Functional Liquid-Fluid Interfaces Based onHydrophobin Proteins: - An Experimental Study for Medical Applications

Hedar H. Al-Terke

Functional Liquid-Fluid Interfaces Based onHydrophobin Proteins: - An Experimental Study for Medical Applications

Julkaisun otsikon käännös: Hydrofobiini Proteiineihin perustuvat funktionaaliset neste-fluidi-rajapinnat: Kokeellinen tutkimus lääketieteellisiin sovelluksiin

Hedar H. Al-Terke

Tutkimustuotos: Doctoral Thesis › Collection of Articles

Abstrakti

Rajapintoja on kaikkialla ympärillämme. Kaikki suorat vuorovaikutukset tapahtuvat rajapinnalla. Väitöskirjassa tutkittiin toiminnallisten rajapintojen sovelluksia erilaisten lääketieteellisten haasteiden ratkaisemiseen. Väitöskirjan neljä julkaisua tuovat esiin eri tapoja, joilla toiminnallisia rajapintoja voidaan hyödyntää näiden haasteiden ratkaisemisessa. Julkaisu I keskittyi painovoiman, viskoelastisuuden ja HFBI-hydrofobiini proteiineilla päällystettyjen vesipisaroiden muodon väliseen suhteeseen. Tutkimalla hydrofobiinien itsejärjestymistä ilman ja veden rajapinnalla havaittiin, että rajapinnalle muodostuu jäykkä kerros kriittisessä pitoisuudessa, mikä vaikut¬taa pisaran muotoon. Tällä havainnolla on merkittäviä vaikutuksia teknisiin ja biolääke-tieteellisiin sovelluksiin, koska se tarjoaa reitin pisaroiden muodon hallintaan eri järjestelmissä. Julkaisussa II esiteltiin uusi vasta-aineiden eristysmenetelmä, jossa käytetään kehittyneitä materi-aaleja ja toiminnallisia rajapintoja. Jakamalla öljypohjainen ferrofluidi pieniksi pisaroiksi ulkoisen magneettikentän alla kasvattaen neste-neste rajapintojen pinta-alaa, mikä mahdollistaa näiden rajapintojen toiminnallistamisen ja soveltamisen substraattina vasta-aineiden eristämistä varten. Julkaisu III keskittyi proteiinipäällysteisten kaasukuplien muodostumiseen ja karakterisointiin. Arvioimme mikropipetti-imutekniikalla kuplien mekaaniset ominaisuudet ja määrittelimme tiivistysparametrin (Q) kuplien kaasunläpäisevyyden arvioimiseksi. Näillä hyvin karakterisoiduilla kuplilla on lupaava potentiaali ultraäänikontrastiaineina eri biolääketieteen aloilla. Niitä voidaan hyödyntää kuvantamistarkoituksiin ja lääkkeiden kohdennettuun annosteluun, mikä avaa uusia mahdollisuuksia lääketieteelliseen diagnostiikkaan ja hoitoihin. Julkaisussa IV tutkittiin hydrofobiiniproteiinilla toiminnallistettujen kuplien hyödyntämistä kehittyneen ultraäänimolekyylikuvantamiskoettimen kehittämisessä. Toiminnallistetut kuplat, joiden rajapintaan on kiinnitetty sopiva antigeeni, voivat paljastaa sairaita soluja, kuten syöpäsoluja. Tämä innovatiivinen lähestymistapa tarjoaa suuria lupauksia molekyylikuvantamistekniikoiden tarkkuuden ja herkkyyden parantamisessa, mikä mahdollistaa sairauksien varhaisen havaitsemisen ja tarkan kohdentamisen. Kaiken kaikkiaan tässä väitöskirjassa esitetyt havainnot osoittavat toiminnallisten rajapintojen valtavan potentiaalin erilaisten lääketieteellisten sovellusten haasteiden ratkaisemisessa. Ne tarjoavat arvokasta tietoa uusien materiaalien ja tekniikoiden suunnittelusta ja kehittämisestä, jotka voivat parantaa diagnostiikkaa, hoitoja ja kuvantamista biolääketieteen alalla.

Julkaisun otsikon käännös	Hydrofobiini Proteiineihin perustuvat funktionaaliset neste-fluidi-rajapinnat: Kokeellinen tutkimus lääketieteellisiin sovelluksiin
Alkuperäiskieli	Englanti
Pätevyys	Tohtorintutkinto
Myöntävä instituutio	Aalto-yliopisto
Valvoja/neuvonantaja	Ras, Robin, Vastuuprofessori Paananen, Arja, Ohjaaja, Ulkoinen henkilö Joensuu, Jussi, Ohjaaja, Ulkoinen henkilö
Kustantaja	Aalto University
Painoksen ISBN	978-952-64-1814-8
Sähköinen ISBN	978-952-64-1815-5
Tila	Julkaistu - 2024
OKM-julkaisutyyppi	G5 Artikkeliväitöskirja

Tutkimusalat

toiminnalliset rajapinnat
proteiinit
kuplat
pisarat
molekyylikuvaus

YK:n kestävän kehityksen tavoitteet

Tämä tuotos edistää seuraavia kestävän kehityksen tavoitteita:

Pääsy asiakirjaan

http://urn.fi/URN:ISBN:978-952-64-1825-4

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Rissanen, A. (Manager)
Aalto-yliopisto
Laitteistot/tilat: Facility
OtaNano Nanomikroskopiakeskus
Seitsonen, J. (Manager) & Rissanen, A. (Other)
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@phdthesis{c9e673b0c1ca46cd99c5565bf97247cf,

title = "Functional Liquid-Fluid Interfaces Based onHydrophobin Proteins: - An Experimental Study for Medical Applications",

abstract = "Interfaces are everywhere around us. Any direct interaction occurs at the interface. This thesis explored the potential of functional interfaces to solve various medical application challenges. The four publications presented in this thesis highlight the different ways in which functional interfaces can be utilized to address these challenges. Publication I focused on the relationship between gravity, viscoelasticity, and the shape of water droplets coated with HFBI hydrophobin proteins. By studying the self-organization of hydrophobins at the air-water interface, it was found that a rigid layer is formed at a critical concentration, which affects the droplet morphology. This finding has significant implications for engineering and biomedical applications, as it provides a pathway for controlling the shape of droplets in various systems. Publication II presented a novel antibody extraction method using advanced materials and functional interfaces. By dividing an oil-based ferrofluid into daughter droplets under an external magnetic field, the surface area of liquid-liquid interfaces is increased, allowing for the functionalization and application of these interfaces as a substrate for antibody extraction. Publication III focused on the formation and characterization of protein-coated gas bubbles. By employing a micropipette aspiration technique, the mechanical properties of these bubbles were assessed, and a sealing parameter (Q) was determined to evaluate their gas permeability. These well-characterized bubbles have promising potential as ultrasound-enhanced contrast agents in various biomedical fields. They can be utilized for imaging purposes and targeted drug delivery, opening up new possibilities for medical diagnostics and therapies. Publication IV explored the utilization of hydrophobin protein functionalized bubbles to develop an advanced ultrasound molecular imaging probe. By functionalizing bubbles with a moiety part at their interface, they can attach to specific antigens and reveal diseased cells, such as cancer cells. This innovative approach holds great promise for improving the accuracy and sensitivity of molecular imaging techniques, enabling early detection and precise targeting of diseases. Overall, the findings presented in this thesis demonstrate the immense potential of functional interfaces in solving various medical application challenges. They provide valuable insights into the design and development of novel materials and techniques that can improve diagnostics, therapeutics, and imaging in the biomedical field.",

keywords = "functional interfaces, proteins, bubbles, droplets, molecular imaging, toiminnalliset rajapinnat, proteiinit, kuplat, pisarat, molekyylikuvaus, functional interfaces, proteins, bubbles, droplets, molecular imaging",

author = "Al-Terke, {Hedar H.}",

year = "2024",

language = "English",

isbn = "978-952-64-1814-8",

series = "Aalto University publication series DOCTORAL THESES",

publisher = "Aalto University",

number = "99",

address = "Finland",

school = "Aalto University",

}

TY - BOOK

T1 - Functional Liquid-Fluid Interfaces Based onHydrophobin Proteins: - An Experimental Study for Medical Applications

AU - Al-Terke, Hedar H.

PY - 2024

Y1 - 2024

N2 - Interfaces are everywhere around us. Any direct interaction occurs at the interface. This thesis explored the potential of functional interfaces to solve various medical application challenges. The four publications presented in this thesis highlight the different ways in which functional interfaces can be utilized to address these challenges. Publication I focused on the relationship between gravity, viscoelasticity, and the shape of water droplets coated with HFBI hydrophobin proteins. By studying the self-organization of hydrophobins at the air-water interface, it was found that a rigid layer is formed at a critical concentration, which affects the droplet morphology. This finding has significant implications for engineering and biomedical applications, as it provides a pathway for controlling the shape of droplets in various systems. Publication II presented a novel antibody extraction method using advanced materials and functional interfaces. By dividing an oil-based ferrofluid into daughter droplets under an external magnetic field, the surface area of liquid-liquid interfaces is increased, allowing for the functionalization and application of these interfaces as a substrate for antibody extraction. Publication III focused on the formation and characterization of protein-coated gas bubbles. By employing a micropipette aspiration technique, the mechanical properties of these bubbles were assessed, and a sealing parameter (Q) was determined to evaluate their gas permeability. These well-characterized bubbles have promising potential as ultrasound-enhanced contrast agents in various biomedical fields. They can be utilized for imaging purposes and targeted drug delivery, opening up new possibilities for medical diagnostics and therapies. Publication IV explored the utilization of hydrophobin protein functionalized bubbles to develop an advanced ultrasound molecular imaging probe. By functionalizing bubbles with a moiety part at their interface, they can attach to specific antigens and reveal diseased cells, such as cancer cells. This innovative approach holds great promise for improving the accuracy and sensitivity of molecular imaging techniques, enabling early detection and precise targeting of diseases. Overall, the findings presented in this thesis demonstrate the immense potential of functional interfaces in solving various medical application challenges. They provide valuable insights into the design and development of novel materials and techniques that can improve diagnostics, therapeutics, and imaging in the biomedical field.

AB - Interfaces are everywhere around us. Any direct interaction occurs at the interface. This thesis explored the potential of functional interfaces to solve various medical application challenges. The four publications presented in this thesis highlight the different ways in which functional interfaces can be utilized to address these challenges. Publication I focused on the relationship between gravity, viscoelasticity, and the shape of water droplets coated with HFBI hydrophobin proteins. By studying the self-organization of hydrophobins at the air-water interface, it was found that a rigid layer is formed at a critical concentration, which affects the droplet morphology. This finding has significant implications for engineering and biomedical applications, as it provides a pathway for controlling the shape of droplets in various systems. Publication II presented a novel antibody extraction method using advanced materials and functional interfaces. By dividing an oil-based ferrofluid into daughter droplets under an external magnetic field, the surface area of liquid-liquid interfaces is increased, allowing for the functionalization and application of these interfaces as a substrate for antibody extraction. Publication III focused on the formation and characterization of protein-coated gas bubbles. By employing a micropipette aspiration technique, the mechanical properties of these bubbles were assessed, and a sealing parameter (Q) was determined to evaluate their gas permeability. These well-characterized bubbles have promising potential as ultrasound-enhanced contrast agents in various biomedical fields. They can be utilized for imaging purposes and targeted drug delivery, opening up new possibilities for medical diagnostics and therapies. Publication IV explored the utilization of hydrophobin protein functionalized bubbles to develop an advanced ultrasound molecular imaging probe. By functionalizing bubbles with a moiety part at their interface, they can attach to specific antigens and reveal diseased cells, such as cancer cells. This innovative approach holds great promise for improving the accuracy and sensitivity of molecular imaging techniques, enabling early detection and precise targeting of diseases. Overall, the findings presented in this thesis demonstrate the immense potential of functional interfaces in solving various medical application challenges. They provide valuable insights into the design and development of novel materials and techniques that can improve diagnostics, therapeutics, and imaging in the biomedical field.

KW - functional interfaces

KW - proteins

KW - bubbles

KW - droplets

KW - molecular imaging

KW - toiminnalliset rajapinnat

KW - proteiinit

KW - kuplat

KW - pisarat

KW - molekyylikuvaus

KW - functional interfaces

KW - proteins

KW - bubbles

KW - droplets

KW - molecular imaging

M3 - Doctoral Thesis

SN - 978-952-64-1814-8

T3 - Aalto University publication series DOCTORAL THESES

PB - Aalto University

ER -

Functional Liquid-Fluid Interfaces Based onHydrophobin Proteins: - An Experimental Study for Medical Applications

Abstrakti

Tutkimusalat

YK:n kestävän kehityksen tavoitteet

Pääsy asiakirjaan

Sormenjälki

Laitteet

OtaNano

OtaNano Nanomikroskopiakeskus

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