TY - JOUR
T1 - Discovery of novel secreted fungal sulfhydryl oxidases with a plate test screen
AU - Nivala, Outi
AU - Mattinen, Maija Liisa
AU - Faccio, Greta
AU - Buchert, Johanna
AU - Kruus, Kristiina
PY - 2013/11/1
Y1 - 2013/11/1
N2 - Sulfhydryl oxidases (SOX) are FAD-dependent enzymes capable of oxidising free thiol groups and forming disulphide bonds. Although the quantity of scientific papers and suggested applications for SOX is constantly increasing, only a limited number of microbial SOX have been reported and are commercially available. Hence, the aim of this study was to develop a fast and reliable qualitative plate test for screening novel secreted fungal SOX. The screening was based on the Ellman's reagent, i.e. 5,5′-dithiobis[2-nitrobenzoic acid]. Altogether, 32 fungal strains from an in-house culture collection were screened. A total of 13 SOX-producing strains were found positive in the plate test screen. The novel SOX producers were Aspergillus tubingensis, Chaetomium globusum, Melanocarpus albomyces, Penicillium aurantiogriseum, Penicillium funiculosum, Coniophora puteana and Trametes hirsuta. Six of the discovered SOX were partially characterised by determination of isoelectric point, pH optimum and substrate specificity. A. tubingensis was identified as the most efficient novel SOX producer.
AB - Sulfhydryl oxidases (SOX) are FAD-dependent enzymes capable of oxidising free thiol groups and forming disulphide bonds. Although the quantity of scientific papers and suggested applications for SOX is constantly increasing, only a limited number of microbial SOX have been reported and are commercially available. Hence, the aim of this study was to develop a fast and reliable qualitative plate test for screening novel secreted fungal SOX. The screening was based on the Ellman's reagent, i.e. 5,5′-dithiobis[2-nitrobenzoic acid]. Altogether, 32 fungal strains from an in-house culture collection were screened. A total of 13 SOX-producing strains were found positive in the plate test screen. The novel SOX producers were Aspergillus tubingensis, Chaetomium globusum, Melanocarpus albomyces, Penicillium aurantiogriseum, Penicillium funiculosum, Coniophora puteana and Trametes hirsuta. Six of the discovered SOX were partially characterised by determination of isoelectric point, pH optimum and substrate specificity. A. tubingensis was identified as the most efficient novel SOX producer.
KW - Ellman's reagent
KW - Enzyme
KW - Fungi
KW - Oxidation
KW - Screening
KW - Sulfhydryl oxidase
KW - Thiol
UR - http://www.scopus.com/inward/record.url?scp=84885954912&partnerID=8YFLogxK
U2 - 10.1007/s00253-013-4753-9
DO - 10.1007/s00253-013-4753-9
M3 - Article
C2 - 23417429
AN - SCOPUS:84885954912
SN - 0175-7598
VL - 97
SP - 9429
EP - 9437
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 21
ER -