Nanoscale Assembly Using DNA and Electromagnetic Fields

Antti-Pekka Eskelinen

Nanoscale Assembly Using DNA and Electromagnetic Fields

Julkaisun otsikon käännös: Nanoscale Assembly Using DNA and Electromagnetic Fields

Antti-Pekka Eskelinen

Teknillisen fysiikan laitos

Tutkimustuotos: Doctoral Thesis › Collection of Articles

Abstrakti

Tämä väitöskirja käsittelee nanohiukkasten ja nanorakenteiden kontrollointia nanomittakaavassa DNA-origami-tekniikan sekä dielektroforeesin avulla. DNA-origami-tekniikka on DNA-nanoteknologian viimeaikaisimpia saavutuksia, jossa DNA:a käytetään geneettisen informaation säilömisen sijaan rakennusmateriaalina. Tässä väitöskirjassa DNA-rakenteita käytetään etenkin erilaisten nanopartikkelien kiinnittämiseen ja asemoimiseen. Ensimmäisenä DNA-origamien sovellutuskohteena osoitamme menetelmän yksiseinäisten hiilinanoputkien kontrolloituun asemoimiseen. Osoitamme lähestymistavan tehokkuuden muun muassa rakentamalla ristiliitoksen kahdesta yksiseinäisestä hiilinanoputkesta - rakenteen, jota voi käyttää hiilinanoputkitransistorina. Vastaavanlaisia rakenteita on erittäin vaikea tuottaa kontrolloidusti perinteisillä menetelmillä, mikä on ollut yksi este hiilinanoputkiin perustuvien laitteiden kaupallistamisessa. Hiilinanoputkien asemoinnin lisäksi sovellamme DNA-origami-tekniikkaa antennirakenteen kokoamiseen hopeananohiukkasista. Todistamme rakenteiden syntymisen atomivoima- sekä läpäisyelektronimikroskoopin avulla. Varmennamme tuloksemme myös geelielektroforeesin avulla. Yhtenä antennirakenteemme sovellutuksena voi olla DNA-anturi; tutkimme myös rakenteemme soveltuvuutta kyseiseen tarkoitukseen numeeristen simulaatioiden avulla. Viimeisenä DNA-origami-tekniikkaan liittyvänä tutkimuksena osoitamme, kuinka DNA-rakennetta itseään voidaan kontrolloida dendronien sekä ulkoisten signaalien avulla. Osoitamme atomivoimamikroskooppi-, geelielektroforeesi- sekä dynaamisten valonsirontakokeiden avulla pystyvämme tehokkaasti kontrolloimaan DNA-rakenteiden muodostumisen. Kontrollisignaalit määräytyvät käytettyjen dendronirakenteiden mukaan. Kokeissamme kontrollisignaaleja ovat ultraviolettivalo sekä mieto redusoiva aine dithiothretoil. Dielektroforeesi on elektromekaaninen menetelmä, jonka avulla voi kontrolloida mikro- sekä nanokokoluokan hiukkasia sähkökenttien gradienttien avulla. Käytämme metodia uuden nanopainamismenetelmän kehittämiseen. Menetelmän pääkomponentti on leimasin, johon kuvioimme nanoelektrodit. Käytämme näitä elektrodeja muodostamaan haluttuja kuvioita nanohiukkasista sähkökenttien gradienttien avulla. Lopulta siirrämme kyseisen kuvion halutulle alustalle mekaanisen painamisen avulla. Nanopainamismenetelmän lisäksi käytämme dielektroforeesia muodostaaksemme nanohiukkasketjuja kultananohiukkasista mikro- sekä nanoelektrodien avulla. Teemme myös impedanssimittauksia kyseisillä ketjuilla anturisovellutusta varten.

Julkaisun otsikon käännös	Nanoscale Assembly Using DNA and Electromagnetic Fields
Alkuperäiskieli	Englanti
Pätevyys	Tohtorintutkinto
Myöntävä instituutio	Aalto-yliopisto
Valvoja/neuvonantaja	Törmä, Päivi, Vastuuprofessori Ikkala, Olli, Ohjaaja Kostiainen, Mauri, Ohjaaja
Kustantaja	Aalto University
Painoksen ISBN	978-952-60-5320-2
Sähköinen ISBN	978-952-60-5321-9
Tila	Julkaistu - 2013
OKM-julkaisutyyppi	G5 Artikkeliväitöskirja

Tutkimusalat

DNA-nanoteknologia
DNA origami
elektromekaniikka
nano-optiikka

Pääsy asiakirjaan

http://urn.fi/URN:ISBN:978-952-60-5321-9

Siteeraa tätä

@phdthesis{457c30f3d1b14ee1aa03130232c2bf67,

title = "Nanoscale Assembly Using DNA and Electromagnetic Fields",

abstract = "In this work we demonstrate the control of nanoparticles and nanostructures with the help of the DNA origami method and dielectrophoresis. DNA nanotechnology is a subﬁeld of nanotechnology where DNA is used as a construction material. The DNA origami method is a recent development in the ﬁeld which enables the assembly of nanoparticles with nanometer scale accuracy through self-assembly. Here we take advantage of the method for efﬁcient deposition and alignment of single-walled carbon nanotubes (SWCNTs). Especially the alignment of SWCNTs on substrates has been a major challenge for commercialization of SWCNT based devices, to which our approach could offer a potential solution. As an example, a crossed carbon nanotube junction, a basic geometry for a carbon nanotube transistor, is constructed. The high yields of assembled structures as well as correct alignment of SWCNTs are veriﬁed with atomic force microscopy. The DNA origami method is used also for assembling a bow-tie antenna conﬁguration from silver nanoparticles. The optical response of the system is based on the hybridization of the individual nanoparticle surface plasmon resonance modes. The formation of the structures is veriﬁed with transmission electron microscopy and complemented with agarose gel electrophoresis. The conﬁguration could have potential use as an optical DNA sensor. The sensor performance is investigated with ﬁnite-difference time-domain numerical simulations. In addition to assembling nanoparticles, control over the DNA origami structure itself is demonstrated with dendrons and external trigger signals. Efﬁcient control over the structure formation is investigated with atomic force microscopy, agarose gel electrophoresis and dynamic light scattering experiments. The external trigger signals can be chosen by choosing the dendron structure. We demonstrate our concept with dendrons which can be triggered either with UV-radiation or with a mild reducing agent such as dithiothretoil. Dielectrophoresis is an electromechanical technique for manipulating micro-and nanoparticles. Here the method is used for developing a new nanoimprint lithographic technique named ﬁeld-induced nanoimprint lithography. In this technique nanoelectrodes are used for producing dielectrophoretically a pattern of nanoparticles on a re-usable master stamp, which is then used for transferring the nanoparticle pattern on a target substrate by mechanical contact. Dielectrophoresis is also used for producing chains of gold nanoparticles between nano- and microelectrodes. The chains are investigated for sensing purposes with impedance studies.",

keywords = "DNA nanotechnology, DNA origami, electromechanics, nano-optics, DNA-nanoteknologia, DNA origami, elektromekaniikka, nano-optiikka, DNA nanotechnology, DNA origami, electromechanics, nano-optics",

author = "Antti-Pekka Eskelinen",

year = "2013",

language = "English",

isbn = "978-952-60-5320-2",

series = "Aalto University publication series DOCTORAL DISSERTATIONS",

publisher = "Aalto University",

number = "139",

address = "Finland",

school = "Aalto University",

}

TY - BOOK

T1 - Nanoscale Assembly Using DNA and Electromagnetic Fields

AU - Eskelinen, Antti-Pekka

PY - 2013

Y1 - 2013

N2 - In this work we demonstrate the control of nanoparticles and nanostructures with the help of the DNA origami method and dielectrophoresis. DNA nanotechnology is a subﬁeld of nanotechnology where DNA is used as a construction material. The DNA origami method is a recent development in the ﬁeld which enables the assembly of nanoparticles with nanometer scale accuracy through self-assembly. Here we take advantage of the method for efﬁcient deposition and alignment of single-walled carbon nanotubes (SWCNTs). Especially the alignment of SWCNTs on substrates has been a major challenge for commercialization of SWCNT based devices, to which our approach could offer a potential solution. As an example, a crossed carbon nanotube junction, a basic geometry for a carbon nanotube transistor, is constructed. The high yields of assembled structures as well as correct alignment of SWCNTs are veriﬁed with atomic force microscopy. The DNA origami method is used also for assembling a bow-tie antenna conﬁguration from silver nanoparticles. The optical response of the system is based on the hybridization of the individual nanoparticle surface plasmon resonance modes. The formation of the structures is veriﬁed with transmission electron microscopy and complemented with agarose gel electrophoresis. The conﬁguration could have potential use as an optical DNA sensor. The sensor performance is investigated with ﬁnite-difference time-domain numerical simulations. In addition to assembling nanoparticles, control over the DNA origami structure itself is demonstrated with dendrons and external trigger signals. Efﬁcient control over the structure formation is investigated with atomic force microscopy, agarose gel electrophoresis and dynamic light scattering experiments. The external trigger signals can be chosen by choosing the dendron structure. We demonstrate our concept with dendrons which can be triggered either with UV-radiation or with a mild reducing agent such as dithiothretoil. Dielectrophoresis is an electromechanical technique for manipulating micro-and nanoparticles. Here the method is used for developing a new nanoimprint lithographic technique named ﬁeld-induced nanoimprint lithography. In this technique nanoelectrodes are used for producing dielectrophoretically a pattern of nanoparticles on a re-usable master stamp, which is then used for transferring the nanoparticle pattern on a target substrate by mechanical contact. Dielectrophoresis is also used for producing chains of gold nanoparticles between nano- and microelectrodes. The chains are investigated for sensing purposes with impedance studies.

AB - In this work we demonstrate the control of nanoparticles and nanostructures with the help of the DNA origami method and dielectrophoresis. DNA nanotechnology is a subﬁeld of nanotechnology where DNA is used as a construction material. The DNA origami method is a recent development in the ﬁeld which enables the assembly of nanoparticles with nanometer scale accuracy through self-assembly. Here we take advantage of the method for efﬁcient deposition and alignment of single-walled carbon nanotubes (SWCNTs). Especially the alignment of SWCNTs on substrates has been a major challenge for commercialization of SWCNT based devices, to which our approach could offer a potential solution. As an example, a crossed carbon nanotube junction, a basic geometry for a carbon nanotube transistor, is constructed. The high yields of assembled structures as well as correct alignment of SWCNTs are veriﬁed with atomic force microscopy. The DNA origami method is used also for assembling a bow-tie antenna conﬁguration from silver nanoparticles. The optical response of the system is based on the hybridization of the individual nanoparticle surface plasmon resonance modes. The formation of the structures is veriﬁed with transmission electron microscopy and complemented with agarose gel electrophoresis. The conﬁguration could have potential use as an optical DNA sensor. The sensor performance is investigated with ﬁnite-difference time-domain numerical simulations. In addition to assembling nanoparticles, control over the DNA origami structure itself is demonstrated with dendrons and external trigger signals. Efﬁcient control over the structure formation is investigated with atomic force microscopy, agarose gel electrophoresis and dynamic light scattering experiments. The external trigger signals can be chosen by choosing the dendron structure. We demonstrate our concept with dendrons which can be triggered either with UV-radiation or with a mild reducing agent such as dithiothretoil. Dielectrophoresis is an electromechanical technique for manipulating micro-and nanoparticles. Here the method is used for developing a new nanoimprint lithographic technique named ﬁeld-induced nanoimprint lithography. In this technique nanoelectrodes are used for producing dielectrophoretically a pattern of nanoparticles on a re-usable master stamp, which is then used for transferring the nanoparticle pattern on a target substrate by mechanical contact. Dielectrophoresis is also used for producing chains of gold nanoparticles between nano- and microelectrodes. The chains are investigated for sensing purposes with impedance studies.

KW - DNA nanotechnology

KW - DNA origami

KW - electromechanics

KW - nano-optics

KW - DNA-nanoteknologia

KW - DNA origami

KW - elektromekaniikka

KW - nano-optiikka

KW - DNA nanotechnology

KW - DNA origami

KW - electromechanics

KW - nano-optics

M3 - Doctoral Thesis

SN - 978-952-60-5320-2

T3 - Aalto University publication series DOCTORAL DISSERTATIONS

PB - Aalto University

ER -

Nanoscale Assembly Using DNA and Electromagnetic Fields

Abstrakti

Tutkimusalat

Pääsy asiakirjaan

Sormenjälki

Siteeraa tätä