glycerol and methanol concentrations in Pichia pastoris fermentations were measured on-line using Fourier transform infrared spectroscopy and an attenuated total reflection probe. Partial least squares regression was used to obtain calibration models. The models were regressed on synthetic multi-component spectra and semi-synthetic fermentation broth spectra. These were obtained by spectral addition. The accuracy for the on-line measurement of glycerol, given as standard error of prediction (SEP), was determined to 0.68 g/l, and the SEP of methanol was 0.13 g/l. We show how reliable calibration models are obtained relatively effortlessly by replacing extensive sampling from the reactor with simple mathematical manipulations of the model regression spectra.

AlkuperäiskieliEnglanti
Sivut1009-1017
Sivumäärä9
JulkaisuBiotechnology Letters
Vuosikerta34
Numero6
DOI - pysyväislinkit
TilaJulkaistu - kesäk. 2012
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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