Effect of cellulase family and structure on modification of wood fibres at high consistency

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Effect of cellulase family and structure on modification of wood fibres at high consistency. / Rahikainen, Jenni; Ceccherini, Sara; Molinier, Matthieu; Holopainen-Mantila, Ulla; Reza, Mehedi; Väisänen, Saija; Puranen, Terhi; Kruus, Kristiina; Vuorinen, Tapani; Maloney, Thaddeus; Suurnäkki, Anna; Grönqvist, Stina.

julkaisussa: Cellulose, Vuosikerta 26, Nro 8, 05.2019, s. 5085-5103.

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

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Rahikainen, Jenni ; Ceccherini, Sara ; Molinier, Matthieu ; Holopainen-Mantila, Ulla ; Reza, Mehedi ; Väisänen, Saija ; Puranen, Terhi ; Kruus, Kristiina ; Vuorinen, Tapani ; Maloney, Thaddeus ; Suurnäkki, Anna ; Grönqvist, Stina. / Effect of cellulase family and structure on modification of wood fibres at high consistency. Julkaisussa: Cellulose. 2019 ; Vuosikerta 26, Nro 8. Sivut 5085-5103.

Bibtex - Lataa

@article{6e7daef33ab0436d8a64bb30a77ec3f1,
title = "Effect of cellulase family and structure on modification of wood fibres at high consistency",
abstract = "Abstract: Enzymatic modification of bleached softwood kraft fibres for improved fibre reactivity was studied at high (20{\%} w/w) and low (1{\%} w/w) dry matter content. The role of enzyme family and structure in fibre modification was assessed using endoglucanases from three structurally different glycoside hydrolase (GH) families (5, 7 and 45) with and without a carbohydrate binding module (CBM). Based on the amount of dissolved sugars, enzyme action at high consistency was about sixfold higher compared to a fibre treatment at low consistency. The GH45 endoglucanase was found to be most specific in acting on pulp cellulose whereas the family 5 and 7 endoglucanases had activity also on pulp hemicelluloses. The GH45 endoglucanase was found to be most efficient in reducing molecular weight and viscosity of the pulp. In addition, treatment with the GH45 endoglucanase resulted in the highest micropore volume in fibres and thus an increase in cellulose accessibility. The increased accessibility could be seen as decreased dissolution time in cupriethylenediamine using recently developed analytical techniques: viscometric analysis and microscopic video analysis. At high consistency, CBM was not promoting enzyme action, although CBMs are known to be beneficial at low dry matter conditions. Graphical abstract: [Figure not available: see fulltext.].",
keywords = "Dissolution, Endoglucanase, Enzyme, Fibre, Fibre reactivity, High consistency",
author = "Jenni Rahikainen and Sara Ceccherini and Matthieu Molinier and Ulla Holopainen-Mantila and Mehedi Reza and Saija V{\"a}is{\"a}nen and Terhi Puranen and Kristiina Kruus and Tapani Vuorinen and Thaddeus Maloney and Anna Suurn{\"a}kki and Stina Gr{\"o}nqvist",
year = "2019",
month = "5",
doi = "10.1007/s10570-019-02424-x",
language = "English",
volume = "26",
pages = "5085--5103",
journal = "Cellulose",
issn = "0969-0239",
number = "8",

}

RIS - Lataa

TY - JOUR

T1 - Effect of cellulase family and structure on modification of wood fibres at high consistency

AU - Rahikainen, Jenni

AU - Ceccherini, Sara

AU - Molinier, Matthieu

AU - Holopainen-Mantila, Ulla

AU - Reza, Mehedi

AU - Väisänen, Saija

AU - Puranen, Terhi

AU - Kruus, Kristiina

AU - Vuorinen, Tapani

AU - Maloney, Thaddeus

AU - Suurnäkki, Anna

AU - Grönqvist, Stina

PY - 2019/5

Y1 - 2019/5

N2 - Abstract: Enzymatic modification of bleached softwood kraft fibres for improved fibre reactivity was studied at high (20% w/w) and low (1% w/w) dry matter content. The role of enzyme family and structure in fibre modification was assessed using endoglucanases from three structurally different glycoside hydrolase (GH) families (5, 7 and 45) with and without a carbohydrate binding module (CBM). Based on the amount of dissolved sugars, enzyme action at high consistency was about sixfold higher compared to a fibre treatment at low consistency. The GH45 endoglucanase was found to be most specific in acting on pulp cellulose whereas the family 5 and 7 endoglucanases had activity also on pulp hemicelluloses. The GH45 endoglucanase was found to be most efficient in reducing molecular weight and viscosity of the pulp. In addition, treatment with the GH45 endoglucanase resulted in the highest micropore volume in fibres and thus an increase in cellulose accessibility. The increased accessibility could be seen as decreased dissolution time in cupriethylenediamine using recently developed analytical techniques: viscometric analysis and microscopic video analysis. At high consistency, CBM was not promoting enzyme action, although CBMs are known to be beneficial at low dry matter conditions. Graphical abstract: [Figure not available: see fulltext.].

AB - Abstract: Enzymatic modification of bleached softwood kraft fibres for improved fibre reactivity was studied at high (20% w/w) and low (1% w/w) dry matter content. The role of enzyme family and structure in fibre modification was assessed using endoglucanases from three structurally different glycoside hydrolase (GH) families (5, 7 and 45) with and without a carbohydrate binding module (CBM). Based on the amount of dissolved sugars, enzyme action at high consistency was about sixfold higher compared to a fibre treatment at low consistency. The GH45 endoglucanase was found to be most specific in acting on pulp cellulose whereas the family 5 and 7 endoglucanases had activity also on pulp hemicelluloses. The GH45 endoglucanase was found to be most efficient in reducing molecular weight and viscosity of the pulp. In addition, treatment with the GH45 endoglucanase resulted in the highest micropore volume in fibres and thus an increase in cellulose accessibility. The increased accessibility could be seen as decreased dissolution time in cupriethylenediamine using recently developed analytical techniques: viscometric analysis and microscopic video analysis. At high consistency, CBM was not promoting enzyme action, although CBMs are known to be beneficial at low dry matter conditions. Graphical abstract: [Figure not available: see fulltext.].

KW - Dissolution

KW - Endoglucanase

KW - Enzyme

KW - Fibre

KW - Fibre reactivity

KW - High consistency

UR - http://www.scopus.com/inward/record.url?scp=85064495223&partnerID=8YFLogxK

U2 - 10.1007/s10570-019-02424-x

DO - 10.1007/s10570-019-02424-x

M3 - Article

VL - 26

SP - 5085

EP - 5103

JO - Cellulose

JF - Cellulose

SN - 0969-0239

IS - 8

ER -

ID: 33572896