Observation of in vitro cellulose synthesis by bacterial cellulose synthase with time-resolved small angle X-ray scattering

Hirotaka Tajima, Paavo A. Penttilä, Tomoya Imai*, Kyoko Yamamoto, Yoshiaki Yuguchi

*Tämän työn vastaava kirjoittaja

Tutkimustuotos: LehtiartikkeliArticleScientificvertaisarvioitu

8 Sitaatiot (Scopus)

Abstrakti

Cellulose synthase is the enzyme that produces cellulose in the living organisms like plant, and has two functions: polymerizing glucose residues (polymerization) and assembling these polymerized molecules into a crystalline microfibril with a “cellulose I” crystallographic structure (crystallization). Many studies, however, have shown that an in vitro reaction of cellulose synthase produces aggregates of a non-native crystallographic structure “cellulose II” despite the remaining polymerizing activity. This is partial denaturation or loss of crystallization function in cellulose synthase, which needs to be resolved to reconstitute its native activity. To this end, we aimed to clarify the process of cellulose II formation by bacterial cellulose synthase in vitro, using in situ small angle X-ray scattering (SAXS). An increase in scattering specific to synthesis was observed around two distinct regions of q (0.2–0.4 nm −1 and <0.1 nm −1 ) by time-resolved SAXS measurement. The scattering at higher q-region appears prior to lower-q scattering at beginning of the reaction, indicating the existence of smaller primitive aggregations at the initiation stage. This study demonstrates the use of in situ SAXS measurement to decipher the dynamics of biosynthesized cellulose chains, which is a remarkable example of polymer assembly in ambient conditions.

AlkuperäiskieliEnglanti
Sivut765-777
Sivumäärä13
JulkaisuInternational Journal of Biological Macromolecules
Vuosikerta130
DOI - pysyväislinkit
TilaJulkaistu - 1 kesäk. 2019
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Sormenjälki

Sukella tutkimusaiheisiin 'Observation of in vitro cellulose synthesis by bacterial cellulose synthase with time-resolved small angle X-ray scattering'. Ne muodostavat yhdessä ainutlaatuisen sormenjäljen.

Siteeraa tätä