Compressibility and porosity modulate the mechanical properties of giant gas vesicles

Hedar H. Al-Terke, Grégory Beaune, Muhammad Junaid, Jani Seitsonen, Arja Paananen, Jaakko V.I. Timonen, Jussi Joensuu, Françoise Brochard-Wyart*, Robin H.A. Ras

*Tämän työn vastaava kirjoittaja

Tutkimustuotos: LehtiartikkeliArticleScientificvertaisarvioitu

54 Lataukset (Pure)

Abstrakti

Gas vesicles used as contrast agents for noninvasive ultrasound imaging must be formulated to be stable, and their mechanical properties must be assessed. We report here the formation of perfluoro-n-butane microbubbles coated with surface-active proteins that are produced by filamentous fungi (hydrophobin HFBI from Trichoderma reesei). Using pendant drop and pipette aspiration techniques, we show that these giant gas vesicles behave like glassy polymersomes, and we discover novel gas extraction regimes. We develop a model to analyze the micropipette aspiration of these compressible gas vesicles and compare them to incompressible liquid-filled vesicles. We introduce a sealing parameter to characterize the leakage of gas under aspiration through the pores of the protein coating. Utilizing this model, we can determine the elastic dilatation modulus, surface viscosity, and porosity of the membrane. These results demonstrate the engineering potential of protein-coated bubbles for echogenic and therapeutic applications and extend the use of the pipette aspiration technique to compressible and porous systems.

AlkuperäiskieliEnglanti
Artikkelie2211509120
Sivut1-12
Sivumäärä12
JulkaisuProceedings of the National Academy of Sciences of the United States of America
Vuosikerta120
Numero4
DOI - pysyväislinkit
TilaJulkaistu - 24 tammik. 2023
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

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