Interactions between model cell membranes and the neuroactive drug propofol

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

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Interactions between model cell membranes and the neuroactive drug propofol. / Niga, Petru; Hansson-Mille, Petra M.; Swerin, Agne; Claesson, Per M.; Schoelkopf, Joachim; Gane, Patrick A.C.; Bergendal, Erik; Tummino, Andrea; Campbell, Richard A.; Magnus Johnson, C.

julkaisussa: Journal of Colloid and Interface Science, Vuosikerta 526, 15.09.2018, s. 230-243.

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Harvard

Niga, P, Hansson-Mille, PM, Swerin, A, Claesson, PM, Schoelkopf, J, Gane, PAC, Bergendal, E, Tummino, A, Campbell, RA & Magnus Johnson, C 2018, 'Interactions between model cell membranes and the neuroactive drug propofol', Journal of Colloid and Interface Science, Vuosikerta. 526, Sivut 230-243. https://doi.org/10.1016/j.jcis.2018.03.052

APA

Niga, P., Hansson-Mille, P. M., Swerin, A., Claesson, P. M., Schoelkopf, J., Gane, P. A. C., ... Magnus Johnson, C. (2018). Interactions between model cell membranes and the neuroactive drug propofol. Journal of Colloid and Interface Science, 526, 230-243. https://doi.org/10.1016/j.jcis.2018.03.052

Vancouver

Author

Niga, Petru ; Hansson-Mille, Petra M. ; Swerin, Agne ; Claesson, Per M. ; Schoelkopf, Joachim ; Gane, Patrick A.C. ; Bergendal, Erik ; Tummino, Andrea ; Campbell, Richard A. ; Magnus Johnson, C. / Interactions between model cell membranes and the neuroactive drug propofol. Julkaisussa: Journal of Colloid and Interface Science. 2018 ; Vuosikerta 526. Sivut 230-243.

Bibtex - Lataa

@article{696e6c052c7e43a3ab7f3bd627679789,
title = "Interactions between model cell membranes and the neuroactive drug propofol",
abstract = "Vibrational sum frequency spectroscopy (VSFS) complemented by surface pressure isotherm and neutron reflectometry (NR) experiments were employed to investigate the interactions between propofol, a small amphiphilic molecule that currently is the most common general anaesthetic drug, and phospholipid monolayers. A series of biologically relevant saturated phospholipids of varying chain length from C18 to C14 were spread on either pure water or propofol (2,6-bis(1-methylethyl)phenol) solution in a Langmuir trough, and the change in the molecular structure of the film, induced by the interaction with propofol, was studied with respect to the surface pressure. The results from the surface pressure isotherm experiments revealed that propofol, as long as it remains at the interface, enhances the fluidity of the phospholipid monolayer. The VSF spectra demonstrate that for each phospholipid the amount of propofol in the monolayer region decreases with increasing surface pressure. Such squeeze out is in contrast to the enhanced interactions that can be exhibited by more complex amphiphilic molecules such as peptides. At surface pressures of 22–25 mN m−1, which are relevant for biological cell membranes, most of the propofol has been expelled from the monolayer, especially in the case of the C16 and C18 phospholipids that adopt a liquid condensed phase packing of its alkyl tails. At lower surface pressures of 5 mN m−1, the effect of propofol on the structure of the alkyl tails is enhanced when the phospholipids are present in a liquid expanded phase. Specifically, for the C16 phospholipid, NR data reveal that propofol is located exclusively in the head group region, which is rationalized in the context of previous studies. The results imply a non-homogeneous distribution of propofol in the plane of real cell membranes, which is an inference that requires urgent testing and may help to explain why such low concentration of the drug are required to induce general anaesthesia.",
keywords = "Langmuir trough, Model membrane, Neutron reflectometry, Phospholipid monolayers, Propofol, Small amphiphilic drug, Surface pressure isotherm, Vibrational sum frequency spectroscopy",
author = "Petru Niga and Hansson-Mille, {Petra M.} and Agne Swerin and Claesson, {Per M.} and Joachim Schoelkopf and Gane, {Patrick A.C.} and Erik Bergendal and Andrea Tummino and Campbell, {Richard A.} and {Magnus Johnson}, C.",
year = "2018",
month = "9",
day = "15",
doi = "10.1016/j.jcis.2018.03.052",
language = "English",
volume = "526",
pages = "230--243",
journal = "Journal of Colloid and Interface Science",
issn = "0021-9797",

}

RIS - Lataa

TY - JOUR

T1 - Interactions between model cell membranes and the neuroactive drug propofol

AU - Niga, Petru

AU - Hansson-Mille, Petra M.

AU - Swerin, Agne

AU - Claesson, Per M.

AU - Schoelkopf, Joachim

AU - Gane, Patrick A.C.

AU - Bergendal, Erik

AU - Tummino, Andrea

AU - Campbell, Richard A.

AU - Magnus Johnson, C.

PY - 2018/9/15

Y1 - 2018/9/15

N2 - Vibrational sum frequency spectroscopy (VSFS) complemented by surface pressure isotherm and neutron reflectometry (NR) experiments were employed to investigate the interactions between propofol, a small amphiphilic molecule that currently is the most common general anaesthetic drug, and phospholipid monolayers. A series of biologically relevant saturated phospholipids of varying chain length from C18 to C14 were spread on either pure water or propofol (2,6-bis(1-methylethyl)phenol) solution in a Langmuir trough, and the change in the molecular structure of the film, induced by the interaction with propofol, was studied with respect to the surface pressure. The results from the surface pressure isotherm experiments revealed that propofol, as long as it remains at the interface, enhances the fluidity of the phospholipid monolayer. The VSF spectra demonstrate that for each phospholipid the amount of propofol in the monolayer region decreases with increasing surface pressure. Such squeeze out is in contrast to the enhanced interactions that can be exhibited by more complex amphiphilic molecules such as peptides. At surface pressures of 22–25 mN m−1, which are relevant for biological cell membranes, most of the propofol has been expelled from the monolayer, especially in the case of the C16 and C18 phospholipids that adopt a liquid condensed phase packing of its alkyl tails. At lower surface pressures of 5 mN m−1, the effect of propofol on the structure of the alkyl tails is enhanced when the phospholipids are present in a liquid expanded phase. Specifically, for the C16 phospholipid, NR data reveal that propofol is located exclusively in the head group region, which is rationalized in the context of previous studies. The results imply a non-homogeneous distribution of propofol in the plane of real cell membranes, which is an inference that requires urgent testing and may help to explain why such low concentration of the drug are required to induce general anaesthesia.

AB - Vibrational sum frequency spectroscopy (VSFS) complemented by surface pressure isotherm and neutron reflectometry (NR) experiments were employed to investigate the interactions between propofol, a small amphiphilic molecule that currently is the most common general anaesthetic drug, and phospholipid monolayers. A series of biologically relevant saturated phospholipids of varying chain length from C18 to C14 were spread on either pure water or propofol (2,6-bis(1-methylethyl)phenol) solution in a Langmuir trough, and the change in the molecular structure of the film, induced by the interaction with propofol, was studied with respect to the surface pressure. The results from the surface pressure isotherm experiments revealed that propofol, as long as it remains at the interface, enhances the fluidity of the phospholipid monolayer. The VSF spectra demonstrate that for each phospholipid the amount of propofol in the monolayer region decreases with increasing surface pressure. Such squeeze out is in contrast to the enhanced interactions that can be exhibited by more complex amphiphilic molecules such as peptides. At surface pressures of 22–25 mN m−1, which are relevant for biological cell membranes, most of the propofol has been expelled from the monolayer, especially in the case of the C16 and C18 phospholipids that adopt a liquid condensed phase packing of its alkyl tails. At lower surface pressures of 5 mN m−1, the effect of propofol on the structure of the alkyl tails is enhanced when the phospholipids are present in a liquid expanded phase. Specifically, for the C16 phospholipid, NR data reveal that propofol is located exclusively in the head group region, which is rationalized in the context of previous studies. The results imply a non-homogeneous distribution of propofol in the plane of real cell membranes, which is an inference that requires urgent testing and may help to explain why such low concentration of the drug are required to induce general anaesthesia.

KW - Langmuir trough

KW - Model membrane

KW - Neutron reflectometry

KW - Phospholipid monolayers

KW - Propofol

KW - Small amphiphilic drug

KW - Surface pressure isotherm

KW - Vibrational sum frequency spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=85046641029&partnerID=8YFLogxK

U2 - 10.1016/j.jcis.2018.03.052

DO - 10.1016/j.jcis.2018.03.052

M3 - Article

VL - 526

SP - 230

EP - 243

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

SN - 0021-9797

ER -

ID: 21592796