Diagnosing diabetic nephropathy by 1H NMR metabonomics of serum

Ville-Petteri Mäkinen, Pasi Soininen, Carol Forsblom, Maija Parkkonen, Petri Ingman, Kimmo Kaski, Per-Henrik Groop, Mika Ala-Korpela

    Tutkimustuotos: LehtiartikkeliArticleScientificvertaisarvioitu

    59 Sitaatiot (Scopus)

    Abstrakti

    Object: The most severe complication of type 1 diabetes (T1DM) is diabetic nephropathy. It is associated with a high risk of cardiovascular complications and premature death and requires early detection to be efficiently treated. The clinical practice to diagnose diabetic nephropathy is also a non-optimal and tedious set up based on albumin excretion rate in multiple overnight or 24h urine samples. Conversely, in this study, these independent diagnostic data are used to provide a realistic testing case for applying H-1 NMR metabonomics of serum in a diagnostic fashion.

    Materials and Methods: 182 T1DM and 21 non-diabetic (non-T1DM) individuals were studied. The H-1 NMR of serum at 500 MHz was targeted at two molecular windows: lipoprotein lipids and low-molecular-weight metabolites.

    Results: T1DM and non-T1DM individuals were exclusively separated by H-1 NMR. For diabetic nephropathy diagnosis in the T1DM patients, H-1 NMR data (and clinical biochemistry data) gave a sensitivity of 87.1% (83.9%) and a specificity of 87.7% (95.9%). The predictive values of positive and negative tests were 89.0% (95.5%) and 83.6% (79.2%), respectively.

    Conclusions: H-1 NMR metabonomics clearly distinguishes metabolic characteristics of T1DM and appears approximately as good a means to diagnose diabetic nephropathy from serum as an advanced set of biochemical variables.
    AlkuperäiskieliEnglanti
    Sivut281-296
    JulkaisuMagnetic Resonance Materials in Physics, Biology and Medicine
    Vuosikerta19
    Numero6
    DOI - pysyväislinkit
    TilaJulkaistu - 2006
    OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

    Sormenjälki

    Sukella tutkimusaiheisiin 'Diagnosing diabetic nephropathy by 1H NMR metabonomics of serum'. Ne muodostavat yhdessä ainutlaatuisen sormenjäljen.

    Siteeraa tätä