This paper deals with the study of the influence of the lay-up configuration on interlaminar and in-plane shear properties of glass fiber reinforced epoxy composites. The following laminates were produced by resin transfer molding with vacuum assistance for this study: 5, 5, [0/90/0/90/0] and randomly oriented (mat). The composites, with similar overall fiber volume fraction, were evaluated based on four tests: double-notched shear, short beam shear, V-notched rail and Iosipescu shear tests. Besides, the dynamic shear modulus was measured with non-destructive testing based on free vibration method. The 5 laminate presented interlaminar shear strength almost twice that of 5, whereas the mat samples presented higher in-plane shear strength in both tests used due to its random fiber orientation. The dynamic shear modulus was higher for the composites 5, as expected due to the longitudinally oriented fibers. Among the shear test methods applied, double-notched and V-notched methods exhibited more auspicious features, possibly due to a more uniform shear stress state during testing.