Microfluidic devices for biomolecular and cellular analysis

Ari Hokkanen

Microfluidic devices for biomolecular and cellular analysis

Julkaisun otsikon käännös: Mikrofluidistiikkalaitteita biomolekyylien ja solujen analysointiin

Ari Hokkanen

Kemian ja materiaalitieteen laitos

Tutkimustuotos: Doctoral Thesis › Collection of Articles

Abstrakti

Tässä väistöstyössä kehitettiin syöpäkudosleikkeiden analysointiin mikroneulanäytteenottoa, johon suunniteltiin ja valmistettiin uusi kaksikanavainen piimikroneula. Mikroneulanäytteen nestesäiliöksi ja fluidistiikan hallintaan kehitettiin polymeerikanavisto, johon piimikroneula liitettiin liimaamalla. Mikroneulanäytteenoton toimivuus osoitettiin uuttamalla phosphatidylcholine (PC) ja phosphoethanolamine (PE) lipidejä, jotka toimivat biomarkkereina rintasyövälle. Kapillaarielektroforeesiin perustuvaan molekyylien erotuksen kehitettiin pii-lasimikrokanavisto, jolle kasvatettiin amorfisesta piistä elektrodit sähkökentälle. Tätä pii-lasimikrokanavistoa käytettiin testosteronimittauksiin. Kapillaarielektroforeesiin kehitettiin myös kuumapuristettu polymethyl methacrylate (PMMA) kanavisto antibioottiresistanssin mittaamiseen mecA geenin avulla. Tälle PMMA kanavistolle rakennettiin fluoresenssimittaussysteemi, jossa käytettiin laseriin perustuvaa fluoresenssiviritystä ja konfokaalista nelivärimittausta. Lisäksi väitöstyössä kasvatettiin lasialustalle titaanielektrodeja, joita käytettiin sydänkantasolujen sähköiseen stimulaatioon. Sydänkantasolujen lyöntitaajuutta pystyttiin muuttamaan sähköisellä stimulaatiolla 1 sekunnista 0.8 sekuntiin. Mikrofluidistiikka mahdollistaa nopeat terveydenhuollon mittaukset lääkärin vastaanotolla tai jopa kotona ilman aikaa vieviä laboratorioanalyysejä. Hoito ja lääkitys voidaan aloittaa heti ilman parin päivän viivettä välttäen myös turhaa lääkitystä. Mikrofluidistiikkalaitteet toimivat pienillä näytemäärillä ja kuluttavat vähän reagensseja, mikä vähentää kustannuksia verrattuna laboratorioanalyyseihin. Mikrofluidistiikka pystyy tulevaisuudessa tuottamaan tehokkaampaa hoitoa ja isoja säästöjä terveydenhuoltosysteemeihin.

Julkaisun otsikon käännös	Mikrofluidistiikkalaitteita biomolekyylien ja solujen analysointiin
Alkuperäiskieli	Englanti
Pätevyys	Tohtorintutkinto
Myöntävä instituutio	Aalto-yliopisto
Valvoja/neuvonantaja	Franssila, Sami, Vastuuprofessori Franssila, Sami, Ohjaaja
Kustantaja	Aalto University
Painoksen ISBN	978-952-60-6718-6
Sähköinen ISBN	978-952-60-6719-3
Tila	Julkaistu - 2016
OKM-julkaisutyyppi	G5 Artikkeliväitöskirja

Tutkimusalat

mikrofluidistiikka
mikroneula
solu näytealusta
kapillaarielektroforeesi
fluoresenssi

YK:n kestävän kehityksen tavoitteet

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http://urn.fi/URN:ISBN:978-952-60-6719-3Lisenssi: Määrittelemätön

Raaka-aineiden tutkimusinfrastruktuuri
Karppinen, M. (Manager)
Kemian tekniikan korkeakoulu
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@phdthesis{0da0271f499c4c4aa84ed24142d127f3,

title = "Microfluidic devices for biomolecular and cellular analysis",

abstract = "In this thesis microfluidic chips were developed for tissue sampling, chemical separation and electrical cell stimulation. Several fluorescence measurement systems were developed in order to enable small volume measurements in microchannels. Microneedle sampling was developed for cancer tissue analysis. A novel two channel silicon microneedle structure was designed and fabricated. Silicon chip was glue bonded to polymer microfluidic part, which had microfluidic channels as fluid reservoirs and actuation membranes for a fluidic control. Phosphatidylcholine (PC) and phosphoethanolamine (PE) lipids were successfully extracted as biomarkers for breast cancer. Microchip capillary electrophoresis (CE) was shown on silicon-glass chip, which had integrated thin film electrodes for high voltages (HV). This silicon-glass microchip was used for testosterone measurements with LIF detection. Another CE chip was made by hot embossing PMMA (polymethyl methacrylate). It was used to an antibiotic resistance measurement with mecA gene. Confocal four-colour laser induced fluorescence detection system was developed for roll-to-roll hot embossed PMMA chip. Glass chip was developed for human embryonic stem cells stimulation. Titanium thin film electrodes were deposited on glass surface. The native beat cycle of embryonic stem cells could be altered from about 1 s to 800 ms with electrical stimulation. Microfluidics makes fast healthcare analysis possible in doctor's office or even in home without time-consuming laboratory analysis. Treatment and medication could be started immediately without delay also avoiding unnecessary medication. Microfluidic devices require small sample amounts and low reagent consumption, which can lower cost of diagnosis; and tightly localized diagnosis and treatment, even at single cell level, enabling novel treatments. This is expected to bring major savings for healthcare system in future.",

keywords = "microfluidics, microneedle, cell chip, capillary electrophoresis, fluorescence, mikrofluidistiikka, mikroneula, solu n{\"a}ytealusta, kapillaarielektroforeesi, fluoresenssi, microfluidics, microneedle, cell chip, capillary electrophoresis, fluorescence",

author = "Ari Hokkanen",

year = "2016",

language = "English",

isbn = "978-952-60-6718-6",

series = "Aalto University publication series DOCTORAL DISSERTATIONS",

publisher = "Aalto University",

number = "53",

address = "Finland",

school = "Aalto University",

}

TY - BOOK

T1 - Microfluidic devices for biomolecular and cellular analysis

AU - Hokkanen, Ari

PY - 2016

Y1 - 2016

N2 - In this thesis microfluidic chips were developed for tissue sampling, chemical separation and electrical cell stimulation. Several fluorescence measurement systems were developed in order to enable small volume measurements in microchannels. Microneedle sampling was developed for cancer tissue analysis. A novel two channel silicon microneedle structure was designed and fabricated. Silicon chip was glue bonded to polymer microfluidic part, which had microfluidic channels as fluid reservoirs and actuation membranes for a fluidic control. Phosphatidylcholine (PC) and phosphoethanolamine (PE) lipids were successfully extracted as biomarkers for breast cancer. Microchip capillary electrophoresis (CE) was shown on silicon-glass chip, which had integrated thin film electrodes for high voltages (HV). This silicon-glass microchip was used for testosterone measurements with LIF detection. Another CE chip was made by hot embossing PMMA (polymethyl methacrylate). It was used to an antibiotic resistance measurement with mecA gene. Confocal four-colour laser induced fluorescence detection system was developed for roll-to-roll hot embossed PMMA chip. Glass chip was developed for human embryonic stem cells stimulation. Titanium thin film electrodes were deposited on glass surface. The native beat cycle of embryonic stem cells could be altered from about 1 s to 800 ms with electrical stimulation. Microfluidics makes fast healthcare analysis possible in doctor's office or even in home without time-consuming laboratory analysis. Treatment and medication could be started immediately without delay also avoiding unnecessary medication. Microfluidic devices require small sample amounts and low reagent consumption, which can lower cost of diagnosis; and tightly localized diagnosis and treatment, even at single cell level, enabling novel treatments. This is expected to bring major savings for healthcare system in future.

AB - In this thesis microfluidic chips were developed for tissue sampling, chemical separation and electrical cell stimulation. Several fluorescence measurement systems were developed in order to enable small volume measurements in microchannels. Microneedle sampling was developed for cancer tissue analysis. A novel two channel silicon microneedle structure was designed and fabricated. Silicon chip was glue bonded to polymer microfluidic part, which had microfluidic channels as fluid reservoirs and actuation membranes for a fluidic control. Phosphatidylcholine (PC) and phosphoethanolamine (PE) lipids were successfully extracted as biomarkers for breast cancer. Microchip capillary electrophoresis (CE) was shown on silicon-glass chip, which had integrated thin film electrodes for high voltages (HV). This silicon-glass microchip was used for testosterone measurements with LIF detection. Another CE chip was made by hot embossing PMMA (polymethyl methacrylate). It was used to an antibiotic resistance measurement with mecA gene. Confocal four-colour laser induced fluorescence detection system was developed for roll-to-roll hot embossed PMMA chip. Glass chip was developed for human embryonic stem cells stimulation. Titanium thin film electrodes were deposited on glass surface. The native beat cycle of embryonic stem cells could be altered from about 1 s to 800 ms with electrical stimulation. Microfluidics makes fast healthcare analysis possible in doctor's office or even in home without time-consuming laboratory analysis. Treatment and medication could be started immediately without delay also avoiding unnecessary medication. Microfluidic devices require small sample amounts and low reagent consumption, which can lower cost of diagnosis; and tightly localized diagnosis and treatment, even at single cell level, enabling novel treatments. This is expected to bring major savings for healthcare system in future.

KW - microfluidics

KW - microneedle

KW - cell chip

KW - capillary electrophoresis

KW - fluorescence

KW - mikrofluidistiikka

KW - mikroneula

KW - solu näytealusta

KW - kapillaarielektroforeesi

KW - fluoresenssi

KW - microfluidics

KW - microneedle

KW - cell chip

KW - capillary electrophoresis

KW - fluorescence

M3 - Doctoral Thesis

SN - 978-952-60-6718-6

T3 - Aalto University publication series DOCTORAL DISSERTATIONS

PB - Aalto University

ER -

Microfluidic devices for biomolecular and cellular analysis

Abstrakti

Tutkimusalat

YK:n kestävän kehityksen tavoitteet

Pääsy asiakirjaan

Sormenjälki

Laitteet

Raaka-aineiden tutkimusinfrastruktuuri

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