Direct laser writing and geometrical analysis of scaffolds with designed pore architecture for three-dimensional cell culturing

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

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Direct laser writing and geometrical analysis of scaffolds with designed pore architecture for three-dimensional cell culturing. / Käpylä, Elli; Aydogan, Dogu Baran; Virjula, Sanni; Vanhatupa, Sari; Miettinen, Susanna; Hyttinen, Jari; Kellomäki, Minna.

julkaisussa: Journal of Micromechanics and Microengineering, Vuosikerta 22, Nro 11, 115016, 01.11.2012.

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

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Käpylä, Elli ; Aydogan, Dogu Baran ; Virjula, Sanni ; Vanhatupa, Sari ; Miettinen, Susanna ; Hyttinen, Jari ; Kellomäki, Minna. / Direct laser writing and geometrical analysis of scaffolds with designed pore architecture for three-dimensional cell culturing. Julkaisussa: Journal of Micromechanics and Microengineering. 2012 ; Vuosikerta 22, Nro 11.

Bibtex - Lataa

@article{a7f6f7d22ae44691b2f3d660176cd9b7,
title = "Direct laser writing and geometrical analysis of scaffolds with designed pore architecture for three-dimensional cell culturing",
abstract = "Traditional scaffold fabrication methods used in tissue engineering enable only limited control over essential parameters such as porosity, pore size and pore interconnectivity. In this study, we designed and fabricated five different types of three-dimensionally interconnected, highly porous scaffolds with precise control over the scaffold characteristics. We used two-photon polymerization (2PP) with a commercial polymer-ceramic material (Ormocomp{\circledR}) for scaffold fabrication. Also for the first time, we analyzed the 2PP fabrication accuracy with respect to scaffold design parameters. Our results showed that the porosity values decreased up to 13{\%} compared to the design specifications due to the fabrication process and the shrinkage of the material. Finally, we showed that our scaffolds supported human adipose stem cell adhesion and proliferation in a six day culture. By precise tuning of scaffold parameters, our design and fabrication method provides a novel approach for studying the effect of scaffold architecture on cell behavior in vitro.",
author = "Elli K{\"a}pyl{\"a} and Aydogan, {Dogu Baran} and Sanni Virjula and Sari Vanhatupa and Susanna Miettinen and Jari Hyttinen and Minna Kellom{\"a}ki",
year = "2012",
month = "11",
day = "1",
doi = "10.1088/0960-1317/22/11/115016",
language = "English",
volume = "22",
journal = "Journal of Micromechanics and Microengineering",
issn = "0960-1317",
publisher = "IOP Publishing Ltd.",
number = "11",

}

RIS - Lataa

TY - JOUR

T1 - Direct laser writing and geometrical analysis of scaffolds with designed pore architecture for three-dimensional cell culturing

AU - Käpylä, Elli

AU - Aydogan, Dogu Baran

AU - Virjula, Sanni

AU - Vanhatupa, Sari

AU - Miettinen, Susanna

AU - Hyttinen, Jari

AU - Kellomäki, Minna

PY - 2012/11/1

Y1 - 2012/11/1

N2 - Traditional scaffold fabrication methods used in tissue engineering enable only limited control over essential parameters such as porosity, pore size and pore interconnectivity. In this study, we designed and fabricated five different types of three-dimensionally interconnected, highly porous scaffolds with precise control over the scaffold characteristics. We used two-photon polymerization (2PP) with a commercial polymer-ceramic material (Ormocomp®) for scaffold fabrication. Also for the first time, we analyzed the 2PP fabrication accuracy with respect to scaffold design parameters. Our results showed that the porosity values decreased up to 13% compared to the design specifications due to the fabrication process and the shrinkage of the material. Finally, we showed that our scaffolds supported human adipose stem cell adhesion and proliferation in a six day culture. By precise tuning of scaffold parameters, our design and fabrication method provides a novel approach for studying the effect of scaffold architecture on cell behavior in vitro.

AB - Traditional scaffold fabrication methods used in tissue engineering enable only limited control over essential parameters such as porosity, pore size and pore interconnectivity. In this study, we designed and fabricated five different types of three-dimensionally interconnected, highly porous scaffolds with precise control over the scaffold characteristics. We used two-photon polymerization (2PP) with a commercial polymer-ceramic material (Ormocomp®) for scaffold fabrication. Also for the first time, we analyzed the 2PP fabrication accuracy with respect to scaffold design parameters. Our results showed that the porosity values decreased up to 13% compared to the design specifications due to the fabrication process and the shrinkage of the material. Finally, we showed that our scaffolds supported human adipose stem cell adhesion and proliferation in a six day culture. By precise tuning of scaffold parameters, our design and fabrication method provides a novel approach for studying the effect of scaffold architecture on cell behavior in vitro.

UR - http://www.scopus.com/inward/record.url?scp=84867961142&partnerID=8YFLogxK

U2 - 10.1088/0960-1317/22/11/115016

DO - 10.1088/0960-1317/22/11/115016

M3 - Article

AN - SCOPUS:84867961142

VL - 22

JO - Journal of Micromechanics and Microengineering

JF - Journal of Micromechanics and Microengineering

SN - 0960-1317

IS - 11

M1 - 115016

ER -

ID: 29135421