High-affinity and selective detection of pyrophosphate in water by a resorcinarene salt receptor

Ngong Kodiah Beyeh; Isabel Díez; S. Maryamdokht Taimoory; Daniel Meister; Andrew I. Feig; John F. Trant; Robin H.A. Ras; Kari Rissanen

doi:10.1039/c7sc05167k

High-affinity and selective detection of pyrophosphate in water by a resorcinarene salt receptor

Ngong Kodiah Beyeh^*, Isabel Díez, S. Maryamdokht Taimoory, Daniel Meister, Andrew I. Feig, John F. Trant, Robin H.A. Ras, Kari Rissanen

^*Tämän työn vastaava kirjoittaja

Tutkimustuotos: Lehtiartikkeli › Article › Scientific › vertaisarvioitu

47 Sitaatiot (Scopus)

145 Lataukset (Pure)

Abstrakti

Pyrophosphate (PPi) is a byproduct of DNA and RNA synthesis, and abnormal levels are indicative of disease. We report the high-affinity binding of PPi in water by N-alkyl ammonium resorcinarene chloride receptors. Experimental analysis using ¹H and ³¹P NMR, isothermal titration calorimetry, mass spectrometry, and UV-vis spectroscopy all support exceptional selectivity of these systems for PPi in water. The measured affinity of K₁ = 1.60 × 10⁷ M^-1 for PPi is three orders of magnitude larger than that observed for binding to another phosphate, ATP. This exceptional anion-binding affinity in water is explored through a detailed density functional theory computational study. These systems provide a promising avenue for the development of future innovative medical diagnostic tools.

Alkuperäiskieli	Englanti
Sivut	1358-1367
Sivumäärä	10
Julkaisu	Chemical Science
Vuosikerta	9
Numero	5
DOI - pysyväislinkit	https://doi.org/10.1039/c7sc05167k
Tila	Julkaistu - 2018
OKM-julkaisutyyppi	A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

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10.1039/c7sc05167kLisenssi: CC BY

c7sc05167kFinal published version, 1,69 MBLisenssi: CC BY

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title = "High-affinity and selective detection of pyrophosphate in water by a resorcinarene salt receptor",

abstract = "Pyrophosphate (PPi) is a byproduct of DNA and RNA synthesis, and abnormal levels are indicative of disease. We report the high-affinity binding of PPi in water by N-alkyl ammonium resorcinarene chloride receptors. Experimental analysis using 1H and 31P NMR, isothermal titration calorimetry, mass spectrometry, and UV-vis spectroscopy all support exceptional selectivity of these systems for PPi in water. The measured affinity of K1 = 1.60 × 107 M-1 for PPi is three orders of magnitude larger than that observed for binding to another phosphate, ATP. This exceptional anion-binding affinity in water is explored through a detailed density functional theory computational study. These systems provide a promising avenue for the development of future innovative medical diagnostic tools.",

author = "Beyeh, {Ngong Kodiah} and Isabel D{\'i}ez and Taimoory, {S. Maryamdokht} and Daniel Meister and Feig, {Andrew I.} and Trant, {John F.} and Ras, {Robin H.A.} and Kari Rissanen",

year = "2018",

doi = "10.1039/c7sc05167k",

language = "English",

volume = "9",

pages = "1358--1367",

journal = "Chemical Science",

issn = "2041-6520",

publisher = "Royal Society of Chemistry",

number = "5",

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TY - JOUR

T1 - High-affinity and selective detection of pyrophosphate in water by a resorcinarene salt receptor

AU - Beyeh, Ngong Kodiah

AU - Díez, Isabel

AU - Taimoory, S. Maryamdokht

AU - Meister, Daniel

AU - Feig, Andrew I.

AU - Trant, John F.

AU - Ras, Robin H.A.

AU - Rissanen, Kari

PY - 2018

Y1 - 2018

N2 - Pyrophosphate (PPi) is a byproduct of DNA and RNA synthesis, and abnormal levels are indicative of disease. We report the high-affinity binding of PPi in water by N-alkyl ammonium resorcinarene chloride receptors. Experimental analysis using 1H and 31P NMR, isothermal titration calorimetry, mass spectrometry, and UV-vis spectroscopy all support exceptional selectivity of these systems for PPi in water. The measured affinity of K1 = 1.60 × 107 M-1 for PPi is three orders of magnitude larger than that observed for binding to another phosphate, ATP. This exceptional anion-binding affinity in water is explored through a detailed density functional theory computational study. These systems provide a promising avenue for the development of future innovative medical diagnostic tools.

AB - Pyrophosphate (PPi) is a byproduct of DNA and RNA synthesis, and abnormal levels are indicative of disease. We report the high-affinity binding of PPi in water by N-alkyl ammonium resorcinarene chloride receptors. Experimental analysis using 1H and 31P NMR, isothermal titration calorimetry, mass spectrometry, and UV-vis spectroscopy all support exceptional selectivity of these systems for PPi in water. The measured affinity of K1 = 1.60 × 107 M-1 for PPi is three orders of magnitude larger than that observed for binding to another phosphate, ATP. This exceptional anion-binding affinity in water is explored through a detailed density functional theory computational study. These systems provide a promising avenue for the development of future innovative medical diagnostic tools.

UR - http://www.scopus.com/inward/record.url?scp=85041443358&partnerID=8YFLogxK

U2 - 10.1039/c7sc05167k

DO - 10.1039/c7sc05167k

M3 - Article

AN - SCOPUS:85041443358

SN - 2041-6520

VL - 9

SP - 1358

EP - 1367

JO - Chemical Science

JF - Chemical Science

IS - 5

ER -

High-affinity and selective detection of pyrophosphate in water by a resorcinarene salt receptor

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