Microfluidic Lateral Flow Cytochrome P450 Assay on a Novel Printed Functionalized Calcium Carbonate-Based Platform for Rapid Screening of Human Xenobiotic Metabolism

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Microfluidic Lateral Flow Cytochrome P450 Assay on a Novel Printed Functionalized Calcium Carbonate-Based Platform for Rapid Screening of Human Xenobiotic Metabolism. / Jutila, Eveliina; Koivunen, Risto; Kiiski, Iiro ; Bollström, Roger; Sikanen, Tiina; Gane, Patrick.

julkaisussa: Advanced Functional Materials, Vuosikerta 28, Nro 31, 1802793, 14.06.2018.

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

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Bibtex - Lataa

@article{5eaa408cdae44b8682fe09784ee13a8f,
title = "Microfluidic Lateral Flow Cytochrome P450 Assay on a Novel Printed Functionalized Calcium Carbonate-Based Platform for Rapid Screening of Human Xenobiotic Metabolism",
abstract = "Cytochrome P450 (CYP) is a superfamily of enzymes in charge of elimination of the majority of clinically used drugs and other xenobiotics. This study focuses on the development of a rapid microfluidic lateral flow assay to study human phase I metabolism reactions mediated by CYP2A6 isoenzyme, the major detoxification route for many known carcinogens and drugs, with coumarin 7-hydroxylation, as the prototype model reaction. Assay fabrication utilizes custom-designed porous functionalized calcium carbonate (FCC) coatings and inkjet-printed fluid barriers. All materials used are novel and carefully chosen to preserve biocompatibility. The design comprises separate zones for reaction, separation and detection, and an absorbent pad to keep the assay wet for extended periods (up to 10 min) even when heated to physiological temperature. The concept enables CYP assays to be made at lower cost than conventional well-plate assays, while providing increased selectivity at equally high speed, owing to the possibility for simultaneous chromatographic separation of the reaction products from the reactants on the FCC coating. The developed concept provides a viable rapid prediction of the interaction risks related to metabolic clearance of drugs and other xenobiotics, and exemplifies a novel coating technology illustrating the opportunity to broaden application functionality.",
keywords = "porous materials, functional coatings, inkjet printing, drug metabolism, cytochrome P450",
author = "Eveliina Jutila and Risto Koivunen and Iiro Kiiski and Roger Bollstr{\"o}m and Tiina Sikanen and Patrick Gane",
year = "2018",
month = "6",
day = "14",
doi = "10.1002/adfm.201802793",
language = "English",
volume = "28",
journal = "Advanced Functional Materials",
issn = "1616-301X",
publisher = "WILEY-VCH VERLAG",
number = "31",

}

RIS - Lataa

TY - JOUR

T1 - Microfluidic Lateral Flow Cytochrome P450 Assay on a Novel Printed Functionalized Calcium Carbonate-Based Platform for Rapid Screening of Human Xenobiotic Metabolism

AU - Jutila, Eveliina

AU - Koivunen, Risto

AU - Kiiski, Iiro

AU - Bollström, Roger

AU - Sikanen, Tiina

AU - Gane, Patrick

PY - 2018/6/14

Y1 - 2018/6/14

N2 - Cytochrome P450 (CYP) is a superfamily of enzymes in charge of elimination of the majority of clinically used drugs and other xenobiotics. This study focuses on the development of a rapid microfluidic lateral flow assay to study human phase I metabolism reactions mediated by CYP2A6 isoenzyme, the major detoxification route for many known carcinogens and drugs, with coumarin 7-hydroxylation, as the prototype model reaction. Assay fabrication utilizes custom-designed porous functionalized calcium carbonate (FCC) coatings and inkjet-printed fluid barriers. All materials used are novel and carefully chosen to preserve biocompatibility. The design comprises separate zones for reaction, separation and detection, and an absorbent pad to keep the assay wet for extended periods (up to 10 min) even when heated to physiological temperature. The concept enables CYP assays to be made at lower cost than conventional well-plate assays, while providing increased selectivity at equally high speed, owing to the possibility for simultaneous chromatographic separation of the reaction products from the reactants on the FCC coating. The developed concept provides a viable rapid prediction of the interaction risks related to metabolic clearance of drugs and other xenobiotics, and exemplifies a novel coating technology illustrating the opportunity to broaden application functionality.

AB - Cytochrome P450 (CYP) is a superfamily of enzymes in charge of elimination of the majority of clinically used drugs and other xenobiotics. This study focuses on the development of a rapid microfluidic lateral flow assay to study human phase I metabolism reactions mediated by CYP2A6 isoenzyme, the major detoxification route for many known carcinogens and drugs, with coumarin 7-hydroxylation, as the prototype model reaction. Assay fabrication utilizes custom-designed porous functionalized calcium carbonate (FCC) coatings and inkjet-printed fluid barriers. All materials used are novel and carefully chosen to preserve biocompatibility. The design comprises separate zones for reaction, separation and detection, and an absorbent pad to keep the assay wet for extended periods (up to 10 min) even when heated to physiological temperature. The concept enables CYP assays to be made at lower cost than conventional well-plate assays, while providing increased selectivity at equally high speed, owing to the possibility for simultaneous chromatographic separation of the reaction products from the reactants on the FCC coating. The developed concept provides a viable rapid prediction of the interaction risks related to metabolic clearance of drugs and other xenobiotics, and exemplifies a novel coating technology illustrating the opportunity to broaden application functionality.

KW - porous materials

KW - functional coatings

KW - inkjet printing

KW - drug metabolism

KW - cytochrome P450

U2 - 10.1002/adfm.201802793

DO - 10.1002/adfm.201802793

M3 - Article

VL - 28

JO - Advanced Functional Materials

JF - Advanced Functional Materials

SN - 1616-301X

IS - 31

M1 - 1802793

ER -

ID: 25728042