Microfluidic Lateral Flow Cytochrome P450 Assay on a Novel Printed Functionalized Calcium Carbonate-Based Platform for Rapid Screening of Human Xenobiotic Metabolism

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Tutkijat

Organisaatiot

  • University of Helsinki
  • Aalto University
  • Omya International AG

Kuvaus

Cytochrome P450 (CYP) is a superfamily of enzymes in charge of
elimination of the majority of clinically used drugs and other xenobiotics.
This study focuses on the development of a rapid microfluidic lateral flow
assay to study human phase I metabolism reactions mediated by CYP2A6
isoenzyme, the major detoxification route for many known carcinogens and
drugs, with coumarin 7-hydroxylation, as the prototype model reaction.
Assay fabrication utilizes custom-designed porous functionalized calcium
carbonate (FCC) coatings and inkjet-printed fluid barriers. All materials
used are novel and carefully chosen to preserve biocompatibility. The
design comprises separate zones for reaction, separation and detection,
and an absorbent pad to keep the assay wet for extended periods
(up to 10 min) even when heated to physiological temperature. The concept
enables CYP assays to be made at lower cost than conventional well-plate
assays, while providing increased selectivity at equally high speed, owing
to the possibility for simultaneous chromatographic separation of the
reaction products from the reactants on the FCC coating. The developed
concept provides a viable rapid prediction of the interaction risks related
to metabolic clearance of drugs and other xenobiotics, and exemplifies a
novel coating technology illustrating the opportunity to broaden application
functionality.

Yksityiskohdat

AlkuperäiskieliEnglanti
Artikkeli1802793
JulkaisuAdvanced Functional Materials
Vuosikerta28
Numero31
TilaJulkaistu - 14 kesäkuuta 2018
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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