Hydrophilic modification of polystyrene with hydrophobin for time-resolved immunofluorometric assay

Zefang Wang, Yujian Huang, Shan Li, Haijin Xu*, Markus B. Linder, Mingqiang Qiao

*Tämän työn vastaava kirjoittaja

Tutkimustuotos: LehtiartikkeliArticleScientificvertaisarvioitu

38 Sitaatiot (Scopus)

Abstrakti

Herein we reported that a hydrophobin film was used as a solid support on the polystyrene surface for immobilizing antibodies in the time-resolved immunofluorometric assay (TR-IFMA). Quartz crystal microbalance with dissipative monitoring (QCM-D), X-ray photoelectron spectroscopy (XPS) and water contact angle (WCA) measurements, as well as atomic force microscope (AFM) were used to characterize the hydrophilic modification of polystyrene surface with Class I hydrophobin isolated from Grifola frondosa (HGFI). The performance of HGFI-modified polystyrene was evaluated by TR-IFMA of carcinoembryonic antigen (CEA). QCM-D revealed that HGFI formed an intact monolayer on the polystyrene at pH 5. XPS and WCA measurements showed that self-assembling HGFI could render polystyrene surface hydrophilic for three months. AFM indicated that an end-on antibody monolayer was adsorbed on the HGFI film rather than multilayers on the polystyrene in a side-on orientation. Furthermore, a linear calibration curve (from 5 to 600. ng/mL) of CEA showed HGFI-modified polystyrene had higher detection sensitivity than unmodified ones in TR-IFMA. This present method for modifying polystyrene is simple without severe chemical treatment and may have wide applicability to functionalize other supports for immobilizing biomolecules.

AlkuperäiskieliEnglanti
Sivut1074-1079
Sivumäärä6
JulkaisuBiosensors and Bioelectronics
Vuosikerta26
Numero3
DOI - pysyväislinkit
TilaJulkaistu - 15 marraskuuta 2010
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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