Landscape of RNA polyadenylation in E. coli

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Standard

Landscape of RNA polyadenylation in E. coli. / Maes, Alexandre; Gracia, Céline; Innocenti, Nicolas; Zhang, Kaiyang; Aurell, Erik; Hajnsdorf, Eliane.

julkaisussa: Nucleic Acids Research, Vuosikerta 45, Nro 5, 17.03.2017, s. 2746-2756.

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Harvard

Maes, A, Gracia, C, Innocenti, N, Zhang, K, Aurell, E & Hajnsdorf, E 2017, 'Landscape of RNA polyadenylation in E. coli' Nucleic Acids Research, Vuosikerta. 45, Nro 5, Sivut 2746-2756. https://doi.org/10.1093/nar/gkw894

APA

Maes, A., Gracia, C., Innocenti, N., Zhang, K., Aurell, E., & Hajnsdorf, E. (2017). Landscape of RNA polyadenylation in E. coli. Nucleic Acids Research, 45(5), 2746-2756. https://doi.org/10.1093/nar/gkw894

Vancouver

Maes A, Gracia C, Innocenti N, Zhang K, Aurell E, Hajnsdorf E. Landscape of RNA polyadenylation in E. coli. Nucleic Acids Research. 2017 maalis 17;45(5):2746-2756. https://doi.org/10.1093/nar/gkw894

Author

Maes, Alexandre ; Gracia, Céline ; Innocenti, Nicolas ; Zhang, Kaiyang ; Aurell, Erik ; Hajnsdorf, Eliane. / Landscape of RNA polyadenylation in E. coli. Julkaisussa: Nucleic Acids Research. 2017 ; Vuosikerta 45, Nro 5. Sivut 2746-2756.

Bibtex - Lataa

@article{88b86c2e05bf4c2298fc80db9377328f,
title = "Landscape of RNA polyadenylation in E. coli",
abstract = "Polyadenylation is thought to be involved in the degradation and quality control of bacterial RNAs but relatively few examples have been investigated. We used a combination of 5'-tagRACE and RNA-seq to analyze the total RNA content from a wild-type strain and froma poly(A)polymerase deletedmutant. A total of 178 transcripts were either up- or down-regulated in the mutant when compared to the wild-type strain. Poly(A)polymerase up-regulates the expression of all genes related to the FliA regulon and several previously unknown transcripts, including numerous transporters. Notable down-regulation of genes in the expression of antigen 43 and components of the type 1 fimbriae was detected. The major consequence of the absence of poly(A)polymerase was the accumulation of numerous sRNAs, antisense transcripts, REP sequences and RNA fragments resulting from the processing of entire transcripts. A new algorithm to analyze the position and composition of post-transcriptional modifications based on the sequence of unencoded 3'-ends, was developed to identify polyadenylated molecules. Overall our results shed new light on the broad spectrum of action of polyadenylation on gene expression and demonstrate the importance of poly(A) dependent degradation to remove structured RNA fragments.",
author = "Alexandre Maes and C{\'e}line Gracia and Nicolas Innocenti and Kaiyang Zhang and Erik Aurell and Eliane Hajnsdorf",
year = "2017",
month = "3",
day = "17",
doi = "10.1093/nar/gkw894",
language = "English",
volume = "45",
pages = "2746--2756",
journal = "Nucleic Acids Research",
issn = "0305-1048",
number = "5",

}

RIS - Lataa

TY - JOUR

T1 - Landscape of RNA polyadenylation in E. coli

AU - Maes, Alexandre

AU - Gracia, Céline

AU - Innocenti, Nicolas

AU - Zhang, Kaiyang

AU - Aurell, Erik

AU - Hajnsdorf, Eliane

PY - 2017/3/17

Y1 - 2017/3/17

N2 - Polyadenylation is thought to be involved in the degradation and quality control of bacterial RNAs but relatively few examples have been investigated. We used a combination of 5'-tagRACE and RNA-seq to analyze the total RNA content from a wild-type strain and froma poly(A)polymerase deletedmutant. A total of 178 transcripts were either up- or down-regulated in the mutant when compared to the wild-type strain. Poly(A)polymerase up-regulates the expression of all genes related to the FliA regulon and several previously unknown transcripts, including numerous transporters. Notable down-regulation of genes in the expression of antigen 43 and components of the type 1 fimbriae was detected. The major consequence of the absence of poly(A)polymerase was the accumulation of numerous sRNAs, antisense transcripts, REP sequences and RNA fragments resulting from the processing of entire transcripts. A new algorithm to analyze the position and composition of post-transcriptional modifications based on the sequence of unencoded 3'-ends, was developed to identify polyadenylated molecules. Overall our results shed new light on the broad spectrum of action of polyadenylation on gene expression and demonstrate the importance of poly(A) dependent degradation to remove structured RNA fragments.

AB - Polyadenylation is thought to be involved in the degradation and quality control of bacterial RNAs but relatively few examples have been investigated. We used a combination of 5'-tagRACE and RNA-seq to analyze the total RNA content from a wild-type strain and froma poly(A)polymerase deletedmutant. A total of 178 transcripts were either up- or down-regulated in the mutant when compared to the wild-type strain. Poly(A)polymerase up-regulates the expression of all genes related to the FliA regulon and several previously unknown transcripts, including numerous transporters. Notable down-regulation of genes in the expression of antigen 43 and components of the type 1 fimbriae was detected. The major consequence of the absence of poly(A)polymerase was the accumulation of numerous sRNAs, antisense transcripts, REP sequences and RNA fragments resulting from the processing of entire transcripts. A new algorithm to analyze the position and composition of post-transcriptional modifications based on the sequence of unencoded 3'-ends, was developed to identify polyadenylated molecules. Overall our results shed new light on the broad spectrum of action of polyadenylation on gene expression and demonstrate the importance of poly(A) dependent degradation to remove structured RNA fragments.

UR - http://www.scopus.com/inward/record.url?scp=85018357344&partnerID=8YFLogxK

U2 - 10.1093/nar/gkw894

DO - 10.1093/nar/gkw894

M3 - Article

VL - 45

SP - 2746

EP - 2756

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 5

ER -

ID: 13273925