Dissecting the effect of chemical additives on the enzymatic hydrolysis of pretreated wheat straw

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Dissecting the effect of chemical additives on the enzymatic hydrolysis of pretreated wheat straw. / Monschein, Mareike; Reisinger, Christoph; Nidetzky, Bernd.

julkaisussa: Bioresource Technology, Vuosikerta 169, 2014, s. 713-722.

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

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Monschein, Mareike ; Reisinger, Christoph ; Nidetzky, Bernd. / Dissecting the effect of chemical additives on the enzymatic hydrolysis of pretreated wheat straw. Julkaisussa: Bioresource Technology. 2014 ; Vuosikerta 169. Sivut 713-722.

Bibtex - Lataa

@article{fb3694a2284f48e8add51bdbee96d215,
title = "Dissecting the effect of chemical additives on the enzymatic hydrolysis of pretreated wheat straw",
abstract = "Chemical additives were examined for ability to increase the enzymatic hydrolysis of thermo-acidically pretreated wheat straw by Trichoderma reesei cellulase at 50°C. Semi-empirical descriptors derived from the hydrolysis time courses were applied to compare influence of these additives on lignocellulose bioconversion on a kinetic level, presenting a novel view on their mechanism of action. Focus was on rate retardation during hydrolysis, substrate conversion and enzyme adsorption. PEG 8000 enabled a reduction of enzyme loading by 50{\%} while retaining the same conversion of 67{\%} after 24h. For the first time, a beneficial effect of urea is reported, increasing the final substrate conversion after 48h by 16{\%}. The cationic surfactant cetyl-trimethylammonium bromide (CTAB) enhanced the hydrolysis rate at extended reaction time (rlim) by 34{\%} and reduced reaction time by 28{\%}. A combination of PEG 8000 and urea increased sugar release more than additives used individually.",
keywords = "Cellulase, Chemical additives, Enzymatic hydrolysis, Protein adsorption, Urea",
author = "Mareike Monschein and Christoph Reisinger and Bernd Nidetzky",
year = "2014",
doi = "10.1016/j.biortech.2014.07.054",
language = "English",
volume = "169",
pages = "713--722",
journal = "Bioresource Technology",
issn = "0960-8524",

}

RIS - Lataa

TY - JOUR

T1 - Dissecting the effect of chemical additives on the enzymatic hydrolysis of pretreated wheat straw

AU - Monschein, Mareike

AU - Reisinger, Christoph

AU - Nidetzky, Bernd

PY - 2014

Y1 - 2014

N2 - Chemical additives were examined for ability to increase the enzymatic hydrolysis of thermo-acidically pretreated wheat straw by Trichoderma reesei cellulase at 50°C. Semi-empirical descriptors derived from the hydrolysis time courses were applied to compare influence of these additives on lignocellulose bioconversion on a kinetic level, presenting a novel view on their mechanism of action. Focus was on rate retardation during hydrolysis, substrate conversion and enzyme adsorption. PEG 8000 enabled a reduction of enzyme loading by 50% while retaining the same conversion of 67% after 24h. For the first time, a beneficial effect of urea is reported, increasing the final substrate conversion after 48h by 16%. The cationic surfactant cetyl-trimethylammonium bromide (CTAB) enhanced the hydrolysis rate at extended reaction time (rlim) by 34% and reduced reaction time by 28%. A combination of PEG 8000 and urea increased sugar release more than additives used individually.

AB - Chemical additives were examined for ability to increase the enzymatic hydrolysis of thermo-acidically pretreated wheat straw by Trichoderma reesei cellulase at 50°C. Semi-empirical descriptors derived from the hydrolysis time courses were applied to compare influence of these additives on lignocellulose bioconversion on a kinetic level, presenting a novel view on their mechanism of action. Focus was on rate retardation during hydrolysis, substrate conversion and enzyme adsorption. PEG 8000 enabled a reduction of enzyme loading by 50% while retaining the same conversion of 67% after 24h. For the first time, a beneficial effect of urea is reported, increasing the final substrate conversion after 48h by 16%. The cationic surfactant cetyl-trimethylammonium bromide (CTAB) enhanced the hydrolysis rate at extended reaction time (rlim) by 34% and reduced reaction time by 28%. A combination of PEG 8000 and urea increased sugar release more than additives used individually.

KW - Cellulase

KW - Chemical additives

KW - Enzymatic hydrolysis

KW - Protein adsorption

KW - Urea

UR - http://www.scopus.com/inward/record.url?scp=84907340177&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2014.07.054

DO - 10.1016/j.biortech.2014.07.054

M3 - Article

VL - 169

SP - 713

EP - 722

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -

ID: 13017918