In vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activity

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Standard

In vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activity. / Yuryev, Mikhail; Andriichuk, Liliia; Leiwe, Marcus; Jokinen, Ville; Carabalona, Aurelie; Rivera, Claudio.

julkaisussa: Scientific Reports, Vuosikerta 8, Nro 1, 30.10.2018.

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Harvard

APA

Vancouver

Author

Yuryev, Mikhail ; Andriichuk, Liliia ; Leiwe, Marcus ; Jokinen, Ville ; Carabalona, Aurelie ; Rivera, Claudio. / In vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activity. Julkaisussa: Scientific Reports. 2018 ; Vuosikerta 8, Nro 1.

Bibtex - Lataa

@article{68cf1eb35aa04192a9c7e5fae88540c5,
title = "In vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activity",
abstract = "Prior to sensory experience spontaneous activity appears to play a fundamental role in the correct formation of prominent functional features of different cortical regions. The use of anaesthesia during pregnancy such as ketamine is largely considered to negatively affect neuronal development by interfering with synaptic transmission. Interestingly, the characteristics of spontaneous activity as well as the acute functional effects of maternal anaesthesia remain largely untested in the embryonic cortex in vivo. In the present work, we performed in vivo imaging of spontaneous calcium activity and cell motility in the marginal zone of the cortex of E14-15 embryos connected to the mother. We made use of a preparation where the blood circulation from the mother through the umbilical cord is preserved and fluctuations in intracellular calcium in the embryonic frontal cortex are acquired using two-photon imaging. We found that spontaneous transients were either sporadic or correlated in clusters of neuronal ensembles at this age. These events were not sensitive to maternal isoflurane anaesthesia but were strongly inhibited by acute in situ or maternal application of low concentration of the anaesthetic ketamine (a non-competitive antagonist of NMDA receptors). Moreover, simultaneous imaging of cell motility revealed a correlated strong sensitivity to ketamine. These results show that anaesthetic compounds can differ significantly in their impact on spontaneous early cortical activity as well as motility of cells in the marginal zone. The effects found in this study may be relevant in the etiology of heightened vulnerability to cerebral dysfunction associated with the use of ketamine during pregnancy.",
author = "Mikhail Yuryev and Liliia Andriichuk and Marcus Leiwe and Ville Jokinen and Aurelie Carabalona and Claudio Rivera",
year = "2018",
month = "10",
day = "30",
doi = "10.1038/s41598-018-34410-x",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
number = "1",

}

RIS - Lataa

TY - JOUR

T1 - In vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activity

AU - Yuryev, Mikhail

AU - Andriichuk, Liliia

AU - Leiwe, Marcus

AU - Jokinen, Ville

AU - Carabalona, Aurelie

AU - Rivera, Claudio

PY - 2018/10/30

Y1 - 2018/10/30

N2 - Prior to sensory experience spontaneous activity appears to play a fundamental role in the correct formation of prominent functional features of different cortical regions. The use of anaesthesia during pregnancy such as ketamine is largely considered to negatively affect neuronal development by interfering with synaptic transmission. Interestingly, the characteristics of spontaneous activity as well as the acute functional effects of maternal anaesthesia remain largely untested in the embryonic cortex in vivo. In the present work, we performed in vivo imaging of spontaneous calcium activity and cell motility in the marginal zone of the cortex of E14-15 embryos connected to the mother. We made use of a preparation where the blood circulation from the mother through the umbilical cord is preserved and fluctuations in intracellular calcium in the embryonic frontal cortex are acquired using two-photon imaging. We found that spontaneous transients were either sporadic or correlated in clusters of neuronal ensembles at this age. These events were not sensitive to maternal isoflurane anaesthesia but were strongly inhibited by acute in situ or maternal application of low concentration of the anaesthetic ketamine (a non-competitive antagonist of NMDA receptors). Moreover, simultaneous imaging of cell motility revealed a correlated strong sensitivity to ketamine. These results show that anaesthetic compounds can differ significantly in their impact on spontaneous early cortical activity as well as motility of cells in the marginal zone. The effects found in this study may be relevant in the etiology of heightened vulnerability to cerebral dysfunction associated with the use of ketamine during pregnancy.

AB - Prior to sensory experience spontaneous activity appears to play a fundamental role in the correct formation of prominent functional features of different cortical regions. The use of anaesthesia during pregnancy such as ketamine is largely considered to negatively affect neuronal development by interfering with synaptic transmission. Interestingly, the characteristics of spontaneous activity as well as the acute functional effects of maternal anaesthesia remain largely untested in the embryonic cortex in vivo. In the present work, we performed in vivo imaging of spontaneous calcium activity and cell motility in the marginal zone of the cortex of E14-15 embryos connected to the mother. We made use of a preparation where the blood circulation from the mother through the umbilical cord is preserved and fluctuations in intracellular calcium in the embryonic frontal cortex are acquired using two-photon imaging. We found that spontaneous transients were either sporadic or correlated in clusters of neuronal ensembles at this age. These events were not sensitive to maternal isoflurane anaesthesia but were strongly inhibited by acute in situ or maternal application of low concentration of the anaesthetic ketamine (a non-competitive antagonist of NMDA receptors). Moreover, simultaneous imaging of cell motility revealed a correlated strong sensitivity to ketamine. These results show that anaesthetic compounds can differ significantly in their impact on spontaneous early cortical activity as well as motility of cells in the marginal zone. The effects found in this study may be relevant in the etiology of heightened vulnerability to cerebral dysfunction associated with the use of ketamine during pregnancy.

UR - http://www.scopus.com/inward/record.url?scp=85055618563&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-34410-x

DO - 10.1038/s41598-018-34410-x

M3 - Article

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

ER -

ID: 29460636