Direct laser writing and geometrical analysis of scaffolds with designed pore architecture for three-dimensional cell culturing

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Tutkijat

  • Elli Käpylä
  • Dogu Baran Aydogan
  • Sanni Virjula
  • Sari Vanhatupa
  • Susanna Miettinen
  • Jari Hyttinen
  • Minna Kellomäki

Organisaatiot

  • Tampere University of Technology
  • BioMediTech Finland
  • Tampere University
  • Pirkanmaa Hospital District

Kuvaus

Traditional scaffold fabrication methods used in tissue engineering enable only limited control over essential parameters such as porosity, pore size and pore interconnectivity. In this study, we designed and fabricated five different types of three-dimensionally interconnected, highly porous scaffolds with precise control over the scaffold characteristics. We used two-photon polymerization (2PP) with a commercial polymer-ceramic material (Ormocomp®) for scaffold fabrication. Also for the first time, we analyzed the 2PP fabrication accuracy with respect to scaffold design parameters. Our results showed that the porosity values decreased up to 13% compared to the design specifications due to the fabrication process and the shrinkage of the material. Finally, we showed that our scaffolds supported human adipose stem cell adhesion and proliferation in a six day culture. By precise tuning of scaffold parameters, our design and fabrication method provides a novel approach for studying the effect of scaffold architecture on cell behavior in vitro.

Yksityiskohdat

AlkuperäiskieliEnglanti
Artikkeli115016
JulkaisuJournal of Micromechanics and Microengineering
Vuosikerta22
Numero11
TilaJulkaistu - 1 marraskuuta 2012
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

ID: 29135421