Luminescent markers and labels in authentication and novel bioaffinity assays

Päivi Grönroos

Luminescent markers and labels in authentication and novel bioaffinity assays

Julkaisun otsikon käännös: Luminoivat merkki- ja leima-aineet autentikoinnissa ja bioaffiniteettimäärityksissä

Päivi Grönroos

Tutkimustuotos: Doctoral Thesis › Collection of Articles

Abstrakti

Luminesenssia voidaan hyödyntää useissa kaupallisesti merkittävissä sovelluksissa, kuten väärennösten ehkäisyssä ja bioaffiniteettimäärityksissä. Pienlaitteilla tehtävä vierianalytiikka ja tuotteiden aitouden varmentavat menetelmät ovat tulleet kaiken aikaa yhä merkittävimmiksi, ja kuluttajien itse käyttämät pienlaitteet tulevat olemaan tulevaisuudessa hyvin tärkeässä asemassa. Luminesenssiin perustuva määritysmenetelmä, joka on kehitetty yhteensopivaksi yksinkertaisten laitteistojen, kuten älypuhelinten kanssa, vastaa varsin pitkälti näihin vaatimuksiin. Ympäristöystävällisten valmistusmateriaalien, kuten selluloosan käyttö edistää myös osaltaan sovellusten kiinnostavuutta. Väitöskirjatyössä tutkittiin fibrilliselluloosakalvojen ja kartonkimateriaalien luminesenssiominaisuuksia sekä niiden hyödynnettävyyttä substraatteina luminoiville merkkiaineille. Useiden orgaanisten luminoforien, lantanidikelaattien ja epäorgaanisten fosforien yhdistelmiä hyödynnettiin aitouden varmentamiseen käytettävissä tietokannoissa luminesenssitopografiakarttojen muodossa. Koko aitouden tarkistamisprosessi, merkinnän valovirityksestä tuotteen tunnistamiseen, toteutettiin myös älypuhelinsovelluksella. Laajaa valikoimaa erilaisia merkkiaineita voidaan hyödyntää tulevaisuudessa älypuhelinten kameratekniikan ja laskentatehon kehittyessä. Selluloosalla havaittiin esiintyvän useasta luminesenssikeskuksesta johtuvaa pitkäikäistä luminesenssia, jota voidaan myös tarvittaessa hyödyntää aitousmerkinnän pakkausmateriaalikohtaisena sisäisenä standardina. Väitöskirjatyössä tutkittiin myös selluloosapohjaisista komposiittimateriaaleista valmistettuja elektrodeja edullisina vaihtoehtoina oksidipäällystetyille piielektrodeille elektrokemiluminoimmunomäärityksissä. Selluloosajohdannaisia ja polystyreeniä käytettiin eristemateriaalina, ja hiilimustaa ja grafiittia johdemateriaalina komposiittimusteissa, joista valmistettiin silkkipainettuja elektrodisiruja. Elektrodien analyyttinen sovellettavuus testattiin C-reaktiivisen proteiinin määrityksellä käytettäessä leima-aineina Tb(III)kelaattia ja fluoreseiini-isotiosyanaattia (FITC). Selluloosapohjaiset komposiittimateriaalit soveltuvat hyvin silkkipainettujen elektrodien valmistukseen, ja niillä todettiin olevan matala taustaemissio, laaja lineaarinen alue ja matalat toteamisrajat. FITC:llä voidaan saada jopa matalampia toteamisrajoja kuin Tb(III)kelaatilla, mikä on lupaava ominaisuus kustannuksiltaan edullisia vierianalytiikan sovelluksia ajatellen.

Julkaisun otsikon käännös	Luminoivat merkki- ja leima-aineet autentikoinnissa ja bioaffiniteettimäärityksissä
Alkuperäiskieli	Englanti
Pätevyys	Tohtorintutkinto
Myöntävä instituutio	Aalto-yliopisto
Valvoja/neuvonantaja	Kulmala, Sakari, Vastuuprofessori Kulmala, Sakari, Ohjaaja
Kustantaja	Aalto University
Painoksen ISBN	978-952-60-3820-9
Sähköinen ISBN	978-952-60-3821-6
Tila	Julkaistu - 2020
OKM-julkaisutyyppi	G5 Artikkeliväitöskirja

Tutkimusalat

luminoforit
autentikointi
selluloosa
komposiittielektrodit
bioaffiniteettimääritykset

Pääsy asiakirjaan

http://urn.fi/URN:ISBN:978-952-60-3821-6

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@phdthesis{69045e5cc6bd406199e9a595a5e815b9,

title = "Luminescent markers and labels in authentication and novel bioaffinity assays",

abstract = "Luminescence can be utilized in various commercially important applications such as anti-counterfeit (AC) markings and bioaffinity assays. The increasing demands for product authentication by consumers, and point-of-care (POC) health monitoring systems, can be met using luminescence based solutions developed to be compatible with simple instrumentation such as smartphones. Use of environmentally friendly materials like cellulose would further benefit such applications. In this thesis, the luminescence properties of fibrillary cellulose films and cardboard materials are studied to find a cellulose based material usable as a substrate for luminescent AC markers. Multiple combinations of organic luminophores, lanthanide chelates and inorganic phosphors have been used to create datasets i.e. luminescence topography maps (LTM) for verification of the authenticity of the product package. The whole authentication process, from the photoexcitation of the marker to the recognition of the product, is demonstrated with a smartphone application. Wide variety of marker luminophores can be used in the future, as camera technologies and computing power of the smartphones are constantly evolving. The results also show that cellulose as substrate displays weak long-lived photoluminescence emission. This emission originating from multiple luminescence centers, can be utilized in LTM as an internal standard in the method. Cellulose based composite materials are also investigated as low-cost alternatives for expensive oxide-coated silicon electrodes for electrochemiluminoimmunoassays. Composite electrodes made of insulating cellulose derivatives or polystyrene with different conductive carbon materials are studied, and these materials are used to make screen-printed electrode chips. The analytical applicability of the electrodes have been tested in immunoassay of C-reactive protein with Tb(III)chelate and fluorescein isothiocyanate (FITC) labels. The results indicate that conductive carbon-cellulose composite materials are well suited for the fabrication of screen-printed electrodes. Low background emission, wide linear range and low detection limits are obtained with these electrodes. FITC could provide lower detection limits of the analyte than the presently used Tb(III) chelate labels, which is very promising for low-cost POCT applications.",

keywords = "luminophores, authentication, cellulose, composite electrodes, bioaffinity assays, luminoforit, autentikointi, selluloosa, komposiittielektrodit, bioaffiniteettim{\"a}{\"a}ritykset, luminophores, authentication, cellulose, composite electrodes, bioaffinity assays",

author = "P{\"a}ivi Gr{\"o}nroos",

year = "2020",

language = "English",

isbn = "978-952-60-3820-9",

series = "Aalto University publication series DOCTORAL DISSERTATIONS",

publisher = "Aalto University",

number = "53",

address = "Finland",

school = "Aalto University",

}

TY - BOOK

T1 - Luminescent markers and labels in authentication and novel bioaffinity assays

AU - Grönroos, Päivi

PY - 2020

Y1 - 2020

N2 - Luminescence can be utilized in various commercially important applications such as anti-counterfeit (AC) markings and bioaffinity assays. The increasing demands for product authentication by consumers, and point-of-care (POC) health monitoring systems, can be met using luminescence based solutions developed to be compatible with simple instrumentation such as smartphones. Use of environmentally friendly materials like cellulose would further benefit such applications. In this thesis, the luminescence properties of fibrillary cellulose films and cardboard materials are studied to find a cellulose based material usable as a substrate for luminescent AC markers. Multiple combinations of organic luminophores, lanthanide chelates and inorganic phosphors have been used to create datasets i.e. luminescence topography maps (LTM) for verification of the authenticity of the product package. The whole authentication process, from the photoexcitation of the marker to the recognition of the product, is demonstrated with a smartphone application. Wide variety of marker luminophores can be used in the future, as camera technologies and computing power of the smartphones are constantly evolving. The results also show that cellulose as substrate displays weak long-lived photoluminescence emission. This emission originating from multiple luminescence centers, can be utilized in LTM as an internal standard in the method. Cellulose based composite materials are also investigated as low-cost alternatives for expensive oxide-coated silicon electrodes for electrochemiluminoimmunoassays. Composite electrodes made of insulating cellulose derivatives or polystyrene with different conductive carbon materials are studied, and these materials are used to make screen-printed electrode chips. The analytical applicability of the electrodes have been tested in immunoassay of C-reactive protein with Tb(III)chelate and fluorescein isothiocyanate (FITC) labels. The results indicate that conductive carbon-cellulose composite materials are well suited for the fabrication of screen-printed electrodes. Low background emission, wide linear range and low detection limits are obtained with these electrodes. FITC could provide lower detection limits of the analyte than the presently used Tb(III) chelate labels, which is very promising for low-cost POCT applications.

AB - Luminescence can be utilized in various commercially important applications such as anti-counterfeit (AC) markings and bioaffinity assays. The increasing demands for product authentication by consumers, and point-of-care (POC) health monitoring systems, can be met using luminescence based solutions developed to be compatible with simple instrumentation such as smartphones. Use of environmentally friendly materials like cellulose would further benefit such applications. In this thesis, the luminescence properties of fibrillary cellulose films and cardboard materials are studied to find a cellulose based material usable as a substrate for luminescent AC markers. Multiple combinations of organic luminophores, lanthanide chelates and inorganic phosphors have been used to create datasets i.e. luminescence topography maps (LTM) for verification of the authenticity of the product package. The whole authentication process, from the photoexcitation of the marker to the recognition of the product, is demonstrated with a smartphone application. Wide variety of marker luminophores can be used in the future, as camera technologies and computing power of the smartphones are constantly evolving. The results also show that cellulose as substrate displays weak long-lived photoluminescence emission. This emission originating from multiple luminescence centers, can be utilized in LTM as an internal standard in the method. Cellulose based composite materials are also investigated as low-cost alternatives for expensive oxide-coated silicon electrodes for electrochemiluminoimmunoassays. Composite electrodes made of insulating cellulose derivatives or polystyrene with different conductive carbon materials are studied, and these materials are used to make screen-printed electrode chips. The analytical applicability of the electrodes have been tested in immunoassay of C-reactive protein with Tb(III)chelate and fluorescein isothiocyanate (FITC) labels. The results indicate that conductive carbon-cellulose composite materials are well suited for the fabrication of screen-printed electrodes. Low background emission, wide linear range and low detection limits are obtained with these electrodes. FITC could provide lower detection limits of the analyte than the presently used Tb(III) chelate labels, which is very promising for low-cost POCT applications.

KW - luminophores

KW - authentication

KW - cellulose

KW - composite electrodes

KW - bioaffinity assays

KW - luminoforit

KW - autentikointi

KW - selluloosa

KW - komposiittielektrodit

KW - bioaffiniteettimääritykset

KW - luminophores

KW - authentication

KW - cellulose

KW - composite electrodes

KW - bioaffinity assays

M3 - Doctoral Thesis

SN - 978-952-60-3820-9

T3 - Aalto University publication series DOCTORAL DISSERTATIONS

PB - Aalto University

ER -

Luminescent markers and labels in authentication and novel bioaffinity assays

Abstrakti

Tutkimusalat

Pääsy asiakirjaan

Sormenjälki

Laitteet

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