Immobilization of membrane-bound enzymes on micropillar arrays via fusogenic liposomes

This work reports a new immobilization strategy for membrane-bound enzymes, such as the cytochromes P450 (CYPs), the major class of drug metabolizing enzymes. Our immobilization method relies on natural biotinylation of the lipid membrane with the help of fusogenic liposomes. With our method, CYPs (both human and recombinant) can be immobilized on streptavidin-functionalized micropillar arrays without affecting the enzyme kinetic parameters or stability, which has been challenging to most of the previously reported approaches, such as physical entrapment or covalent bonding of the enzymes.