Effect of Glycosylation on Self-Assembly of Lipid A Lipopolysaccharides in Aqueous Solutions

Valeria Castelletto; Jani Seitsonen; Ian W. Hamley

doi:10.1021/acs.langmuir.3c00828

Effect of Glycosylation on Self-Assembly of Lipid A Lipopolysaccharides in Aqueous Solutions

Valeria Castelletto^*
, Jani Seitsonen
, Ian W. Hamley

^*Tämän työn vastaava kirjoittaja

OtaNano

University of Reading

Tutkimustuotos: Lehtiartikkeli › Article › Scientific › vertaisarvioitu

6 Sitaatiot (Scopus)

67 Lataukset (Pure)

Abstrakti

Lipopolysaccharides (LPSs) based on lipid A produced by bacteria are of interest due to their bioactivity in stimulating immune responses, as are simpler synthetic components or analogues. Here, the self-assembly in water of two monodisperse lipid A derivatives based on simplified bacterial LPS structures is examined and compared to that of a native Escherichia coli LPS using small-angle X-ray scattering and cryogenic transmission electron microscopy. The critical aggregation concentration is obtained from fluorescence probe experiments, and conformation is probed using circular dichroism spectroscopy. The E. coli LPS is found to form wormlike micelles, whereas the synthetic analogues bearing six lipid chains and with four or two saccharide head groups (Kdo₂-lipid A and monophosphoryl lipid A) self-assemble into nanosheets or vesicles, respectively. These observations are rationalized by considering the surfactant packing parameter.

Alkuperäiskieli	Englanti
Sivut	8516-8522
Sivumäärä	7
Julkaisu	Langmuir
Vuosikerta	39
Numero	24
DOI - pysyväislinkit	https://doi.org/10.1021/acs.langmuir.3c00828
Tila	Julkaistu - 20 kesäk. 2023
OKM-julkaisutyyppi	A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Rahoitus

This work was supported by an EPSRC Fellowship grant (reference EP/V053396/1) to I.W.H. We thank Diamond for the award of SAXS beamtime on B21 (ref. SM29895-1) and Nikul Khunti for assistance and the ESRF for beamtime on BM29 (ref. MX-2513) and Dihia Moussaoui for help. We acknowledge the use of facilities in the Chemical Analysis Facility (CAF) at the University of Reading.

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10.1021/acs.langmuir.3c00828Lisenssi: CC BY

SCI_Castelletto_etal_Langmuir_2023Final published version, 3,62 MBLisenssi: CC BY

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@article{2401e2bd4990463ca6ed2f6bd2ae5d9d,

title = "Effect of Glycosylation on Self-Assembly of Lipid A Lipopolysaccharides in Aqueous Solutions",

abstract = "Lipopolysaccharides (LPSs) based on lipid A produced by bacteria are of interest due to their bioactivity in stimulating immune responses, as are simpler synthetic components or analogues. Here, the self-assembly in water of two monodisperse lipid A derivatives based on simplified bacterial LPS structures is examined and compared to that of a native Escherichia coli LPS using small-angle X-ray scattering and cryogenic transmission electron microscopy. The critical aggregation concentration is obtained from fluorescence probe experiments, and conformation is probed using circular dichroism spectroscopy. The E. coli LPS is found to form wormlike micelles, whereas the synthetic analogues bearing six lipid chains and with four or two saccharide head groups (Kdo2-lipid A and monophosphoryl lipid A) self-assemble into nanosheets or vesicles, respectively. These observations are rationalized by considering the surfactant packing parameter.",

author = "Valeria Castelletto and Jani Seitsonen and Hamley, \{Ian W.\}",

note = "Funding Information: This work was supported by an EPSRC Fellowship grant (reference EP/V053396/1) to I.W.H. We thank Diamond for the award of SAXS beamtime on B21 (ref. SM29895-1) and Nikul Khunti for assistance and the ESRF for beamtime on BM29 (ref. MX-2513) and Dihia Moussaoui for help. We acknowledge the use of facilities in the Chemical Analysis Facility (CAF) at the University of Reading. Publisher Copyright: {\textcopyright} 2023 The Authors. Published by American Chemical Society.",

year = "2023",

month = jun,

day = "20",

doi = "10.1021/acs.langmuir.3c00828",

language = "English",

volume = "39",

pages = "8516--8522",

journal = "Langmuir",

issn = "0743-7463",

publisher = "American Chemical Society",

number = "24",

}

TY - JOUR

T1 - Effect of Glycosylation on Self-Assembly of Lipid A Lipopolysaccharides in Aqueous Solutions

AU - Castelletto, Valeria

AU - Seitsonen, Jani

AU - Hamley, Ian W.

N1 - Funding Information: This work was supported by an EPSRC Fellowship grant (reference EP/V053396/1) to I.W.H. We thank Diamond for the award of SAXS beamtime on B21 (ref. SM29895-1) and Nikul Khunti for assistance and the ESRF for beamtime on BM29 (ref. MX-2513) and Dihia Moussaoui for help. We acknowledge the use of facilities in the Chemical Analysis Facility (CAF) at the University of Reading. Publisher Copyright: © 2023 The Authors. Published by American Chemical Society.

PY - 2023/6/20

Y1 - 2023/6/20

N2 - Lipopolysaccharides (LPSs) based on lipid A produced by bacteria are of interest due to their bioactivity in stimulating immune responses, as are simpler synthetic components or analogues. Here, the self-assembly in water of two monodisperse lipid A derivatives based on simplified bacterial LPS structures is examined and compared to that of a native Escherichia coli LPS using small-angle X-ray scattering and cryogenic transmission electron microscopy. The critical aggregation concentration is obtained from fluorescence probe experiments, and conformation is probed using circular dichroism spectroscopy. The E. coli LPS is found to form wormlike micelles, whereas the synthetic analogues bearing six lipid chains and with four or two saccharide head groups (Kdo2-lipid A and monophosphoryl lipid A) self-assemble into nanosheets or vesicles, respectively. These observations are rationalized by considering the surfactant packing parameter.

AB - Lipopolysaccharides (LPSs) based on lipid A produced by bacteria are of interest due to their bioactivity in stimulating immune responses, as are simpler synthetic components or analogues. Here, the self-assembly in water of two monodisperse lipid A derivatives based on simplified bacterial LPS structures is examined and compared to that of a native Escherichia coli LPS using small-angle X-ray scattering and cryogenic transmission electron microscopy. The critical aggregation concentration is obtained from fluorescence probe experiments, and conformation is probed using circular dichroism spectroscopy. The E. coli LPS is found to form wormlike micelles, whereas the synthetic analogues bearing six lipid chains and with four or two saccharide head groups (Kdo2-lipid A and monophosphoryl lipid A) self-assemble into nanosheets or vesicles, respectively. These observations are rationalized by considering the surfactant packing parameter.

UR - https://www.scopus.com/pages/publications/85163455209

U2 - 10.1021/acs.langmuir.3c00828

DO - 10.1021/acs.langmuir.3c00828

M3 - Article

C2 - 37289534

AN - SCOPUS:85163455209

SN - 0743-7463

VL - 39

SP - 8516

EP - 8522

JO - Langmuir

JF - Langmuir

IS - 24

ER -

Effect of Glycosylation on Self-Assembly of Lipid A Lipopolysaccharides in Aqueous Solutions

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