Control of Protein Affinity of Bioactive Nanocellulose and Passivation Using Engineered Block and Random Copolymers

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Control of Protein Affinity of Bioactive Nanocellulose and Passivation Using Engineered Block and Random Copolymers. / Vuoriluoto, Maija; Orelma, Hannes; Zhu, Baolei; Johansson, Leena-Sisko; Rojas, Orlando J.

julkaisussa: ACS Applied Materials and Interfaces, Vuosikerta 8, Nro 8, 2016, s. 5668-5678.

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

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@article{e6a7accdd0a14ae686410bb36fbcf47a,
title = "Control of Protein Affinity of Bioactive Nanocellulose and Passivation Using Engineered Block and Random Copolymers",
abstract = "We passivated TEMPO-oxidized cellulose nanofibrils (TOCNF) toward human immunoglobulin G (hIgG) by modification with block and random copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA). The block copolymers reversibly adsorbed on TOCNF and were highly effective in preventing nonspecific interactions with hIgG, especially if short PDMAEMA blocks were used. In such cases, total protein rejection was achieved. This is in contrast to typical blocking agents, which performed poorly. When an anti-human IgG biointerface was installed onto the passivated TOCNF, remarkably high affinity antibody-antigen interactions were observed (0.90 ± 0.09 mg/m2). This is in contrast to the nonpassivated biointerface, which resulted in a significant false response. In addition, regeneration of the biointerface was possible by low pH aqueous wash. Protein A from Staphylococcus aureus was also utilized to successfully increase the sensitivity for human IgG recognition (1.28 ± 0.11 mg/m2). Overall, the developed system based on TOCNF modified with multifunctional polymers can be easily deployed as bioactive material with minimum fouling and excellent selectivity.",
keywords = "antifouling, biosurfaces, cellulose nanofibrils, human IgG, nonspecific adsorption, PDMAEMA, POEGMA, TEMPO-oxidation",
author = "Maija Vuoriluoto and Hannes Orelma and Baolei Zhu and Leena-Sisko Johansson and Rojas, {Orlando J.}",
year = "2016",
doi = "10.1021/acsami.5b11737",
language = "English",
volume = "8",
pages = "5668--5678",
journal = "ACS Applied Materials and Interfaces",
issn = "1944-8244",
publisher = "AMERICAN CHEMICAL SOCIETY",
number = "8",

}

RIS - Lataa

TY - JOUR

T1 - Control of Protein Affinity of Bioactive Nanocellulose and Passivation Using Engineered Block and Random Copolymers

AU - Vuoriluoto, Maija

AU - Orelma, Hannes

AU - Zhu, Baolei

AU - Johansson, Leena-Sisko

AU - Rojas, Orlando J.

PY - 2016

Y1 - 2016

N2 - We passivated TEMPO-oxidized cellulose nanofibrils (TOCNF) toward human immunoglobulin G (hIgG) by modification with block and random copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA). The block copolymers reversibly adsorbed on TOCNF and were highly effective in preventing nonspecific interactions with hIgG, especially if short PDMAEMA blocks were used. In such cases, total protein rejection was achieved. This is in contrast to typical blocking agents, which performed poorly. When an anti-human IgG biointerface was installed onto the passivated TOCNF, remarkably high affinity antibody-antigen interactions were observed (0.90 ± 0.09 mg/m2). This is in contrast to the nonpassivated biointerface, which resulted in a significant false response. In addition, regeneration of the biointerface was possible by low pH aqueous wash. Protein A from Staphylococcus aureus was also utilized to successfully increase the sensitivity for human IgG recognition (1.28 ± 0.11 mg/m2). Overall, the developed system based on TOCNF modified with multifunctional polymers can be easily deployed as bioactive material with minimum fouling and excellent selectivity.

AB - We passivated TEMPO-oxidized cellulose nanofibrils (TOCNF) toward human immunoglobulin G (hIgG) by modification with block and random copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA). The block copolymers reversibly adsorbed on TOCNF and were highly effective in preventing nonspecific interactions with hIgG, especially if short PDMAEMA blocks were used. In such cases, total protein rejection was achieved. This is in contrast to typical blocking agents, which performed poorly. When an anti-human IgG biointerface was installed onto the passivated TOCNF, remarkably high affinity antibody-antigen interactions were observed (0.90 ± 0.09 mg/m2). This is in contrast to the nonpassivated biointerface, which resulted in a significant false response. In addition, regeneration of the biointerface was possible by low pH aqueous wash. Protein A from Staphylococcus aureus was also utilized to successfully increase the sensitivity for human IgG recognition (1.28 ± 0.11 mg/m2). Overall, the developed system based on TOCNF modified with multifunctional polymers can be easily deployed as bioactive material with minimum fouling and excellent selectivity.

KW - antifouling

KW - biosurfaces

KW - cellulose nanofibrils

KW - human IgG

KW - nonspecific adsorption

KW - PDMAEMA

KW - POEGMA

KW - TEMPO-oxidation

UR - http://www.scopus.com/inward/record.url?scp=84960194037&partnerID=8YFLogxK

U2 - 10.1021/acsami.5b11737

DO - 10.1021/acsami.5b11737

M3 - Article

AN - SCOPUS:84960194037

VL - 8

SP - 5668

EP - 5678

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

SN - 1944-8244

IS - 8

ER -

ID: 1800875