Exploring the mineralization of hydrophobins at a liquid interface

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Tutkijat

  • Alexander Schulz
  • Marco Fioroni
  • Markus Linder

  • Andreas Nessel
  • Marco Bocola
  • Thomas Subkowski
  • Ulrich Schwaneberg
  • Alexander Böker
  • Francisco Rodríguez-Ropero

Organisaatiot

  • VTT Technical Research Centre of Finland
  • RWTH Aachen University
  • BASF
  • Technische Universität Darmstadt

Kuvaus

Hydrophobins, a class of highly surface active amphiphilic proteins, can stabilize various interfaces. When used for emulsion stabilization, it has been recently shown that they are able to induce mineralization, resulting in hollow capsules. We found that not all types of hydrophobins trigger mineralization, and that the morphology of the mineral changes depending on the selected oil. We investigated the formation of hydrophobin films at interfaces by the use of CD spectroscopy. In order to elucidate the structural features that enhance the mineralization property and give a possible explanation for this behavior, we performed MD-simulations of two representative hydrophobins (EAS for class I and HFBII for class II) at a hexane-water interface, in the presence as well as in the absence of ions. Our studies showed that the class II hydrophobin HFBII, which did not induce mineralization, only slightly changes its structure during adsorption at the oil-water interface or upon addition of Ca2+ and HPO4 2- ions. In contrast to that, the class I hydrophobin EAS changed its conformation to a large extent during the adsorption and interacted strongly with added ions. We revealed that EAS preorganizes the ions at short distances matching the lattice dimensions of hydroxyapatite. The latter finding yields a straightforward explanation for the observed differences in mineralization behavior and allows us to search for other hydrophobins that could assist mineralization, despite their different functions in nature.

Yksityiskohdat

AlkuperäiskieliEnglanti
Sivut11343-11352
Sivumäärä10
JulkaisuSoft Matter
Vuosikerta8
Numero44
TilaJulkaistu - 2012
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

ID: 4808719